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91.
Inhibitory effect of (-)-epigallocatechin 3-gallate, a polyphenol of green tea, on neutrophil chemotaxis in vitro and in vivo 总被引:1,自引:0,他引:1
Takano K Nakaima K Nitta M Shibata F Nakagawa H 《Journal of agricultural and food chemistry》2004,52(14):4571-4576
The effect of (-)-epigallocatechin 3-gallate (EGCG), a major polyphenol of green tea, on neutrophil migration has been studied using multiwell-type Boyden chambers in vitro and a fluorescein isothiocyanate-labeled ovalbumin (FITC-OVA)-induced rat allergic inflammation model in vivo. EGCG inhibited rat neutrophil chemotaxis toward cytokine-induced neutrophil chemoattractant-1 (CINC-1) in a concentration-dependent manner. In addition, CINC-1-induced neutrophil chemotaxis was suppressed by the pretreatment of rat neutrophils with EGCG at the concentration over 15 microg/mL. EGCG caused concentration-dependent suppression of the transient increase in CINC-1-induced intracellular free calcium level in both rat neutrophils and rat CXC chemokine receptor 2 (CXCR2)-transfected HEK 293 cells. EGCG inhibited CINC-1 production by IL-1beta-stimulated rat fibroblasts (NRK-49F cells) and lipopolysaccharide-stimulated rat macrophages at the concentration over 50 microg/mL, a comparatively high concentration. Oral administration of EGCG (1.0 mg or 1.5 mg/rat) at 1 h before the challenge with FITC-OVA suppressed neutrophil infiltration into the air pouch (inflammatory site) in the air-pouch type FITC-OVA-induced allergic inflammation in rats. Chemokine levels in the pouch fluids, however, were not influenced by EGCG administration. The results suggest that EGCG suppressed neutrophil infiltration by a direct action on neutrophils, but not by indirect actions, including the suppression of chemokine production at the inflammatory site. 相似文献
92.
Glaucous leaf and tough rachis phenotypes are rare in Aegilops tauschii, the D genome donor to common wheat (Triticum aestivum). The genes for glaucous leaf and tough rachis were mapped using microsatellite probes in A. tauschii. The glaucous phenotype was suppressed by the inhibitor W2I located on chromosome 2DS. The gene W2I was mapped to the distal part of 2DS, and was unlinked to the centromere. This suggests that the distance of the W2I locus from the centromere was maintained during the evolution of hexaploid wheat from its diploid progenitors as the inhibitor
gene is at the same position in A. tauschii and bread wheat. The Brt (Brittle rachis of A. tauschii) locus was located on the short arm of chromosome 3D, and was 19.7 cM from the centromeric marker, Xgdm72.3D. Brt causes breakage of the spike at the nodes, thus creating barrel-shaped spikelets, while Br1 in hexaploid wheat causes breakage above the junction of the rachilla with the rachis such that a fragment of rachis is attached
below each spikelet. 相似文献
93.
H. Yoshida H. Akimoto M. Yamaguchi M. Shibata Y. Habu S. Iida Y. Ozeki 《Euphytica》2004,135(2):247-253
In vegetatively propagated plants, branches of periclinal chimera give rise to bud mutants, or “sports”, which have been used
to breed new cultivars. Here, we have examined DNA methylation profiles in the cells of the L1 and L2+3 layers from single
plants of vegetatively propagated carnations. The band patterns of methylation-sensitive amplified fragment polymorphism (M-AFLP)
of the genomic DNAs in the L1 and L2+3 layers of the carnation cultivar, “White Sim”, vegetatively propagated over the past
50 years, were completely different. The bud mutant, “White Mind”, obtained from “White Sim” about 25 years ago, also had
very different M-AFLP patterns to the parental “White Sim” line. The cultivar, “Red”, which was separated from “Satisfaction”
as a bud mutant two years ago, showed similar patterns to “Satisfaction” in each corresponding layer. However, we were able
to detect minor, but significantly different M-AFLP patterns between the cultivars, “Red” and “Satisfaction”. The number of
bands that differed between these cultivars was larger in the L2+3 layers than in the L1 layers. The results indicate that
the DNA methylation profiles differ between each cell layer derived from distinct cell lineages of vegetatively propagated
plants, and that changes in these profiles occur frequently and accumulate in the cells of the L2+3 layers, rather than in
the L1 layer.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
94.
Kanokrat Limpisophon Junichirou Shibata Yuki Yasuda Munehiko Tanaka 《Journal Of Aquatic Food Product Technology》2020,29(8):736-749
ABSTRACT The effect of Alcalase hydrolysis conditions on antioxidant activities of shark skin gelatin hydrolysate (SSGH) byproduct was studied. Optimal hydrolysis condition for SSGH at 60°C, pH 7.5, and 2.70 AU kg?1 gelatin protein was used for varied hydrolysis times (10–90 min). The IC50 against DPPH radicals of SSGH hydrolyzed for 90 min (SSGH-90) was 27.39 mg ml?1, which is greater than ascorbic acid. SSGH-90 was predominantly composed of 15–20 kDa protein fragments that regulated increases in peroxide value and thiobarbituric acid reactive substances values and suppressed decrease in DHA of a fish oil-in-water emulsion during storage at 30°C for 7 days. 相似文献
95.
Michiyasu Yoshikuni Naoki Shibata Yoshitaka Nagahama 《Fish physiology and biochemistry》1993,11(1-6):15-24
Specific binding of [3H]17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP) to plasma membranes prepared from defolliculated oocytes of rainbow trout
(Onchorhynchus mykiss) was identified and characterized. Binding was rapid and reached equilibrium in 30 min. 17α,20β-DP strongly inhibited [3H] 17α,20β-DP binding in a competitive manner. Scatchard analysis revealed two different binding sites: a high affinity binding
site with a Kd of 18 nM and a Bmax of 0.2 pmoles/mg protein; and a low affinity binding site with a Kd of 0.5 μM and a Bmax of 1 pmoles/mg protein. This binding activity was successfully solubilized with n-heptyl-β-D-thioglucoside. [3H]17α,20β-DP binding to solubilized preparations reached equilibrium in 1h, and was competitively inhibited with 17α,20β-DP
and 17α,20β,21-trihydroxy-4-pregnen-3-one. However, Scatchard analysis showed a single binding site with a Kd of 0.3 μM. The reason for the disappearance of the high affinity binding site in solubilized preparations remains unclear.
These results demonstrate that a specific binding site for 17α,20β-DP exists in the plasma membrane of rainbow trout oocytes.
Résumé Une liaison spécifique de le [3H]17α,20β-dihydroxy-4-pregnen-3-one (17α,20β-DP), avec des membranes plasmiques d'ovocytes défollicularisés de truite arc-en-ciel (Onchorhynchus mykiss), a été identifiée et caractérisée. Sa cinétique est rapide et atteint son équilibre en 30 minutes. Le 17α,20β-DP inhibe fortement, et de manière compétitive, la liaison de la [3H] 17α,20β-DP. Une étude de Scatchard a mis en évidence deux sites diffŕents de liaison: un site de forte affinité, de Kd 18 nM et de Bmax 0,2 pmoles/mg de protéine; et un site de faible affinité, de Kd 0,5 μM et de Bmax 1 pmoles/mg de protéine. L'activité de liaison a été solubilisée, avec succés, par le n-heptyl-β-D-thioglucoside. Dans la fraction soluble, la liaison de le [3H]17α,20β-DP atteint un équilibre en 1h.; et elle est complétement inhibiée par la 17α,20β-DP et le 17α,20β,21-trihydroxy-4-pregnen-3-one. Cependant, une étude de Scatchard ne permet de déceler qu'un seul site de liaison, de Kd 0,3 μM. La disparition du site de liaison de forte affinité dans la fraction soluble reste inexpliquée. Ces résultats démontrent l'existence d'un site spécifique de liaison du 17α,20β-DP dans les membranes plasmiques des ovocytes de truite arc-en-ciel.相似文献
96.
97.
K Sugimoto N Sakurai M Kaneko H Shirasawa K Shibata M Miyata T Noguchi K Uematsu K Shimoda J Sakata 《American journal of veterinary research》1991,52(1):157-163
Use of microangiography is now essential for the study of microcirculation in various organs. Renal microangiographic studies have been reported in rats, rabbits, dogs, human beings, and mice. However, we could not find any report on use of the technique in cattle, despite high incidence of renal disease in that species. The perfusion technique used in mice was improved over that of our previous report, and was applied to normal and diseased bovine kidneys. For the microangiographic technique, composition of the contrast medium, pressure of the injection, duration of perfusion, and washing of kidneys with heparinized saline solution before perfusion are important. In cattle, 1- to 2-mm-thick sections of the kidneys were generally necessary to observe renal vasculature: arcuate and interlobular arteries, afferent arterioles, and glomerular capillaries. In normal bovine kidneys, the angiographic and microangiographic findings were easily recognized as normal, compared with those of normal mice. In affected bovine kidneys, which histologically represented glomerulonephritis and pyelonephritis, angiography and microangiography revealed corresponding findings. 相似文献
98.
99.
100.
Yasuda K Hashikawa S Sakamoto H Tomita Y Shibata S Fukata T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):205-208
To evaluate the effects of a new synbiotic consisting of Lactobacillus casei subsp. casei (Lcc) and dextran (Dex) on milk production, a total of 58 Holstein dairy cows, which became pregnant and gave birth to calves at regular intervals and lactated steadily and continuously, were selected. The study had a completely randomized design, and the animals were divided into two groups. Group A was fed with a basic diet only, and Group B was fed with a basic diet supplemented with the synbiotic consisting of freeze-dried Lcc and mixed feed containing Dex for one year from August 2004. After supplementation with the synbiotic, milk yields and components of Group B were compared with those of Group A in the August, December of 2004, April and August of 2005. Milk yields of Group B were greater than those of Group A. There were significant differences (p<0.01 or 0.05) between these groups for all values. Furthermore, total amounts of fat, protein and solid non-fat in Group B significantly increased in comparison with those of Group A. In addition, the somatic cell counts of Group A significantly increased in August of 2004 and 2005 in comparison with those of Group B. Thus, the new synbiotic consisting of Lcc and Dex can increase the milk production of Holstein dairy cows throughout the year. 相似文献