牛奶脂肪酸及CLA的分析方法的改进

采用完全随机区组试验设计,针对四种不同的牛奶脂肪酸分析方法,按牛奶样品来源分为2个区组,即区组I为8头奶牛个体按每天早、中、晚产奶量加权后的混合样;区组II为60头牛早上挤完奶的混合样,每组16个重复。由于不同CLA异构体间的生物学效果不同,采用常规的气相分离柱(60m)不能完全区分CLA异构体。因此,以Kelly等[1]和Palmquist[2]方法为参照,在利用离心方法和离心加有机溶剂分离乳脂肪并结合酸碱混合甲酯化的基础上,利用进口安捷伦公司HP6890N气相色谱仪结合100m长硅胶毛细管柱(HP2560),采用通过程序升温,气相检测结果表明,该方法能够对乳中常规脂肪酸及其CLA异构体(4种)进行区分,不仅对于乳脂大部分脂肪酸组成没有显著的影响,同时能弥补乳脂肪提取时存在耗时、工作量大、环境污染、成本高和处理效率低的缺陷。该分析方法每天可以预处理300个牛奶样品,能极大地提高分析效率。     

我国粮食供求形势和价格走势判断

一、当前我国粮食供求矛盾有所缓和,但从长远看粮食问题仍面临极大压力(一)目前全国粮食供求形势比较平稳,粮食产需缺口进一步缩小从总体看,2006年我国粮食生产继续稳定发展,粮食消费平稳增长,产需缺口继续缩小,品种结构平衡状况出现新的变化。从生产看,今年国家继续完善和强化     

福建滨海景观规划建设初探

通过对福建省滨海景观资源的分类及滨海景观建设中存在的问题的分析,指出滨海景观的社会与环境价值,并探讨了福建滨海景观建设的原则、对策以及在规划设计中应当注重的生态环境保护等问题。     

养猪场环境细菌总数计数的实验应用模型

分别采用Andersen六级微生物空气样品收集器和LWC-1型离心式空气样品收集器对猪舍空气进行采样，同时采集粪便。然后选用普通营养琼脂和麦康凯选择培养基对所采集的样品进行了细菌培养和细菌总数、大肠杆菌的计数。计数结果为该场舍细菌总数、大肠杆菌数超标比较严重，Ⅱ舍达标，场中心和门口均轻微超标。     

黑羽乌骨鸡碱性磷酸酶的测定

试验以江苏省家禽科学研究所培育的黑羽乌骨鸡为试验素材，测定早期碱性磷酸酶的含量、碱性磷酸酶与早期增重的相关性，试验结果表明：饲养前期（0～6周）碱性磷酸酶的含量极显著地高于饲养后期（7～10周）；饲养全期碱性磷酸酶含量与早期增重呈极显著相关。     

Isolation and identification of Serratia marcescens Ha1 and herbicidal activity of Ha1 ‘pesta’ granular formulation

A total of 479 bacterial strains were isolated from brine(Bohai, Qinhuangdao City, Hebei Province, China). Bioassay results indicated that 4 strains named Ha1, Ha17, Ha38, and Ha384 had herbicidal activity. And strain Ha1 had the highest effective herbicidal activity. As a result, this study aims to identify strain Ha1, characterize its physiological and biological activities, evaluate the herbicidal activity of its metabolites, and develop a ‘pesta' formulation and assess its effectiveness on Digitaria sanguinalis. Ha1 was identified as Serratia marcescens based on 16 S r DNA sequencing. This strain has a flagellum, a diameter of 0.5 to 0.8 μm, and a length of 0.9 to 2.0 μm. The indole test shows positive results, and the catalase enzyme exhibits strong positive reactions. Results further showed that the inhibitory concentration(IC50) of the crude extracts to D. sanguinalis radicula and coleoptile were 3.332 and 2.828 mg m L–1, respectively. Both the suppression of D. sanguinalis and the cell viability of the Ha1 formulation in ‘pesta' were higher when stored at 4°C than at(25±2)°C. These results indicated that S. marcescens Ha1 can potentially be used as a biocontrol agent against D. sanguinalis.     

冬季灌水对土壤中向日葵菌核的活力及其致病力的影响

为明确内蒙古巴彦淖尔市河滩地向日葵菌核病发生轻的原因,在内蒙古农业大学教学农场模拟了巴彦淖尔市河滩向日葵地块的种植条件即对土壤中的向日葵菌核进行了冬季大水漫灌处理,并在次年春天对冬灌水处理后土壤中菌核的活力、草酸分泌量、多聚半乳糖醛酸酶（polygalacturonase,PG）的活性以及致病力进行了测定。结果表明,灌水处理后土壤中菌核萌发率为46%,比未灌水处理的对照降低了27%;草酸分泌量为50.94 μg/mg,PG活性为14.82 U/mg,均显著低于对照。冬灌水处理后的菌核萌发后在离体叶片上接种病斑大小仅为0.69 cm,显著低于未经冬季灌水处理的1.29 cm。2012和2013年的向日葵植株活体接种试验结果表明,用冬灌水处理后的菌核接种后发病株率分别为10.67%和18.37%,显著低于未经灌水但混有菌核的处理。表明冬季灌水处理能够降低土壤中菌核来年对向日葵的致病力,从而降低向日葵菌核病的发病程度。     

太阳能光伏发电技术在温室中的应用

阐述了太阳能光伏电池组件、控制器、逆变器等的设计原则和光伏发电系统的分类。并以北京京鹏环球科技股份有限公司已建的光伏温室为例,论述了光伏发电系统与温室的结合方式。      

Feasibility of open recirculating system in temperate climate – a case study

The increasing number of depleted, overexploited and recovering world marine stocks, together with increasing demand for fish and need for sustainable management of aquatic resources has led to a gradual shift to inland intensive aquaculture with water reuse. Intensive recirculating systems are becoming a rapidly developing sector of aquaculture, with the objectives to increase production and minimize environmental impact. However, transfer of technologies from original sites to locations of different climate is not always successful. The present study evaluates the use of an open recirculating system in a temperate climate. The 3‐year study showed successful production with better fish growth and feed utilization than in a flow‐through facility at the same site, but presented significant issues necessitating changes in technology as well as physical adaptations. A positive effect of the technology with respect to the environment is possible, but systems must be adapted to temperate climatic conditions.