In this research, the mineral and organic composition of the seminal plasma, physical spermatological parameters and their physiological relationships were investigated in grass carp (
Ctenopharyngodon idella). The seminal plasma contained 98.14±5.23 mM L
?1 (Na
+), 380.85±25.95 mM L
?1 (K
+), 30.25±4.96 mg dL
?1 (Ca
2+), 19.16±1.70 mEq L
?1 (Mg
2+), 1.36±0.11 mg dL
?1 glucose, 0.37±0.08 g dL
?1 total protein, 12.02±1.18 mg dL
?1 cholesterol, 14.85±1.50 mg dL
?1 triglyceride and 43.5±9.56 mg dL
?1 urea. The following spermatological parameters were found: sperm volume 14.44±1.16 mL, sperm motility 80.60±1.55%, movement duration 67.68±4.32 s, density 15.43±0.72 × 10
9 mL
?1, total density 337.43+45.86 × 10
9 and pH 7.24±0.17. The Na
+ and Ca
2+ ions correlated negatively with spermatozoa motility (
r=?0.453,
P>0.05 and
r=?0.192,
P>0.05) respectively. The K
+ ion correlated positively with spermatozoa motility (
r=0.545,
P>0.05). But a statistically significant correlation was not observed between sperm motility and seminal plasma parameters. The following correlations were observed between mineral and organic components. The Mg
2+ was positively correlated with glucose and cholesterol (
r=0.692,
P<0.05 and
r=0.680,
P<0.05) respectively. A highly significant positive relationship was also found between Mg
2+ and total protein (
r=0.837,
P<0.01). On the other hand, a significantly negative relationship was found between Ca
2+ and triglyceride (
r=?0.639,
P<0.05). These parameters should be considered when developing procedures for either artificial fertilization or for cryopreservation of grass carp sperm.
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