High deleterious genomic mutation rate in stationary phase of Escherichia coli

In natural habitats, bacteria spend most of their time in some form of growth arrest. Little is known about deleterious mutations in such stages, and consequently there is limited understanding of what evolutionary events occur. In a deleterious mutation accumulation experiment in prolonged stationary phase of Escherichia coli, about 0.03 slightly deleterious mutations were observed per genome per day. This is over an order of magnitude higher than extrapolations from fast-growing cells, but in line with inferences from observations in adaptive stationary phase mutation experiments. These findings may affect understanding of bacterial evolution and the emergence of bacterial pathogenicity.     

Role of Hec1 in spindle checkpoint signaling and kinetochore recruitment of Mad1/Mad2

The spindle checkpoint delays sister chromatid separation until all chromosomes have undergone bipolar spindle attachment. Checkpoint failure may result in chromosome mis-segregation and may contribute to tumorigenesis. We showed that the human protein Hec1 was required for the recruitment of Mps1 kinase and Mad1/Mad2 complexes to kinetochores. Depletion of Hec1 impaired chromosome congression and caused persistent activation of the spindle checkpoint, indicating that high steady-state levels of Mad1/Mad2 complexes at kinetochores were not essential for checkpoint signaling. Simultaneous depletion of Hec1 and Mad2 caused catastrophic mitotic exit, making Hec1 an attractive target for the selective elimination of spindle checkpoint-deficient cells.     

Mineral nutrition and water use patterns of a maize/cowpea intercrop on a highly acidic soil of the tropic semiarid

Due to global warming, water is expected to become scarce especially in semiarid regions. Therefore, there is a need to increase the efficiency in water use by crops under rainfed agriculture. The effect of nutrient availability on the growth, production, root development, water relations and water use efficiency (WUE) by the intercrop maize/cowpea was investigated in 2 contrasting years (dry and wet) in the semiarid region of Brazil. The crops were grown on a strongly acidic, sandy soil with three treatments: (i) application of NPK fertilizers plus lime (NPK + lime), (ii) application of NPK fertilizers (NPK) and (ii) control (Contr.) in low and high input regimes. The soil water balance was calculated with the crop model EPICSEAR. Application of fertilizers and lime increased biomass production and grain yield of the intercrop up to 400% and 550%, respectively, and maize suffered more from the effects of low nutrient availability and soil acidity than cowpea. The root development of both crops was strongly improved by the application of NPK and lime and cowpea developed a deeper root system which enabled this crop to keep a higher transpiration rate in the dry year. As a consequence of the shallow root system, maize was prone to water stresses caused by the dry spells and its harvest index was reduced when dry spells occurred during flowering and grain filling.     

Chemical deterrents in podurid Collembola

Four aromatic compounds (2,4-dimethoxyaniline, 1,3-dimethoxybenzene, 2-aminophenol, phenol) found in Neanura muscorum (Collembola, Neanuridae) have been investigated for their role as deterrents against the predatory mite Pergamasus norvegicus (Acarina, Parasitidae). 2-Aminophenol has deterring properties against P. norvegicus, the other substances show no effect as single substances nor is there a synergistic effect.The reaction of three predators, Microvelia reticulata (Heteroptera, Veliidae), Gambusia affinis (Teleostei, Cyprinodontidae) and Rana temporaria (Amphibia, Ranidae) on Podura aquatica (Collembola, Poduridae) is reported. The strongest deterring effects of P. aquatica could be observed with G. affinis. M. reticulata also shows behavioural changes that can be attributed to deterring effects of P. aquatica. The collembolan has no deterring effects on R. temporaria.A synopsis of literature dealing with deterring effects of collembola on arthropod predators is given.     

Genetic variation in testcrosses of European maize inbreds for resistance to the European corn borer and relations to line per se performance

The European com borer (ECB) Ostrinia nubilalis H. is a major pest in World maize Zea mays L. production. Objectives of this study were to (1) investigate the genotypic variance and covariance in testcrosses of European flint and dent inbreds for ECB resistance and agronomic traits, and (2) estimate the correlation between line per se and testcross performance for ECB resistance traits. A total of 16 flint and 24 dent inbreds and their testcrosses with two testers from the opposite germplasm pool were evaluated in four and three German environments, respectively. Using artificial infestation with ECB larvae, resistance was assessed by damage rating of stalks, tunnel length in dissected stalks, and relative yield of infested plots compared with protected plots. Yield losses due to ECB damage in testcross hybrids amounted to 40%. Significant genotypic variances between flint and dent lines and high heritabilities were found for damage rating of stalks for both line per se and testcross performance. Heritabilities were low or intermediate for tunnel length and relative grain yield. Correlations between line per se and testcross performance were tight for the damage rating of stalks and moderate for tunnel length and relative yield in both flint and dent germplasm. For damage rating of stalks, per se performance of lines tested in a few environments can be used to predict their testcross performance. In contrast, assessment of testcross performance for tunnel length and relative yield requires evaluating testcrosses with several testers in multi-environment trials.     

Hormone Profiles of Captive Striped Bass Morone saxatilis During Spermiation, and Long-Term Enhancement of Milt Production

Abstract— Plasma profiles of reproductive and thyroid hormones were studied in captive striped bass Morone saxatilis during an 11-wk period encompassing the spawning season, and the effect of a sustained-release gonadotropin-releasing hormone agonist (GnRHa)-delivery system (GnRHa-implant) on milt production was evaluated. The highest percentage of spermiating fish was observed between mid-April and mid-May, and mean total expressible milt ranged from 3.5 to 6.0 mL/kg. Plasma gonadotropin II (GtH II) increased significantly, though inconsistently, during the spermiation period, whereas testosterone and 11-ketotestosterone levels declined continually. Plasma 17,20β-dihydroxy-4-pregnen-3-one and 17,20β,21-dihydroxy-4-pregnen-3-one remained low and unchanged during the peak of the spermiation period, while thyroid hormones were high and fluctuated without exhibiting a trend consistent with spermiation. The observed endocrine profiles suggest that captivity can diminish plasma GtH II and triiodothyronine levels in striped bass. Transfer of spermiating males from large holding tanks to small spawning tanks reduced total expressible milt after 14 d, but treatment with a GnRHa-implant restored milt volume, presumably due to the prolonged elevation of plasma GnRHa and GtH II induced by the GnRHa-implant. Also, treatment with the GnRHa-implant induced a two- to four-fold elevation of expressible milt for at least 20 d compared to control fish, while resulting in only a 5 to 15% decrease in sperm density. It appears that captivity and hatchery operations can diminish milt production in striped bass, and that GnRHa-delivery systems, via sustained elevation of plasma GtH II, can induce long-term enhancement in milt volume without affecting sperm density greatly.     

Behaviour,escapement and mortality of female European silver eels within a regulated lowland river draining into the Baltic Sea

Migration patterns of 146 female silver eels on their way through fresh and brackish water stretches of the Warnow River into the Baltic Sea were studied using acoustic telemetry. The majority of eels (77%) regained their expected downstream migration behaviour after a median rehabilitation time of 2 days. Positive rheotactic behaviour was observed for 23% of tagged eels after release, probably influenced by experimental procedures. The observed silver eel escapement rate was 63%. Two migratory groups were classified by significantly different migratory speed, time of residence in brackish waters and biometric silvering characteristics. Although the classification of silvering stages is based on these measures, the proportions of these stages did not reflect the observed differences in migratory behaviour. In the context of a permanent silver eel descent in the freshwater part of the Warnow River, a permanent escapement was likewise occurring into the Baltic Sea.     

Are dietary genistein and equol potent enhancers of eicosapentaenoic acid and docosahexaenoic acid levels in rainbow trout (Oncorhynchus mykiss)?

Phytoestrogens are putatively able to enhance the biosynthesis of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), but have also been shown to affect fish growth dose dependently. The aim of the present study was to identify a concentration for the phytoestrogen genistein and the phytoestrogen metabolite equol that further increases the endogenous biosynthesis of EPA and DHA without impairing fish growth. Juvenile rainbow trout (87.2 ± 0.3 g) were fed seven diets on a fixed ratio for 8 weeks. A vegetable oil‐based diet served as a control diet and was supplemented with equol (EQ) and genistein (G), respectively, at 0.1%, 0.2% and 0.3% of feed dry matter (1, 2 and 3). Growth and nutrient composition of whole body homogenates were not affected by dietary treatments. EPA and DHA levels in liver, fillet and whole body samples were not significantly increased by EQ and G diets. Fish fed EQ diets showed dose dependently increased liver weights and C18:0 liver levels, indicating estrogen‐like effects at increased dietary dosages. In conclusion, the utilization of equol and genistein in plant oil‐based diets in order to enhance the biosynthesis of EPA and DHA seems not reasonable in rainbow trout.     

Development options of natural habitats in a post‐mining landscape

Rehabilitation of the post‐mining landscape of Lower Lusatia (Brandenburg, Germany) represents a complex problem: 85 per cent of this area is currently being recultivated for forests or agriculture and 15 per cent is reserved for nature development. After mining, soil conditions are dry, acidic and nutrient poor. The ‘LENAB’ project aimed at conservation strategies and management options for natural habitats. Two competing basic motives of nature conservation on a small or medium scale were defined: (1) close‐to‐naturalness (allowing natural dynamics to take place without human interference) and (2) biodiversity (selective species and habitat protection). An overall strategy for sustainability of a cultural landscape can only be applied to the entire post‐mining landscape. The application of the two basic motives leads to different management options; this is illustrated by two important vegetation types. Copyright © 2000 John Wiley & Sons, Ltd.     

Correction to: Maintenance requirement and utilisation efficiency of energy and protein for ongrowing pikeperch (Sander lucioperca (L.))

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