红外辐射技术在农副产品加工中的应用与进展

红外辐射加热干燥技术有众多的优点，近些年来在农副产品加工中的应用越来越多。为此。介绍了红外辐射的基本原理及特点；并通过大量的实例阐述了红外辐射在谷物、蔬菜、竹材加热干燥上的应用与研究进展，以及红外辐射技术在农副产品杀菌方面的应用。     

柴油机燃烧过程及NOx排放的试验研究

测量分析柴油机气缸压力、负荷特性、NOx、HC、CO和烟度等废气排放,并测量分析了供油提前角对动力性、经济性及排放的影响.试验结果表明:供油提前角提前时,柴油机气缸压力增加,NOx增加;负荷增加时,NOx排放浓度增加;但NOx比排放除了在小负荷时较大外,在中等及大负荷时基本保持不变.     

WebGIS客户端实现技术的比较研究及应用

WebGIS(网络地理信息系统)是当前GIS业内最热门的话题之一.WebGIS的开发技术和工具也越来越多.为此,在介绍Applet、栅格图形、VML等几种WebGIS开发的客户端实现技术的同时,对其优缺点进行了比较;分析了目前WebGIS混合实现技术存在的缺陷,并采用其中比较优秀的VML技术,针对环境地理信息进行了试验;最后,对这种VML技术结合与WebGIS的前景进行了讨论,指出了VML技术将在WebGIS的发展过程中起到相当大的作用.     

我国发展精准农业的必要性

精准农业是当今世界农业发展的新潮流,是未来农业发展的雏形。其不仅能提高农产品产量和质量,而且对生态环境能起到良好的保护作用,使农业可持续发展。为此,阐述了精准农业的含义,论述了国内外精准农业的发展现状、研究动态及应用情况,并分析了我国水资源和肥料利用状况,指出了我国发展精准农业的必要性和适合我国国情的精准农业的发展方向。     

农村电网改造规划方法的研究

农村电网的合理规划对节约优化农村有限的资金和能源、减轻农民负担、推动经济发展具有十分重要的意义。新理论和新技术的研究与应用是我国农村电网规划的大势所趋。为此,针对我国农村电网的实际情况,介绍了农网改造的缘由、意义和目的,阐述了电网规划的模型、采用的技术方法及其发展趋势。研究结果对我国现在和将来的农村电网规划与改造具有指导意义和参考价值。     

四川简阳地区砂岩层中水分利用研究

分析了四川简阳地区砂岩和土壤中的水分运动特性和砂岩中储存的水分对土壤水分补给量的多少。对当地的砂岩和土壤进行了水分特征曲线的测定试验、入渗试验和蒸发试验,并采用研究中常用模型对试验结果进行了拟合,通过对拟合结果的分析,得出了当地土壤和砂岩的水分特性,总结出了该地区表面土层在缺水时水分很容易被下部砂岩层补给,砂岩中的水分是作物利用的重要水资源的结论。这对于充分利用当地水资源,合理确定类似地质条件地区灌溉定额,解决无灌溉条件丘陵区作物缺水问题,实现农业节水灌溉有一定的参考价值。     

生活污水厌氧消化出水后处理技术研究进展

本文综述了生活污水厌氧消化系统相关后处理技术的技术特点和研究进展,着重分析了好氧处理、稳定塘处理及土地处理三种后处理技术方法,简要介绍了户用一体化装置的研究及应用状况。     

国外一种最新速度系数资料的插值求解

速度系数法是确定叶轮几何参数的一种常用方法。本文作者以数值分析方法求解了国外一种最新速度系数曲线资料的插值函数．从而为叶轮设计人员提供了一项方便利用国外先进技术．提高叶轮设计质量的有效工具。     

Progress in RNA-binding protein HuR and its roles in development of hepatocellular carcinoma

RNA-binding proteins （RBP） are molecules with a variety of biological functions discovered in recent years. Among them, HuR is an important RBP, widely expressed in various tissues of the body, and is a member of the Hu/embryonic lethal abnormal vision （ELAV） protein family. It mainly affects the expression levels of target genes in the cells by regulating the stability and/or translation efficiency of the mRNA of the genes, thus participating in the regulation of cell life activities. In recent years, more and more studies on HuR have revealed its important role in inflammation and cancer. This review summarizes the functions of HuR and probes into the regulation of HuR functions. In addition, the roles of HuR in the occurrence and development of hepatocellular carcinoma （HCC） are also discussed, which provides important information for exploring the pathogenesis, biomarkers and therapeutic targets of HCC.     

Construction of mouse ESC line stably expressing Pdx1 by Tet-On system

AIM To construct the mouse embryonic stem cell （ESC） line with stable pancreatic and duodenal homeobox 1 （Pdx1） expression by Tet-On system, which may lay a foundation for further research on the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells. METHODS The Pdx1-overexpressing lentiviral vector with green fluorescent protein marker and puromycin resistance was constructed by Tet-On system and was used to infect the mouse ESC. The cells were divided into 3 groups： blank control group （ESC group）, empty lentivirus control group （PDX1^- ESC group） and Pdx1 lentivirus transfection group （PDX1⁺ ESC group）. Flow cytometry was used to detect the transfected cells after screening by doxycycline （DOX）. The function of Tet-On system and the expression of Pdx1 gene were detected. The transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were sorted by flow cytometry, and constructed ESC line with stable expression of Pdx1 and negative control ESC line were verified. RESULTS （1） The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group were 90.72% and 94.01% after screening by DOX, respectively. The positive rates of transfected cells in PDX1^- ESC group and PDX1⁺ ESC group was 97.84% and 98.13% after sorting by flow cytometry, respectively. （2） With DOX, green fluorescence was observed in PDX1^- ESC group and PDX1⁺ ESC group. The mRNA and protein expression of Pdx1 was significantly increased in PDX1⁺ ESC group （P<0.05）. Without DOX, no green fluorescence was observed in the cells of the 3 groups, and no significant difference in the mRNA and protein expression of Pdx1 was observed （P>0.05）. （3） After 3 months of cryopreservation, the cell lines still survived in resuscitation culture and were regulated by DOX. CONCLUSION Using Tet-On system, the mouse ESC line with inducible Pdx1 expression were successfully established and could be used as an effective cell model to research the differentiation of Pdx1⁺ definitive endoderm cells into pancreatic cells.