A review of advanced techniques for detecting plant diseases

Diseases in plants cause major production and economic losses in agricultural industry worldwide. Monitoring of health and detection of diseases in plants and trees is critical for sustainable agriculture. To the best of our knowledge, there is no sensor commercially available for real-time assessment of health conditions in trees. Currently, scouting is most widely used mechanism for monitoring stress in trees, which is an expensive, labor-intensive, and time-consuming process. Molecular techniques such as polymerase chain reaction are used for the identification of plant diseases that require detailed sampling and processing procedure. Early information on crop health and disease detection can facilitate the control of diseases through proper management strategies such as vector control through pesticide applications, fungicide applications, and disease-specific chemical applications; and can improve productivity.The present review recognizes the need for developing a rapid, cost-effective, and reliable health-monitoring sensor that would facilitate advancements in agriculture. It describes the currently used technologies that can be used for developing a ground-based sensor system to assist in monitoring health and diseases in plants under field conditions. These technologies include spectroscopic and imaging-based, and volatile profiling-based plant disease detection methods. The paper compares the benefits and limitations of these potential methods.     

Chemical control of weeds in wheat (Triticum aestivum L.) in Iran

Experiments were conducted in 2006–2007 at six research stations of the Iranian Plant Protection Research Institute to study the efficacy of different herbicides to control weeds in wheat. Treatments included mesosulfuron-methyl plus iodosulfuron-methyl-sodium plus mefenpyr-diethyl (WG) at 45 + 45 + 135 g a.i./ha, respectively, sulfosulfuron at 21, 31.5, 42 and 51 g a.i./ha, chlorsulfuron at 15 g a.i./ha, bromoxynil plus MCPA at 600 g a.i./ha with clodinafop propargyl at 64 g a.i./ha, sulfosulfuron plus metsulfuron-methyl at 36 g a.i./ha, mesosulfuron-methyl plus iodosulfuron-methyl-sodium plus mefenpyr-diethyl (OD) at 15 + 3 + 45 g a.i./ha, respectively, and a full season weed-free control. Herbicides were applied at wheat tillering. Results showed that sulfosulfuron plus metsulfuron-methyl, and bromoxynil plus MCPA with clodinafop propargyl resulted in satisfactory weed control and wheat grain yield at most locations. Weed control efficacy of both formulations of mesosulfuron-methyl plus iodosulfuron-methyl-sodium was variable across locations. Efficacy of the OD formulation appears to depend upon location, so that application of this herbicide at Shiraz and Gorgan resulted in better weed control compared to use of the WG. Satisfactory performance of the OD formulation at Gorgan and Shiraz could be attributed in part to the even pattern of rainfall distribution during the growing season and wheat cultivar used, respectively. With respect to grain yield, however, the OD formulation was better than WG formulation at most of the locations.     

Molecular characterisation of <Emphasis Type="Italic">Turnip mosaic virus</Emphasis> isolates from Brassicaceae weeds

Eight provinces of Iran were surveyed during 2003–2008 to find Brassicaceae reservoir weed hosts of Turnip mosaic virus (TuMV). A total of 532 weed samples were collected from plants with virus-like symptoms. The samples were tested for the presence of TuMV by enzyme-linked immunosorbent assay using specific antibodies. Among those tested, 340 samples (64%) were found to be infected with TuMV. Rapistrum rugosum, Sisymberium loeselii, S. irio and Hirschfeldia incana were identified as the Brassicaceae weed hosts of TuMV, and the former two plant species were found to be the most important weed hosts for the virus in Iran. The full-length sequences of the genomic RNAs of IRN TRa6 and IRN SS5 isolates from R. rugosum and S. loeselii were determined. No evidence of recombination was found in both isolates using different recombination-detecting programmes. Phylogenetic analyses of the weed isolates with representative isolates from the world showed that the IRN TRa6 and IRN SS5 isolates fell into an ancestral basal-Brassica group. This study shows for the first time the wide distribution and phylogenetic relationships of TuMV from weeds in the mid-Eurasia of Iran.     

Gastrointestinal complaints in shift-working and day-working nurses in Iran

Background

There is evidence in the scientific literature of the adverse physiological and psychological effects of shift work. The work of nurses in hospitals is connected with shift and night work. Several publications have described gastrointestinal disturbances in shift workers. The aim of this study was to compare the frequency of gastrointestinal (GI) complaints of nurses on a rotating shift with that of nurses on a regular day shift.

Methods

The study involved 160 nurses (133 working in shifts and at night and 27 working on day shifts) in the Shahid Beheshti Hospital in Kashan, Iran. These nurses answered a Gastrointestinal Symptom Questionnaire regarding the presence of gastrointestinal symptoms (including heartburn, regurgitation, constipation, diarrhea and bloating). Positive responses required frequent symptom occurrence in the past 4 weeks. Significance of group differences was assessed by chi-square and Fisher-exact tests.

Results

Prevalence of GI symptoms was significantly higher (p = 0.009) in rotating-shift nurses (81.9%) than in day-shift nurses (59.2%). Irregular meal consumption (p = 0.01) and GI medications (p = 0.002) were all significantly higher among the rotating shift nurses. In both groups, regurgitation was the most common symptom.

Conclusion

Nurses on rotating shifts in Iran experience more GI disturbances than do nurses on day shifts.     

A review on hyperthyroidism: thyrotoxicosis under surveillance

Thyrotoxicosis exhibit collective clinical manifestation, caused by excessive serum thyroid hormones particularity thyroxin. The clinical signs and symptoms included general alteration of metabolic process leading to weight loss fatigue and weakness and some specific disorders such as cardiovascular, neuromuscular reproductive gastrointestinal dermatological and bone disorders. The diagnosis of thyrotoxicosis relay on the thyroid function test carried out by the laboratory serum measurement of thyroxin, triiodothyronine and thyroid stimulating hormones accompanied by other para-medical examinations suggested by clinicians and endociologicst. In thyrotoxicosis serum level of thyroid hormones and thyroxin in particular elevated accompanied by pituitary thyroid stimulating hormone suppression reaching to undetectable level in sever thyrotoxicosis. Among the most common cause of thyrotoxicosis are, thyroid autoimmunity diseases thyroid toxic, adenoma toxic nodular and multinodular hyperthyroidism. The main aim behind this review is to explore the clinical manifestation, the causative factors, diagnosis, metabolic disorder occur due to thyrotoxicosis.     

Protochlorophyllide spectral forms

Abstract: This study summarized recent results on POR and plastid development in order to find an explanation for the existence of Pchlide spectral forms. This review has summarized many researches about the Pchlide spectral forms and their phototransformability in different higher plants which have been published. Chlorophyll (Chl) is the most important pigment on the Earth. Each spring millions of tons Chl are formed during bud break and leaf development. The sun light needed for photosynthesis is captured by Chl and transformed to chemical energy. In the Biosynthesis of chlorophyll (Chl) begins with the synthesis of delta-aminolevulinic acid (ALA) from glutamic acid. Chl biosynthetic pathway, a light dependent enzyme protochlorophyllide oxidoreductase (POR) catalyses a key light-driven reaction, trans addition of hydrogen across the C-17-C-18 double bond of the Chl precursor, protochlorophyllide (Pchlide), that triggers a profound transformation in plant development. Pchlide is spectrally heterogeneous and exist in different spectral forms having slightly different absorption and fluorescence peaks. The identified Pchlide spectral forms can be sorted into three groups. The first group is designated as short-wavelength forms. This group with fluorescence in the 625-646 nm spectral region has a heterogeneous nature and is made by four components. The second group includes the long-wavelength Pchlide forms with emission maxima between 652 and 657 nm. The third group is found in the extreme red region (670-730 nm) of the fluorescence emission spectra and includes a number of pigment forms with spectral bands of low intensity. This region is also influenced by vibrational bands of the short-and long-wavelength Pchlide forms.     

Analysis of genotype-by-environment interaction for grain yield of rainfed durum wheat genotypes in warm winter areas of Iran

This study was performed for pattern analysis of genotype-by-environment (GE) interaction on 20 durum wheat genotypes grown in 15 testing environments during 2004–06 in Iran. Combined analysis of variance showed significant genotypes (G), environments (E), and GE interactions (P < 0.01), with environmental main effects being the predominant source of variation, followed by GE interaction. The results showed various patterns of genotype responses to different environment groups and assisted in structuring the durum wheat testing locations with identification of two major-environment groups with high genotype discrimination ability. The locations (Gachsaran and Ilam) corresponding to warm and semi-arid aresa were similar in genotype discrimination and showed no association with the other testing locations (Gonbad, Moghan, and Khoramabad) representing the Mediterranean area, indicating they differ in rankings of genotypes. The top-yielding genotypes, G13, G14 and G9, were highly adapted to warm and semi-arid environments, but those corresponding to the Mediterranean area had a high ability to discriminate the genotypes G16, G11, and Saimareh. The stability and adaptability of specific genotypes were assessed by plotting their nominal grain yields at specific environments in an ordination biplot, which aided in the identification of environment groups. Appropriate check genotypes for all environments or for specific environments were also identified. Pattern analysis allowed a sensible and useful summarization of GE interaction data set and helped to facilitate selecting superior genotypes for target-growing sites.     

Tree-Temperature Monitoring for Frost Protection of Orchards in Semi-Arid Regions Using Sprinkler Irrigation

Automated over-tree sprinkler irrigation systems were developed and tested in two orchards located in two separate locations in southern Iran （a 0.17 ha peach orchard and a 0.24 ha orange orchard） to protect peach blossoms and orange trees leaves and fruits from low temperature damage. The experiment used a system that monitored the trees and air temperatures using two thermistors. The water application rate by the irrigation system was determined by an energy balance as implemented by the software FROSTPRO, In the peach orchard, the system was tested during three frost events during the spring of 2003 and three other events during the spring of 2004. The system successfully kept peach flowers above the critical temperature, i.e., -4.0℃ in spring 2004 （control block -4.12℃, and sprinkled block ＋0.5℃） during all events. Similar results were obtained in the orange orchard during three frost events in the winter of 2004, during which the tree temperatures were at least 2.5℃ above the critical temperature. Results from field tests show that the system can effectively protect the peach blossoms from damage. Determination done after the frost events showed a 12% blossom kill in the sprinkled blocks while in the unsprinkled control block a 41.5% blossom kill. Calculations indicated that when using variable application rates, the amount of water used can be reduced by 54.3%. Spatial distribution of minimum temperatures during the three frosts was also studied in Jahrom, Iran. Results showed a significant temperature control in the experimental block, especially in the central part of the orchard, but the block margins （about 3.6% of the total area） were at the risk of low temperature due to the wind drift effects.     

Assessment of different anther culture approaches to produce doubled haploids in cucumber (<Emphasis Type="Italic">Cucumis sativus</Emphasis> L.)

Cucumber is one of the most important vegetable crops worldwide, which makes it a good candidate to produce doubled haploid (DH) lines to accelerate plant breeding. Traditionally, these approaches involved induction of gynogenesis or parthenogenesis with irradiated pollen, which carries some disadvantages compared to androgenesis. Despite this, studies on anther/microspore cultures in cucumber are surprisingly scarce. Furthermore, most of them failed to unambiguously demonstrate the haploid origin of the individuals obtained. In this work we focused on anther cultures using two cucumber genotypes, different previously published protocols for anther culture, different in vitro culture variants to make it more efficient, and most importantly, a combination of flow cytometry and microsatellite molecular markers to evaluate the real androgenic potential and the impact of anther wall tissue proliferation. We developed a method to produce DH plants involving a bud pretreatment at 4 °C, a 35 °C treatment to anthers, culture with BAP and 2,4-D, and induction of callus morphogenesis by an additional 35 °C treatment and sequential culture first in liquid medium in darkness and second in solid medium with light. We also found that factors such as genotype, proliferation of anther wall tissues, orientation of anthers in the culture medium and growth regulator composition of the initial anther culture medium have a remarkable impact. Our rate of chromosome doubling (81%) was high enough to exclude additional chromosome doubling steps. Together, our results present androgenesis as an improvable but yet more convenient alternative to traditional gynogenesis and parthenogenesis-based approaches.