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日本鸡爪槭中苹果根结线虫的鉴定 总被引:1,自引:0,他引:1
宁波口岸连续从来自日本的鸡爪槭根和根围介质中截获一种根结线虫,经形态学和分子生物学方法鉴定为苹果根结线虫。其主要鉴定特征是:雌虫会阴花纹卵圆形,线纹平滑细密,背弓和腹弓均低平,侧尾腺口大,侧尾腺间距大于阴门裂宽;雄虫背食道腺开口到口针基部球的距离较大,平均8μm;2龄幼虫体长和尾长均较短,分别为362~466μm和29.2~39.3μm,c=11.6~15.3。所截获的苹果根结线虫18S rDNA序列与已在GenBank登录的2个苹果根结线虫株系(登录号为EU669948和EU669949)最接近,相似度达99.4%和99.6%。这是我国首次报道截获该种线虫。 相似文献
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应用对rDNA中ITS的PCR-RFLPs技术和方法对来自中国、日本和韩国的松树或木质包装箱中分离出来的伞滑刃属(Bursaphelenchus)6个线虫群体进行了分子鉴定.其中来自浙江镇海的松树和日本的木质包装箱中分离出的各一线虫群体被鉴定为松材线虫(B.xylophilus),而来自浙江富阳的松树和韩国、香港及日本的木质包装箱中的另一个群体等4群体被鉴定为拟松材线虫(B.mucronatus).本研究中选用的4种限制性内切酶(Hinf Ⅰ、Hae Ⅲ、MspⅠ、cfo Ⅰ)的特异性酶切位点均可以有效地鉴别松材线虫和拟松材线虫. 相似文献
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进境意大利苹果苗上葡萄茎枯病菌的截获鉴定 总被引:1,自引:0,他引:1
The isolate ZM40-1 was isolated on Mallus sp. imported from Italy in Ningbo port traditional PDA method. The isolate grew well on three kind of medium, including PDA, MEA and OA, producing mostly little aerial mycelium and abunt pycnidia. The pycnidia were globose or subglobose, 36.4-191.8(87.2) μm in diameter and conidia were variable in shape, mostly ovoid-ellipsoidal, single cell, sometimes curved, 3.3-6.2(5.1)μm ×1.9-3.1(2.7) μm in diameter and hyaline. Chlamydospores were mostly multicellular, solitary or in chains, brown and 18.0-48.0(35.2) μm×7.4-18.7(13.8) μm in size. To compare the isolate sequences (ITS, 28S, ACT and TUB) with the identity of respestive sequence of Phoma glomerata from NCBI GenBank databases by blastn method, the isolate DNA was amplified and sequenced by universal primers. Analysis of these sequences with the related sequences deposited in the NCBI database showed that these sequence had 100% identities, respectively. Pathogenicity tests showed that the isolate didn’t infect stem of Mallus sp.. However, the fruit and leaf were susceptible to P. glomerata. Based on morphological characteristics, molecular and pathogenicity test results, the isolate ZM40-1 was identified as Phoma glomerata (Corda) Wollenweber & Hochapfel. To our know-ledge, this is the first report of interception and identification of P. glomerata in China. 相似文献
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