全文获取类型
收费全文 | 550篇 |
免费 | 33篇 |
专业分类
林业 | 29篇 |
农学 | 21篇 |
基础科学 | 1篇 |
78篇 | |
综合类 | 40篇 |
农作物 | 135篇 |
水产渔业 | 70篇 |
畜牧兽医 | 174篇 |
园艺 | 8篇 |
植物保护 | 27篇 |
出版年
2023年 | 1篇 |
2022年 | 6篇 |
2021年 | 24篇 |
2020年 | 12篇 |
2019年 | 14篇 |
2018年 | 17篇 |
2017年 | 10篇 |
2016年 | 26篇 |
2015年 | 20篇 |
2014年 | 29篇 |
2013年 | 33篇 |
2012年 | 47篇 |
2011年 | 58篇 |
2010年 | 29篇 |
2009年 | 26篇 |
2008年 | 32篇 |
2007年 | 37篇 |
2006年 | 28篇 |
2005年 | 26篇 |
2004年 | 21篇 |
2003年 | 16篇 |
2002年 | 15篇 |
2001年 | 14篇 |
2000年 | 8篇 |
1999年 | 7篇 |
1998年 | 3篇 |
1997年 | 2篇 |
1996年 | 1篇 |
1995年 | 1篇 |
1994年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1990年 | 1篇 |
1989年 | 2篇 |
1986年 | 1篇 |
1985年 | 2篇 |
1981年 | 1篇 |
1979年 | 1篇 |
1975年 | 1篇 |
1964年 | 1篇 |
1963年 | 1篇 |
排序方式: 共有583条查询结果,搜索用时 17 毫秒
71.
Exploring genetic diversity of rice cultivars for the presence of brown planthopper (BPH) resistance genes and development of SNP marker for Bph18 下载免费PDF全文
Gandhimani Ramkumar G. D. Prahalada Sherry Lou Hechanova Sung‐Ryul Kim Kshirod K. Jena 《Plant Breeding》2016,135(3):301-308
Brown planthopper (BPH) is the most devastating insect pest in rice‐growing areas. Information on availability of BPH resistance alleles and their sources enhances BPH‐resistant breeding programmes. In this study, 260 highly diversified rice cultivars or breeding lines were screened for the presence of five major BPH resistance genes (Bph10, Bph13, Bph18, Bph20 and Bph21) using gene‐specific markers. The analysis revealed that 137 of the 260 cultivars possess at least one BPH resistance gene. Bph10 was predominant while Bph20 was the least distributed. Moreover, two and three different resistance gene combinations were found in the cultivars. Molecular markers play an important role in molecular breeding programmes. A tightly linked PCR‐based co‐dominant Bph18 marker was developed, which is cost effective and time effective and simpler than available Bph18 CAPS marker (7312.T4A). We strongly believe that the identified BPH‐resistant cultivars can be used as alternative resistance gene sources and also as resource for novel BPH resistance genes. The developed Bph18 marker will be highly useful in molecular breeding applications of BPH‐resistant breeding programmes. 相似文献
72.
Yin-Di Su Tzu-Rong Su Zhi-Hong Wen Tsong-Long Hwang Lee-Shing Fang Jih-Jung Chen Yang-Chang Wu Jyh-Horng Sheu Ping-Jyun Sung 《Marine drugs》2015,13(2):1037-1050
Two new briarane-type diterpenoids, briarenolides K (1) and L (2), were isolated from an octocoral identified as Briareum sp. The structures of new briaranes 1 and 2 were elucidated by spectroscopic methods. In the in vitro anti-inflammatory effects test, briaranes 1 and 2 were found to significantly inhibit the accumulation of the pro-inflammatory iNOS protein of the lipopolysaccharide (LPS)-stimulated RAW264.7 macrophage cells. 相似文献
73.
So Young Kim Myoung-Sook Shin Geum Jin Kim Hyukbean Kwon Myong Jin Lee Ah-Reum Han Joo-Won Nam Chan-Hun Jung Ki Sung Kang Hyukjae Choi 《Marine drugs》2021,19(8)
The epithelial–mesenchymal transition (EMT) of cancer cells is a crucial process in cancer cell metastasis. An Aquimarina sp. MC085 extract was found to inhibit A549 human lung cancer cell invasion, and caprolactin C (1), a new natural product, α-amino-ε-caprolactam linked to 3-methyl butanoic acid, was purified through bioactivity-guided isolation of the extract. Furthermore, its enantiomeric compound, ent-caprolactin C (2), was synthesized. Both 1 and 2 inhibited the invasion and γ-irradiation-induced migration of A549 cells. In transforming growth factor-β (TGF-β)-treated A549 cells, 2 inhibited the phosphorylation of Smad2/3 and suppressed the EMT cell marker proteins (N-cadherin, β-catenin, and vimentin), as well as the related messenger ribonucleic acid expression (N-cadherin, matrix metalloproteinase-9, Snail, and vimentin), while compound 1 did not suppress Smad2/3 phosphorylation and the expression of EMT cell markers. Therefore, compound 2 could be a potential candidate for antimetastatic agent development, because it suppresses TGF-β-induced EMT. 相似文献
74.
Sung Dae LEE Hoi Yun KIM Hyun Jung JUNG Sang Yun JI Rekha CHOWDAPPA Ji Hee HA Young Min SONG Jun Cheol PARK Hong Kil MOON In Cheul KIM 《Animal Science Journal》2009,80(1):79-84
The objective of the present study was to investigate the effect of fermented apple diet (FAD) supplementation on the growth performance and meat quality in finishing Berkshires. The FAD was made from dropped apple mixed with rice bran and barley bran. Until 81 ± 1 kg live weight at 133 ± 1 days, the animals were fed a growing diet, after which experimental samples were fixed at 0, 2, 4 and 6% FAD as C, T1, T2 and T3 in the finishing diets. Growth performance, ADG, ADFI and feed efficiency were improved in T1 than other groups. In carcass parameters, carcass weight was higher ( P < 0.05) in T1 than in other groups. In meat quality, moisture and crude protein contents decreased ( P < 0.05) by addition of FAD. pH24 and WHC were higher ( P < 0.05) in T1 than other groups. In sensory evaluation, marbling of fresh meat and tenderness, juiciness, flavor and overall acceptability of cooked meat were improved by the addition of FAD. According to the results of our experiment, FAD can be used for improvement of meat quality parameters. 相似文献
75.
Gillian A. Perkins Laura B. Goodman Koji Tsujimura Gerlinde R. Van de Walle Sung G. Kim Edward J. Dubovi Nikolaus Osterrieder 《Veterinary microbiology》2009,139(3-4):375-378
A single nucleotide polymorphism in the equine herpesvirus 1 (EHV-1) DNA polymerase gene (ORF30 A2254 to G) has been associated with clinical signs of equine herpes myeloencephalopathy (EHM). The purpose of our study was to determine the odds ratio for this genetic marker and EHM using a panel of field isolates from North America collected over the past twenty-three years. EHV-1 isolates cultured at the Cornell University Animal Health Diagnostic Laboratory from 1984 to 2007 were retrieved along with their clinical histories. DNA was extracted from these EHV-1 cultures and allelic discrimination was performed using real-time PCR. The results were confirmed by sequencing of the target region in ORF30. PCR and sequencing were in 100% agreement and showed that 19 out of the 176 isolates had the ORF30 G2254 allele (11%), of which16 were EHM cases and 3 respiratory or abortion cases. The odds of having neurologic disease with the ORF30 G2254 genotype were computed as 162 times greater than those with the opposite allele ORF30 A2254 (95% confidence interval: 35–742). Despite this strong statistical significance, 24% (5/21) of horses with neurologic disease in our study population harbored the “non-neurologic” form of the allele (ORF30 A2254), suggesting that other factors may also contribute to the onset of EHM. 相似文献
76.
PH Hong YD Su JH Su YH Chen TL Hwang CF Weng CH Lee ZH Wen JH Sheu NC Lin YH Kuo PJ Sung 《Marine drugs》2012,10(5):1156-1168
Two new briarane diterpenoids, briarenolides, F (1) and G (2), were isolated from an octocoral identified as Briareum sp. The structures of briaranes 1 and 2 were established by spectroscopic methods and by comparison of the spectroscopic data with those of known briarane analogues. Briarenolide F was proven to be the first 6-hydroperoxybriarane derivative and this compound displayed a significant inhibitory effect on the generation of superoxide anion by human neutrophils. 相似文献
77.
A novel 15C compound, pseudoalteromone B (1), possessing a novel carbon skeleton, was obtained from a marine bacterium Pseudoalteromonas sp. CGH2XX. This bacterium was originally isolated from a cultured-type octocoral Lobophytum crassum, that was growing in cultivating tanks equipped with a flow-through sea water system. The structure of 1 was established by spectroscopic methods. Pseudoalteromone B (1) displayed a modestly inhibitory effect on the release of elastase by human neutrophils. 相似文献
78.
Li-Hsueh Wang Jyh-Horng Sheu Shih-Yao Kao Jui-Hsin Su Yung-Husan Chen Yu-Hsin Chen Yin-Di Su Yu-Chia Chang Lee-Shing Fang Wei-Hsien Wang Yang-Chang Wu Ping-Jyun Sung 《Marine drugs》2012,10(11):2415-2434
The structures, names, bioactivities and references of 105 natural products obtained from gorgonian corals belonging to the family Plexauridae with an Indo-Pacific distribution are described in this review. All compounds mentioned in this review were obtained from gorgonian corals belonging to the genera Astrogorgia, Bebryce, Echinomuricea, Euplexaura and Menella. 相似文献
79.
Major fish bacterial diseases in Korea are edwardsiellosis, streptococcosis, and vibriosis. Among vibrionaceae, Listonella anguillarum, Vibrio harveyi, V. ichthyoenteri, and Photobacterium damselae were identified as causative organisms of vibriosis in flounder. In this study, we developed a multiplex PCR method using the RNA polymerase β subunit (rpoB) gene, known as a housekeeping gene for identification of Vibrio spp. causing vibriosis in flounder. Three pairs of PCR primers were designed based on the rpoB sequence of three species, V. harveyi, V. ichthyoenteri, and P. damselae. The PCR assay, using a mixture of six primers, yielded amplicons of 601, 434, and 533 bp in V. harveyi, V. ichthyoenteri, and P. damselae. None of the untargeted species yielded an amplicon. The detection limits for pure culture in kidney were 2.5 × 104 cfu/g kidney for V. harveyi, 2.5 × 105 cfu/g kidney for V. ichthyoenteri, and 2.5 × 106 cfu/g kidney for P. damselae. From the colonies on TCBS agar plates of different samples, 632 Vibrio spp. isolated from aquacultured flounder between 2004 and 2010 were identified by the multiplex PCR method. As a result, 265 strains (41.9 %) were V. ichthyoenteri; 115 strains (18.2 %) were V. harveyi and 72 strains (11.4 %) were P. damselae. 相似文献
80.
Binod Prasad Luitel Na-Young Ro Ho-Cheol Ko Jung-Sook Sung Ju-Hee Rhee On-Sook Hur 《Journal of Crop Science and Biotechnology》2018,21(5):499-506
A total of 47 core collections of pepper (Capsicum chinense Jacq.) conserved in National Agrobiodiversity Center (NAC) were studied under field condition at Jeonju over two consecutive years (2015 and 2016). All accessions were characterized for their 14 qualitative and 16 quantitative characters. Results revealed that both qualitative and quantitative characters exhibited wide variation among the studied germplasm. Distribution of fruit characters (fruit length, width, and fruit wall thickness) among the accessions was positively skewed. Of the 47 accessions evaluated, 38.3% accessions had conical shaped fruits and mature fruit color was predominantly red (51.1%), orange (21.3%) and yellow (14.9%). Principal component analyses revealed that (i) 56.64% of the qualitative (fruit shape, color and fruit surface) variation and (ii) 89.42% of the quantitative (plant width, height and fruit maturity days) variation were explained by the first two components. Clustering revealed two groups and dendrogram revealed morphological variation among accessions. The phenotypic diversity exists in this core collections provide valuable information to improve agronomic traits in pepper breeding program. 相似文献