房地产销售系统的软件设计与实现

房地产业的飞速发展,给房地产销售管理带来了很多新的问题和任务,传统的管理模式已经不能适应现代房地产销售管理的需要,亟待建立一套房地产销售管理信息系统,以满足房地产管理信息化、科学化、现代化的需求。     

农药市场常见违法行为分析与建议

本文对四川省近5年立案查处的农药市场违法案件进行整理,对案例反映出的农药市场常见违法行为进行归类分析,并提出了相应的监管建议,试图给农药市场监管工作提供参考。     

湖南省饭豆地方种质资源遗传多样性的RAPD和ISSR分析

为揭示湖南省饭豆地方品种的遗传多样性,促进种质资源的有效保护和利用。本研究利用RAPD和ISSR标记分析了源于湖南省各地36份古老饭豆地方品种的遗传多样性,并采用UPGMA方法进行了遗传聚类分析。结果表明：12个RAPD引物和4个ISSR引物在供试材料中共扩增产生81条带型清晰的条带,平均每个引物扩增产生5.06个条带;其中59个为多态性条带,占总扩增条带的72.84%,具有多态性引物的PIC值在0.397~0.968之间;通过UPGMA法进行聚类分析表明供试材料的遗传相似系数在0.605~0.877之间,供试材料被分为4大类,且聚类结果表现明显的地域特征。本研究结果可为本省饭豆种质资源保护和育种研究提供理论依据。     

Soybean plant height QTL mapping and meta‐analysis for mining candidate genes

Plant height is closely related to seed yield of soybean. The goal of this study was to identify important loci affecting soybean plant height using meta‐analysis based on a reference physical map. Plant height related to QTLs was mapped across eight years with a RIL population by WinQTLCart v2.5. 182 QTLs related to plant height of soybean from database and our research were collected, and each QTL was projected onto the soybean physical map by software BioMercator v2.1. The confidence interval of meta‐QTL ranged from 0.09 to 5.07 Mb, and the mean phenotypic variance ranged from 4.9% to 73.0%. Furthermore, 4,259 candidate genes were located in these consensus QTLs, and 40 of them were involved in the plant growth and stem elongation and annotated as plant hormone signal transduction (pathway ID ko04075) in KEGG pathway. These results would lay a foundation for fine mapping of QTLs/genes related to plant height and marker‐assisted selection for breeding in soybean.     

农产品储运环境实时监测系统设计

农产品运输车辆和储存仓库的内部温湿度等环境和位置参数是农产品物流调度管理的重要依据，综合使用物联网、无线传感器网络、GPRS无线通信、GSM短信、GPS、GIS等技术与方法，构建一个一体化的农产品储运环境的远程实时监测系统。     

木本园林植物对土壤重金属的富集及修复效应研究进展

我国城市土壤重金属污染形势严峻,已经严重威胁到人类健康,因此受到普遍关注.木本园林植物是城市绿化、建设的主体,是城市生态系统中富有生命力的单元.植物能绿化、美化环境但不会进入食物链,可以对重金属污染土壤进行持续修复,已成为城市土壤污染植物修复的重要研究领域.该研究综述了近年来用于重金属污染土壤修复的木本园林植物,对其修复土壤的强化措施进行了探讨.重点阐述了木本园林植物对各种重金属的富集效应,对其耐受性和净化土壤重金属污染的应用潜力进行了分析,并对未来的发展方向进行了展望.     

以GIS为基础的禽流感预测预警研究进展

GIS是一种对地理环境等方面数据进行储存、分析、加工和呈现的综合工具。利用GIS可以把流感病例的相关数据与气候、环境和社会等数据进行整合和分析，通过统计学或数学模型，找到影响流感发生和传播的风险因素，对流感的发生趋势进行预测和可视化预警。国内外学者利用GIS在禽流感风险因素分析和预测等方面做了大量研究，包括对人感染H7N9流感、高致病性H5亚型禽流感、野禽流感风险因素和预测的研究，不同基因型病毒分布特征的研究，兽医决策辅助应用研究等。这些研究对指导兽医流行病学研究人员将GIS更好地应用于禽流感防控具有帮助意义。     

44个紫花苜蓿品种的酸铝适应性与耐受性评价

为了解紫花苜蓿在贵州地区的适应性及耐酸铝胁迫机理，以44份紫花苜蓿品种为研究对象，研究紫花苜蓿处于酸铝胁迫下的生理变化，并揭示其生理变化与耐酸铝胁迫间的关系。利用基因与环境互作模型对两个地点1年的紫花苜蓿进行产量分析，筛选出阿尔冈金、新疆大叶苜蓿、Trifecta、Vernal和中牧1号苜蓿5个耐酸铝强适应品种。利用敏感型UC-1465和耐受型阿尔冈金进行酸铝胁迫试验。结果表明：相同处理下，耐受型紫花苜蓿的电导率、相对铝含量、死亡率显著低于敏感型；紫花苜蓿对酸铝胁迫的响应主要通过柠檬酸、苹果酸、乙酸、酒石酸、反丁烯二酸和草酸的显著（P<0.05）增加来体现，其中苹果酸的合成和分泌增多可能是其耐酸铝胁迫的重要原因。     

Screening of lentiviral vectors carrying effective siRNA targeting S1P2 gene

AIM:To screen the lentiviral vector carrying siRNA with higher efficiency of suppressing the sphingosine-1-phosphate receptor 2(S1P2) gene expression in the primarily cultured corpus cavernosum smooth muscle cells of spontaneously hypertensive rats (SHR).METHODS:SHR and SD rats (n=5 each) were used for primarily culturing corpus cavernosum smooth muscle cells.The cells were randomly divided into 6 groups:SHR siRNA-1,SHR siRNA-2,SHR siRNA-3,SHR GFP,SHR control (SHR non-transfection group),and SD control (SD rat control group).Each group had 5 samples with 1.0×10⁵ cells of each sample.At 72 h after transfection (MOI=60) with lentiviral vectors carrying S1P2 siRNA into the SHR corpus cavernosum smooth muscle cells,the expression of GFP was observed under fluorescence microscope.The protein expression of S1P2,ROCK1,ROCK2 and eNOS in the corpus cavernosum smooth muscle cells,and the mRNA expression of S1P2,ROCK1 and ROCK2 were determined by by Western blot and RT-PCR.RESULTS:The transfection efficiency of the corpus cavernosum smooth muscle cells in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were>80%.Compared with SHR control group,the mRNA levels and the protein expression of S1P2,ROCK1 and ROCK2 in SHR GFP group showed no remarkable changes,while those in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SD control groups were significantly lower than those in SHR control group (P<0.05).The protein expression of eNOS in SHR siRNA-1,SHR siRNA-2,SHR siRNA-3 and SHR GFP groups were not significantly changed as compared with SHR control group,but that in SD control group was significantly higher than that in SHR control group.CONCLUSION:Three groups of siRNA lentiviral vectors targeting S1P2 inhibit the expression of S1P2 in the corpus cavernosum smooth muscle cells of SHR,and by silencing the S1P2 expression,the expression of ROCK1 and ROCK2 is inhibited.Among them,siRNA-1 has the highest inhibitory efficiency.     

小麦转录因子TaMYB5 like转录自激活能力及其参与生理型花粉败育过程表达模式研究

化学杂交剂SQ-1诱导的小麦花粉败育是一个复杂的生理代谢过程，TaMYB5-like被发现参与此过程。为了解TaMYB5-like基因在SQ-1诱导小麦生理型花粉败育过程中的作用，以SQ-1诱导的小麦生理型雄性不育系PHYMS-1376和对照可育系CK-1376四分体、单核早期、单核晚期、二核期和三核期花药为试验材料，克隆TaMYB5-like基因，并对其进行序列结构、生物信息学、表达模式、亚细胞定位及转录自激活效应分析。结果发现，TaMYB5-like基因序列长5 207 bp，其cDNA长为1 133 bp，其中CDS序列长819 bp，编码272个氨基酸，含有2个SANT结构域，分子量为29.36 kDa，无信号肽，无跨膜结构，亚细胞定位在细胞核内；TaMYB5-like基因启动子涉及光响应、逆境胁迫响应、茉莉酸甲酯响应等顺式作用元件。自激活效应发现，TaMYB5-like蛋白的自激活区段位于N端，合适浓度的AbA和3-AT可以抑制BD-TaMYB5-like和BD-TaMYB5-like-N-SANT融合蛋白的自激活性；随着蛋白质氨基酸序列的截断，自激活性呈降低趋势。与CK-1376相比，PHYMS-1376四分体和单核早期花药中TaMYB5-like表达量差异不显著；花药发育到单核晚期、二核期和三核期时，TaMYB5-like表达量显著增加；CK-1376和PHYMS-1376三核期时花药内TaMYB5-like表达量均最高。