Recent approaches in nitrogen management for sustainable agricultural production and eco-safety

Among plant nutrients, nitrogen (N) is the most important. Its importance as a growth- and yield-determining nutrient has led to large and rapid increases in N application rates, but often with poor use efficiency. Nitrogen management requires special attention in its use so that the large losses can be minimized and the efficiency maximized. Site-specific nutrient management (SSNM) has been found especially useful to achieve the goals of improved productivity and higher N use efficiency (NUE). Leaf color charts and chlorophyll meters assist in the prediction of crop N needs for rice and wheat, leading to greater N-fertilizer efficiency at various yield levels. Crop simulation models can be used in combination with field information and actual weather data to make recommendations to achieve higher NUE. Remote sensing tools are also used to predict crop N demands precisely. At the same time, traditional techniques like balanced fertilization, integrated N management (INM), use of nitrification inhibitors and slow-release nitrogenous fertilizers (SRNF), split application and nutrient budgeting, among others, are also used to supplement recent N management techniques to attain higher productivity and NUE, and reduce environmental pollution through the leakage of fertilizer N.     

Soil test-based nutrient balancing improved crop productivity and rural livelihoods: case study from rainfed semi-arid tropics in Andhra Pradesh,India

Widespread multinutrient deficiencies in the semi-arid tropics (SAT) are among major factors for large gaps between farmers’ current crop yields and potential yields. In this study, we adopted a stratified soil sampling method to assess soil fertility-related constraints in farmers’ fields in eight districts of Andhra Pradesh in the semi-arid tropics of India. Most of the fields across all eight districts were critical in sulfur (61%–98% deficient fields); and up to six districts each in boron (83%–98% deficient fields), zinc (50–85% deficient fields), and soil organic carbon (55–97% deficient fields). Low soil organic carbon specifically indicates nitrogen deficiency. Phosphorus deficiency was critical in three districts (60–84%) while potassium in general was adequate. Soil test-based nutrient balancing through the application of sulfur, boron, and zinc in addition to farmers’ practice of adding only nitrogen, phosphorus, and potassium increased crop productivity by 8%–102%. Benefit–cost ratio (1.60–28.5) proved favourable to scale-up balanced nutrition. Better post-harvest soil health and residual benefits of sulfur, boron, and zinc up to four succeeding seasons indicated sustainability of the practice. Results showed that balanced nutrition is a way forward for sustainably improving farm productivity and livelihoods.     

Persistence of metalaxyl and mancozeb on potato leaves and their residues in tubers

The persistence of fungicides on two commercial cultivars of potato was determined under field conditions at Punjab Agricultural University, Ludhiana, Punjab. Initial deposits of mancozeb on potato leaves were found to be 26.9 and 38.7 mg kg(-1), following application of ready mixture of fungicide metalaxyl 8% + mancozeb 64% (Ridomil MZ) at the rate of 1260 and 2520 g a.i. ha(-1), whereas metalaxyl residues were found to be 35.1 and 49.5 mg kg(-1), respectively. The residue level of mancozeb in potato leaves 15 days after application at single and double doze were 19.0 and 27.0 mg kg(-1) showing a loss of 29.6 and 30.3%, whereas the values for metalaxyl at single and double doze were 0.40 and 0.80 mg kg(-1) showing a loss of 98.9 and 98.4%, respectively. Residues of mancozeb and metalaxyl were not detected at 0.04 and 0.02 mg kg(-1) level in potato tubers at harvest (PHI = 53 days) at both the dosages, respectively. The persistence and dissipation of mancozeb with the application of Ridomil MZ followed similar trend as in Indofil M-45. The rate of fungicide dissipation increased with time after application in both the potato cultivars 'Kufri Chandramukhi' and 'Chipsona'. No significant difference was observed on initial deposit, persistence and dissipation of the two molecules between the two potato cultivars.     

In Vitro Maturation of Dromedary (Camelus dromedarius) Oocytes: Effect of Different Protein Supplementations and Epidermal Growth Factor*

The present experiment was aimed to compare the effect of different protein supplementation sources, foetal calf serum (FCS), oestrous dromedary serum (EDS) and BSA, in experiment 1, and the effect of different concentrations of epidermal growth factor (EGF), in experiment 2, on in vitro nuclear maturation of the dromedary oocytes. Cumulus oocyte complexes (COCs) were harvested from the ovaries collected from a local slaughterhouse by aspirating the visible follicles in PBS supplemented with 5% FCS. Pooled COCs were randomly distributed to 4‐well culture plates containing 500 μl of the maturation medium and cultured at 38.5°C in an atmosphere of 5% CO₂ in air for 32–36 h. The basic maturation medium consisted of TCM‐199 supplemented with 0.1 mg/ml L‐glutamine, 0.8 mg/ml sodium bicarbonate, 0.25 mg/ml pyruvate, 50 μg/ml gentamicin, 10 μg/ml bFSH, 10 μg/ml bLH and 1 μg/ml estradiol. In experiment 1, this medium was supplemented with 10% FCS, 10% EDS or 0.4% BSA, whereas in experiment 2, it was supplemented with 0.4% BSA and 0, 10, 20 or 50 ng/ml of EGF. The oocytes were fixed, stained with 1% aceto‐orcein stain and their nuclear status was evaluated. Oocytes were classified as germinal vesicle, diakinesis, metaphase‐I, anaphase‐I (A‐I), metaphase‐II (M‐II) and those with degenerated, fragmented, scattered, activated or without visible chromatin as others. There was no difference (p > 0.05) observed in the proportion of oocytes reaching M‐II stage between the media supplemented with FCS (71.5 ± 4.8), EDS (72.8 ± 2.9) and BSA (72.7 ± 6.2). In experiment 2, a higher proportion (p < 0.05) of oocytes reached M‐II stage when the medium was supplemented with 20 ng/ml of EGF (81.4 ± 3.2) when compared with the media supplemented with 10 ng/ml (66.9 ± 4.1) and control (67.2 ± 7.1) groups. It may be concluded that the maturation media for dromedary camel oocytes can be supplemented with any of the three protein sources, i.e. FCS, EDS and BSA without any significant differences on the maturation rates. Also, a supplementation of 20 ng/ml of EGF in the maturation medium seems to be optimal and improves the nuclear maturation of dromedary camel oocytes.     

Managing natural resources of watersheds in the semi-arid tropics for improved soil and water quality: A review

Soil, water and production systems constitute the most important natural resources of a watershed in the rainfed agro-ecosystem; and for sustainability of the production systems they need to be in harmony with the environment. To learn from the past research, a review is made of literature on the impact of natural resource management practices on soil and water quality in the semi-arid tropical regions of India. The results from long-term on station field experiments show that an integrated use of soil and water conservation practices with balanced plant nutrition can not only sustain increased productivity but also maintain soil quality at the watershed or catchment level. Natural resource management practices that conserve soil and water also help to maintain surface and groundwater quality. The changes in soil and water quality, as impacted by natural resource management practices, need to be monitored and assessed on a continuing basis as the outcome of such research offers valuable opportunity for the implementation of corrective management practices, as and when needed.     

Synergistic effects of the inoculation with nitrogen‐fixing and phosphate‐solubilizing rhizobacteria on the performance of field‐grown chickpea

The synergistic effects of nitrogen‐fixing and phosphate‐solubilizing rhizobacteria on plant growth, yield, grain protein, and nutrient uptake of chickpea plants were determined in a sandy clay‐loam soil. Legume grain yield and concentration and uptake of nitrogen (N) and phosphorus (P) were significantly increased as a result of co‐inoculation with Mesorhizobium and P‐solubilizing Pseudomonas and Bacillus spp. The inoculation with M. ciceri RC4 + A. chroococuum A10 + Bacillus PSB9 tripled the seed yield and resulted in highest grain protein (295 mg g^–1) at 145 d after sowing (DAS). An 8% increase in P concentration above the uninoculated control was observed in case of a single inoculation with Pseudomonas PSB 5, while the P uptake was highest (2.14‐fold above the uninoculated control) with a combined inoculation with [M. ciceri RC4 + A. chroococcum A10 + Bacillus PSB 9] at 145 DAS. The highest N concentration and N uptake at 145 DAS (81% and 16% above the uninoculated control, respectively) were observed with the triple inoculation of [M. ciceri RC4 + A. chroococcum A10 + Pseudomonas PSB 5). These findings show that multiple inoculations with rhizospheric microorganisms can promote plant growth and grain yield and increase concentrations and uptake of N and P by field‐grown chickpea.     

Widespread Deficiencies of Sulfur,Boron, and Zinc in Indian Semi-Arid Tropical Soils: On-Farm Crop Responses

On-farm studies were conducted during 2002–2004 to determine fertility status, including sulfur (S) and micronutrients, and crop response to fertilization on farmers' fields in the semi-arid zone of India. Nine hundred-twenty four soil samples taken from farmers' fields, spread in the three districts of Andhra Pradesh (India), were analyzed for soil chemical fertility parameters. Results showed that samples were low in organic carbon (C), total nitrogen (N), and low to moderate in extractable phosphorus (P), but adequate in available potassium (K). Analyses of soil samples for extractable S and micronutrients was most revealing and showed that 73–95% of the farmers' fields were deficient in S, 70–100% in boron (B), and 62–94% in zinc (Zn). On-farm trials conducted during three seasons (2002–2004) showed significant yield responses of maize, castor, groundnut, and mung bean to the applications of S, B, and Zn. The yield responses were larger when S, Zn, and B were applied along with N and P. Applications of S, B and Zn also significantly increased the uptake of N, P, K, S, B, and Zn in the crop biomass. Results show widespread deficiencies of S, B, and Zn under dryland agricultural conditions; results also show that the nutrient deficiencies can be diagnosed by soil testing. It was concluded that the drylands in the semi-arid regions of India were not only thirsty (water shortage), but also hungry (nutrient deficiencies).     

Is There a Two‐Humped Stage in the Embryonic Development of the Dromedary?

It has been postulated that the one‐humped (Arabian) dromedary and the two‐humped (Bactrian) camel originated from a single ancestor. Consequently, the dromedary was considered a breed of the two‐humped camel, based on an anatomical study by Lombardini L, 1879: Ann. Del. Universita Toscane, 259 , 147, who described a reduced second hump like structure in foetal and adult dromedaries. To resolve this lingering issue, we analysed dromedary foetuses and calves. In contrast to the situation in two‐humped camels, we never observed any rudimentary second hump in the dromedary foetuses or calves.     

Comparative Evaluation of Inductively Coupled Plasma-Optical Emission Spectrometry and Colorimetry for Determining Phosphorus in Grain Samples

The inductively coupled plasma (ICP)-based method provides the opportunity to determine phosphorus (P) along with other major, secondary, micro and trace elements in plant materials. This study was conducted to compare and evaluate the relative efficacy of the inductively coupled plasma-optical emission spectrometry (ICP-OES) method with that of the colorimetric method using Skalar autoanalyzer, for determining P in 428 grain samples of eight diverse crops. The results on grain P analysis by the two methods, for individual as well as for all crop samples combined, showed that they were highly positively correlated (r varied from 0.84 to 0.98, p < 0.0001 for the eight crops, and R² for all crop grain samples was 0.9201, p < 0.0001). Moreover, the precision by the ICP method was similar to that determined by the Skalar method. Our results demonstrate that the ICP-OES method can be conveniently used for determining P along with other plant nutrient elements in grain samples of diverse crops.