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991.
NO、CO和H2S气体信号分子及其生物学作用已成为当前生命科学领域中激素和信号转导研究的热点。当前,人们正在深入探讨这3种气体信号分子在植物生命活动中的作用。总结了植物内源性气体信号分子的产生方式,参与调节种子萌发、根系发育、叶绿素合成、光合作用、气孔运动、物质代谢、衰老等植物生长发育的过程;以及其在植物应答盐、极端温度、渗透、干旱、重金属等逆境胁迫中的作用。同时,讨论了气体信号分子调控植物生长发育的特点,以及可能的交叉作用途径,并对该领域后续的研究工作进行了展望。 相似文献
992.
994.
皮尔瑞俄类芽胞杆菌BC-39对番茄灰霉病的防治效果及防腐保鲜作用 总被引:2,自引:2,他引:0
为了探索拮抗菌株BC-39对番茄病害的控制作用,采用生长速率法、孢子萌发法测定了BC-39发酵液冻干物对番茄灰霉菌的抑制作用,并研究了菌悬液对盆栽番茄灰霉病的防治效果及其对番茄的防腐保鲜作用。经鉴定拮抗菌株BC-39为皮尔瑞俄类芽胞杆菌Paenibacillus peoriae,其发酵液冻干物抑制番茄灰霉菌菌丝生长和孢子萌发的EC50分别为21.72 μg/mL和23.46 μg/mL;106 CFU/mL BC-39菌悬液对盆栽番茄灰霉病的保护和治疗效果分别为70.1%和65.8%,对采后番茄灰霉病的防腐率为88.4%,经106 CFU/mL菌悬液浸果处理后番茄的失重率为1.34%,低于清水对照。研究表明,皮尔瑞俄类芽胞杆菌BC-39可作为生防制剂应用于番茄灰霉病的防治及番茄的采后保鲜。 相似文献
995.
Temporal variation of characteristic scales in urban landscapes: an insight into the evolving internal structures of China’s two largest cities 总被引:1,自引:1,他引:0
Chi Xu Maosong Liu Chao Hong Ting Chi Shuqing An Xuejiao Yang 《Landscape Ecology》2012,27(7):1063-1074
Urbanization has induced profound landscape changes. While the spatiotemporal patterns of urban landscapes have been extensively studied, the manner by which the internal structures of already urbanized areas change remains little understood. Characteristic scales are an important measure of landscape structure, and they represent the typical spatial extents of landscape elements in hierarchies. In this study, we quantified temporal variations of the characteristic scales in the central urban landscapes of Beijing and Shanghai over an 18?year period. Using transect data from Landsat images, characteristic scales were identified through wavelet analysis and then classified into several discrete domains using the k-means clustering method. These characteristic scale domains appeared to correspond with the typical extents of the blocks and block clusters in the study areas. Results showed that the number of the characteristic scale domains changed within a small range of 3?C5 while the mean values of the characteristic scales within the domains showed substantial temporal variation. Larger characteristic scales were more variable than smaller ones in both cities. Distinguishing relative change rates of building forms, land use and street layout of urban landscapes allowed us to interpret these differences. The street layout of urban landscapes usually reacts slowly to the force of change, acting as the skeleton of the urban landscape. As a result, block sizes can remain relatively stable and corresponding characteristic scales present inheritance features. Land use and building forms are more susceptible to changes. Block clusters with flexible extents could result in significant variation of characteristic scales. 相似文献
996.
Dong-Jun An Wooseog Jeong Hye-Young Jeoung Myoung-Heon Lee Jee-Yong Park Ji-Ae Lim Bong-Kyun Park 《Research in veterinary science》2012,93(1):515-519
A novel peptide nucleic acid (PNA)-based array was developed for use in ante-mortem antigenic typing discrimination in dogs with canine parvovirus (CPV). Cyclic benzothiazole-2-sulfonyl PNA monomers were synthesized that recognized GTA (CPV-2) and TAT (CPV-2a, -2b and -2c) at the nt 913–915 positions, and AAT (CPV-2 and CPV-2a), GAT (CPV-2b), and GAA (CPV-2c) at the nt 1276–1278 positions of the VP2 gene. The detection limits for aa 305 and aa 426 of the VP2 proteins belonging to the four CPV antigenic types were determined optically to be 40–2000 DNA copies, and the optimal cut-off fluorescence signaling value was fixed at 5000. The PNA array described here was developed from 135 field dog fecal specimens and had 89.8% (62/69) sensitivity and 90.4% (66/73) specificity compared with a real-time PCR using the TaqMan assay, a gold standard method. This CPV PNA array could be used together with MGB probe assays as an attractive novel tool for ante-mortem antigenic typing discrimination. 相似文献
997.
998.
X. L. An X. H. Li X. J. Xiong Y. M. Yan Y. Z. Zhang L. Y. Gao A. L.Wang K. Wang F. J. Zeller S. L. K. Hsam 《Plant Breeding》2009,128(1):41-45
A new x-type HMW glutenin subunit, designated as 1Dx1.6t from Aegilops tauschii was identified and characterized by SDS-PAGE and MALDI-TOF-MS. This subunit is located between 1Dx2 and 1Dx1.5t and possesses a molecular mass ( M r ) of 88565.8 Da. Its complete coding sequence was amplified via allele-specific PCR (AS-PCR), and the amplified product was cloned and sequenced. The authenticity of the cloned 1Dx1.6 t gene was confirmed by successful expression of its open reading frame in Escherichia coli. The molecular characterization of 1Dx1.6 t gene showed that its coding region consisted of 2541 bp encoding a polypeptide of 845 amino acid residues. Sequence comparison to previously characterized 1Dx1.5t subunit which is related to good dough quality of bread wheat indicated that the 1Dx1.6t subunit displayed high homology, but possesses 14 residue substitutions and a nonapeptide insertion. A total of 12 single-nucleotide polymorphisms (1 per 212 bp) was identified in the 1Dx1.6 t allele (11 in repetitive domain and 1 in the C-terminal domain), which could facilitate the design of AS-PCR markers. 相似文献
999.
1000.
杨树杂交亲本分子遗传距离与子代生长性状的相关性 总被引:1,自引:0,他引:1
杨树是我国重要的造林树种,在工业人工林和生态防护林建设中发挥着重要作用.目前,我国林业生产中推广应用的杨树良种多数是通过杂交选育出来的,杂种优势明显(符毓秦等,1990;黄东森等,1991;王明庥等,1991;李善文等,2004).根据杂种优势的遗传学原理,获得杂种优势的双亲要存在遗传差异(卢庆善等,2002).杂交育种实践证明,在一定范围内,两亲本间遗传差异越大,其杂种优势也越大,后代分离范围就越广泛,从而获得优良个体的机会也就越多(张爱民,1994).遗传差异评价方法很多,如数量性状遗传距离、亲缘系数、生化标记等. 相似文献