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排序方式: 共有196条查询结果,搜索用时 234 毫秒
61.
62.
OBJECTIVE: To describe a technique for laparoscopic cryptorchidectomy in standing horses using electrosurgical instrumentation. STUDY DESIGN: Retrospective clinical study. ANIMALS OR SAMPLE POPULATION: Ten horses, 1 to 7 years of age, with unilaterally or bilaterally retained testes. METHODS: Food was withheld for a minimum of 12 to 24 hours. Horses were sedated using xylazine hydrochloride (0.5 to 1 mg/kg) and butorphanol tartrate (0.02 mg/kg) or detomidine hydrochloride (0.02 to 0.03 mg/kg) and restrained in standing stocks. Three portal sites in the paralumbar fossae were locally desensitized using 2% mepivacaine. After trocar and laparoscope insertion, the ipsilateral testicle, mesorchium, and ductus deferens were identified. The cranial mesorchium was coagulated with either monopolar (one horse) or bipolar (nine horses) electrosurgical forceps, and then the mesorchium, ductus deferens, and ligament of the tail of the epididymis were transected from cranial to caudal using laparoscopic scissors. Once the testis was freed, the transected mesorchium was inspected for hemorrhage and the testis was removed by connecting the two instrument portals (eight horses). In two horses, the testis was placed within a laparoscopic retrieval bag and then removed without enlarging the portal incision. If the testes were retained bilaterally, the retained contralateral testis was removed similarly through the opposite paralumbar fossa. If the contralateral testis was descended, it was removed by a standard, standing castration technique. RESULTS: Vessels of the mesorchium were adequately coagulated using bipolar and monopolar electrosurgical forceps. No immediate or short-term complications occurred in 10 horses at 3 to 11 months after surgery. CONCLUSION: Standing laparoscopic cryptorchidectomy can be performed easily and safely using electrosurgical instrumentation as the sole means of providing hemostasis of the equine mesorchium. CLINICAL RELEVANCE: Standing laparoscopic cryptorchidectomy using electrosurgical instrumentation provides a safe, reliable, and efficient alternative to achieve hemostasis of the equine mesorchium. 相似文献
63.
Babesia divergens was cultivated in sheep erythrocytes in RPMI 1640 supplemented with 10% Fetal Calf Serum (FCS) or sheep serum. In vitro cultures in sheep red blood cells were initiated with human erythrocytes infected in vitro with B. divergens Rouen 1987 or with gerbil blood infected with several isolates from bovine origin. After the first subcultures on sheep erythrocytes, a ten-fold multiplication of the parasites was obtained within 48 h. Erythrocytes from three splenectomized sheep were infected in vitro with B. divergens; when parasitaemia reached 10%, the animals were inoculated with homologous parasitized erythrocytes. All sheep expressed hyperthermia with a peak between the 6th and the 9th day post-infection (p-i) and a transitory parasitaemia 10 days p-i. In vitro primary cultures were performed on two of these sheep, demonstrating the parasite persistence at very low parasitaemia in the infected animals. Splenectomized sheep can be used as a new model for B. divergens chronic infection. 相似文献
64.
Monique Wyers J. C. Hamon Y. Cherel G. Plassiart 《Anatomia, histologia, embryologia》1993,22(1):48-58
The comparative cortical growth of the tibio-tarsus was studied during the first six weeks after hatching in two turkey strains, a light and a heavy one. At one day post-hatching the cortical appeared to be constituted in both strains of an embryonary non-active fibrous bone tissue which had completely disappeared at one week. On and after one week the cortice was constituted of a fibrolamellar adult bone tissue elaborated on the periosteal part of the cortex. Its vascular pattern depended on the strain and on the thickness of the cortical faces. The histomorphometrical studies showed that the growth in thickness was earlier and faster in the heavy strain with a Total Diaphyseal Surface twice larger at 6 weeks but a Diaphyseal Cortical Ratio not significantly different since 3 weeks of age. 相似文献
65.
Delphine Bibbal Monique Kérourédan Estelle Loukiadis Flemming Scheutz Eric Oswald Hubert Brugère 《Veterinary microbiology》2014,168(2-4):451-454
Enteroaggregative Shiga-toxin-producing Escherichia coli strains were responsible for a massive outbreak in Europe in 2011, and had been previously isolated from French patients. The objective of this study was to investigate the presence of enteroaggregative E. coli (EAEC) in slaughterhouse effluents (wastewater, slurry, sludge and effluents), and in river waters near these slaughterhouses. A total of 10,618 E. coli isolates were screened by PCR for the presence of EAEC-associated genetic markers (aggR, aap and aatA). None of these markers was detected in E. coli isolated from slaughterhouse samples. A unique enteroaggregative E. coli (EAEC) O126:H8 was detected in river water sampled upstream from slaughterhouse effluent discharge. These results confirmed that animals might not be reservoirs of EAEC, and that further studies are required to evaluate the role of the environment in the transmission of EAEC to humans. 相似文献
66.
Ligia Ayala Monique Henry Maarten van Ginkel Ravi Singh Beat Keller Mireille Khairallah 《Euphytica》2002,128(2):249-259
We searched for QTLs involved in tolerance to barley yellow dwarf (BYD), a serious viral disease of small grain cereals in
two wheat populations, Opata × Synthetic (ITMI)and Frontana × INIA66 (F × I), for which marker data had previously been generated.
The populations were evaluated in replicated field trials under artificial inoculation with a BYDV-PAV-Mex isolate and under
disease-free conditions. Disease symptoms (yellowing, dwarfism and biomass reduction) were visually recorded and agronomic
traits (number of tillers,height, biomass, yield and thousand-kernel weight) were measured on five plants per plot. Phenotypic
data on all evaluated traits showed normal distribution with high correlation between visual estimates and measured values.
Heritabilities were mostly moderate to high in the 114 lines of the ITMI population, and from low to moderate in the 117 lines
of the F × I population. QTL analyses were based on genetic maps containing 443 loci for the ITMI population and 317 loci
for the F × I population. Using composite interval mapping, 22 QTLs in the ITMI population and seven in the F × I population
were detected, explaining9.8–43.3% of total phenotypic variation (σ2
P)per agronomic trait in the first population, and 4.1–13.7% in the second. Individual QTLs explained less than 15.8%of σ2
P. In the F × I population a minor QTL explaining 7% of σ2
P for yellowing was detected on the short arm of 7D, linked to leaf tip necrosis, a morphological marker for linked genes Bdv1, Yr18 andLr34. A QTL consistently detected for several traits on 2D in the ITMI population and on the short arm of group 6 chromosome(6S)
in F × I explained 10–15% of σ2
P. The large number of QTLs having mostly small effects and the continuous distribution of all evaluated traits confirmed the
polygenic nature and complexity of BYD tolerance in wheat.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
67.
Hélène Laparra Monique Burrus Reiner Hunold Brigitte Damm Ana Maria Bravo-Angel Roberte Bronner Günther Hahne 《Euphytica》1955,85(1-3):63-74
Summary Suitable sunflower tissues and cells were transformed either by direct gene transfer into protoplasts, particle bombardment, or Agrobacterium co-culture. While all techniques allowed efficient short-term or transient expression of the introduced gene(s) in the respective tissues, stable transformation was only observed after transformation with Agrobacterium. The latter technique was suitable for the production of transgenic callus from seedling cotyledons and occasional shoots with chimaeric expression of the transgene. Detailed analysis of the interaction of Agrobacterium with this explant showed that infection efficiency was critically dependent on the co-culture conditions, and that the preferentially-transformed cells were not the ones competent for regeneration.Abbreviations BAP
benzyl adenine
- CAT
chloramphenicol acetyl transferase
- 2,4-D
2,4-dichloro phenoxy acetic acid
- GUS-
D-glucuronidase
- MS
medium according to Murashige & Skoog (1962)
- NAA
naphthalene acetic acid
- NPTII
neomycin phospho transferase II
- PEG
polyethylene glycol
- PIG
particle inflow gun
- SH
medium according to Schenk & Hildebrandt (1972) 相似文献
68.
69.
Johann Mourier Marc Soria Antonin Blaison Monique Simier Grégoire Certain Angélique Demichelis Tarek Hattab 《水产资源保护:海洋与淡水生态系统》2021,31(10):2926-2937
- Knowledge about spatial and temporal variability in the distribution and abundance of predators is necessary to adapt measures to mitigate human–wildlife interactions.
- Acoustic telemetry and network analyses were used to investigate the spatial ecology of bull sharks, the species responsible for most shark bites in Reunion Island, one of the world's shark bite hotspots.
- The west coast of the island was not used uniformly by every individual, with size predicting the movements of sharks along the coast.
- Node-based metrics – closeness, node strength, and cumulated continuous residency times – derived from up to 181 monthly movement networks from 20 individuals, revealed that smaller sharks (<250 cm total length) primarily used the south-west coast while larger individuals spent most of their time in the northern region with regular visits to multiple areas along the coast.
- This study provides essential knowledge on bull shark behaviour and central areas used at different periods of the year, which correlates well with the dynamics of observed shark bites. Our approach provides a non-invasive alternative to help predicting and anticipating human–shark conflicts and avoid shark culling programmes detrimental to the conservation of large predators such as sharks.
70.
Soyasaponins are phytochemicals of major interest for health. Their identification and quantification remain difficult owing to the large number of structural isomers in soybeans and the lack of stable standards. In this study, a rapid method using high performance liquid chromatography (HPLC) using a UV detector (205 nm) was developed to identify and quantify soyasaponins belonging to group B and compare them with isoflavones in different soy materials. 2,3-Dihydro-2,5-dihydroxy-6-methyl-4H-pyran-4-one (DDMP)-conjugated soyasaponins were determined using external calibration or a molecular mass ratio after alkaline hydrolysis to cleave their DDMP moieties. The detection limit of soyasaponin I, used as a reference molecule to simplify the analysis, was 0.065 micromol/g. Soyasaponin contents in seven soybean varieties ranged from 13.20 to 42.40 micromol/g in the germ and from 2.76 to 6.43 micromol/g in the cotyledons. The within-day and between-days variation coefficients did not exceed 7.9 and 9.0%, respectively, for the major soyasaponins. Soyasaponin B quantification in different soy-based health supplements was reported along with measurements of their isoflavone content to provide information on the variability of these bioactive compounds among different types of soy food materials. 相似文献