The pathology of Johne''s disease in sheep

The clinical, gross and histopathological findings in 50 sheep affected with Johne's disease are described. Clinically 90% were emaciated and 20% showed severe diarrhoea. On necropsy there was thickening of the walls of the intestines, particularly of the ileum, caecum and less frequently the jejunum, but in 36% of sheep the changes were only mild. Histologically there was a granulomatous enteritis, typhlitis and colitis, with the most severe changes in the terminal ileum. High numbers of acid-fast organisms were present in the terminal ileum in over 70% of sheep. Mycobacterium paratuberculosis was cultured from only 8% of the sheep examined.     

Serum chemistry alterations in Alaskan sled dogs during five successive days of prolonged endurance exercise

OBJECTIVE: To determine the impact of successive days of endurance exercise on select serum chemistry values in conditioned Alaskan sled dogs. DESIGN: Prospective cohort study. ANIMALS: 10 conditioned Alaskan sled dogs. PROCEDURES: All dogs ran 160 km/d for 5 consecutive days. Serum was obtained prior to exercise and immediately after each exercise run; all samples were obtained before dogs were fed. Serum electrolyte, mineral, protein, total bilirubin, urea nitrogen, creatinine, and cardiac troponin-I concentrations and serum alkaline phosphatase, alanine aminotransferase, creatine kinase, and aspartate aminotransferase activities were measured. Data were analyzed by means of analysis of covariance for a randomized complete block design with dog as a blocking variable, time as a covariate, and distance run as the treatment of interest. Least square mean values were compared with values obtained prior to exercise, and linear and quadratic contrasts were examined. RESULTS: Serum globulin concentration was low prior to exercise (mean +/- SD, 2.2 +/- 0.3g/dL) and progressively decreased as exercise continued. Exercise was associated with increases in serum chloride, urea nitrogen, and cardiac troponin-I concentrations and serum alanine aminotransferase, creatine kinase, and aspartate aminotransferase activities and with progressive decreases in serum potassium, total protein, and albumin concentrations. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that multiple successive days of endurance exercise resulted in mild aberrations in serum chemistry variables in conditioned sled dogs. Changes likely reflected the metabolic stresses of prolonged endurance exercise as well as dietary composition. Hypoglobulinemia in resting, conditioned sled dogs may reflect the immunosuppressive or catabolic effects of intense endurance training.     

Evidence from tag recapture experiments that fish learn to avoid fishing gear

We found evidence that tagging induced trap shyness in snapper (Pagrus auratus), i.e., tagged fish had a reduced probability of recapture by the method by which they had originally been caught. Tagging experiments in 1985 and 1994 involving single release over a short period and single recapture were conducted on a closed population (SNA 1: East Northland-Hauraki Gulf-Bay of Plenty). Initial capture was by trawling and by line fishing, while recapture over an extended period included other methods. A test for trap shyness that removed the possible effects of spatial and fish size heterogeneity gave a significant result for both years. The data suggested that the trap shyness effect might have been smaller for trawl-released fish than for line-released fish. However, we estimated a single trap shyness factor (0.71). There was also some evidence for attenuation of trap shyness over time.     

Selecting representative reserve systems in remote areas: A case study in the Nullarbor region, Australia

We present a procedure for the selection of a network of reserves representing the biological diversity of a large and biologically poorly known region. The quantitative analysis gives equal weighting to a wide array of different species: mobile and sessile, long-lived and ephemeral, heterothermic and homeothermic, etc.

Sampling was based on quadrats that were positioned through the region using a stratified random strategy. This provided a presence-absence matrix of the species composition at each quadrat. Numerical pattern analysis was used to identify 14 species assemblages and to re-order the sites and species in the data matrix. The geographic pattern of each assemblage throughout the region was described by contouring assemblage richness, the isolines being the proportion of the number of species in each assemblage. The data matrix was re-examined. Some assemblages exhibit several gradients in species composition. For example, a 0·1 isoline in the east may represent a different 10% of species than a 0·1 isoline in the west. Other assemblages exhibit only a single gradient in species composition.

The results were used to select the optimum positions of reserves needed to represent the compositional diversity of each of the 14 species assemblages.

Limited field checking confirmed the predicted isolines in assemblage compositional richness to a satisfactory extent.     

Crop and soil response to subsoil loosening, deep incorporation of phosphorus and potassium fertilizer and subsequent soil management on a range of soil types.: Part 2: Soil structural conditions

Abstract. In a series of experiments on 16 sites both a power-driven rotary-tine Wye Double Digger and a rigid tine winged subsoiler produced significant subsoil loosening and fissuring. The Double Digger consistently produced the greatest clod breakdown together with the least soil bulk densities and cone penetration resistances. Management strategies after loosening had an important influence on the longevity of the loosening effect. The rate of recompaction was least with controlled traffic and bed systems and increased with random traffic and with the growing of root crops. No significant differences in crop response were monitored between the two loosening treatments, yield response depending largely on the extent of moisture stress experienced by the crop. Loosening on silty soils reduced yields in wet seasons and this was associated with soil structural instability. Visual soil profile examination is necessary to support bulk density and cone penetration resistance measurements when assessing soil compaction.     

Nutritional and physiological responses of young growing rats to diets containing raw cowpea seed meal,protein isolate (globulins), or starch

The nutritional and physiological effects of raw cowpea (Vigna unguiculata (L) Walp.) seed meal, protein isolate (globulins), or starch on the metabolism of young growing rats have been evaluated in 14-day trials. Wet and dry weight gain, feed conversion efficiency, and lipid and protein accretion were significantly reduced as a result of inclusion of seed meal, globulins, or starch in the diet, with growth retardation being most marked with the seed meal. The proportional weights of the small intestine and pancreas were increased by meal diets, and serum cholesterol levels were slightly reduced. The globulins and raw starch also increased relative small intestine weights but had no effect on the pancreas or serum constituents. The effects of cowpeas on rats appeared to be due primarily to the combined actions of globulins, resistant starches, protease inhibitors, and possibly fiber and non-starch polysaccharides on intestinal and systemic metabolism.     

Amelioration of a hardsetting Alfisol through deep mouldboard ploughing, gypsum application and double cropping. I. Soil physical and chemical properties

Contrasting soil management techniques were applied to a hardsetting red-brown earth (Alfisol) used for flood-irrigated wheat (Triticum aestivum) production at Trangie, N.S.W., Australia. The individual and combined effects of deep mouldboard ploughing to a depth of 0.45 m, gypsum application (5 t ha⁻¹) and double cropping upon aggregate stability, bulk density, porosity, cone index and the non-limiting water range were evaluated. Dispersion and slaking of the surface soil were unaffected by the treatments when measured at the end of the second year of the experiment. Approximately 60% of the soil mass in the 0–0.15 m layer slaked on wetting, whereas less than 1% of the soil dispersed. Organic carbon (OC) levels of the surface soil were not affected by double cropping or gypsum application, but were reduced by mouldboard ploughing from 0.9% to 0.6% OC. The relationship between OC and macroaggregate (more than 250 μm) stability indicated that large increases in OC beyond 0.7% OC were required for relatively small increases in aggregate stability. Mouldboard ploughing increased clay content of the upper 0.15 m of the soil from 22% to 27%. This was associated with an increase in the frequency and depth of cracking which, however, diminished over time. The non-limiting water range (NLWR) was expanded in the uppermost 0.1 m by gypsum application from 0.15–0.30 to 0.09–0.28 m³ m⁻³. Mouldboard ploughing expanded the NLWR at a depth of 0.2 m. Penetrometer resistance, on average, exceeded the critical value for wheat root growth at a water content of 0.15 m³ m⁻³, which is substantially higher than the wilting point (0.09 m³ m⁻³). Excessive resistance to penetration as opposed to inadequate aeration or water availability is the main agronomic impediment in these soils, at least in the initial stages of crop development. Penetration resistance within the 0.05–0.3 m layer was reduced during a drying cycle in the order: mouldboard ploughing>gypsum>double cropping. The reduced penetration resistance associated with mouldboard ploughing was due to higher water content to a depth of 0.2 m and reduced bulk density below this depth.     

Amelioration of a hardsetting Alfisol through deep mouldboard ploughing, gypsum application and double cropping. III. Crop production and profitability

Deep mouldoard ploughing to 0.45 m, gypsum application (5 t ha⁻¹), and double cropping were evaluated, alone and combined, as ameliorants for a hardsetting red-brown earth (Alfisol). The double cropped treatment consisted of winter wheat (Triticum aestivum) and a summer forage crop consisting of sudax (Sorghum sudanese) intersown with cowpea (Vigna unguiculata). This paper describes the effect of these treatments on crop development, yield, crop-water relations and economic returns. Crop emergence was not affected by any treatment. Mouldboard ploughing significantly increased wheat grain yield from 1.86 to 2.15 t ha⁻¹, in 1984, but not in 1985. Yield was also increased from 5.18 to 5.68 t ha⁻¹ as a result of gypsum addition in 1985—after no significant difference in 1984. Double cropping significantly increased yield (by 0.6 t ha⁻¹) in 1985. Greater increases were obtained with the summer cropping phase where higher evaporative demand during the summer would have aggravated soil structural problems associated with hardsetting. Sudax-cowpea dry matter yields were increased from 13.6 to 17 t ha⁻¹ and from 13.0 to 17.5 t ha⁻¹ during summer 1984–1985 by gypsum and mouldboard ploughing, respectively. The higher yields are attributed to increased water storage and depletion, and reduced soil strength for the mouldboard ploughed and gypsum main treatments. No interactions were found between the main treatments, except at the end of the 1985 winter cropping phase when there was a negative interaction between mouldboard ploughing and gypsum application for dry matter, equivalent root length and yield. The yield increases associated with mouldboard ploughing did not persist beyond the second year of the experiment. Double cropping combined with mouldboard ploughing (Mb+DC) was the most profitable treatment, whereas mouldboard ploughing with gypsum (Mb+G) was the least profitable for the crops grown in this experiment.     

Inhibition of the complement cascade by the major secretory protein of vaccinia virus

The complement system contributes to host defenses against invasion by infectious agents. A 35-kilodalton protein, encoded by vaccinia virus and secreted from infected cells, has sequence similarities to members of a gene family that includes complement control proteins. Biochemical and genetic studies showed that the viral protein binds to derivatives of the fourth component of complement and inhibits the classical complement cascade, suggesting that it serves as a defense molecule to help the virus evade the consequences of complement activation.     

Venus volcanism: initial analysis from magellan data

Magellan images confirm that volcanism is widespread and has been fimdamentally important in the formation and evolution of the crust of Venus. High-resolution imaging data reveal evidence for intrusion (dike formation and cryptodomes) and extrusion (a wide range of lava flows). Also observed are thousands of small shield volcanoes, larger edifices up to several hundred kilometers in diameter, massive outpourings of lavas, and local pyroclastic deposits. Although most features are consistent with basaltic compositions, a number of large pancake-like domes are morphologically similar to rhyolite-dacite domes on Earth. Flows and sinuous channels with lengths of many hundreds of kilometers suggest that extremely high effusion rates or very fluid magmas (perhaps komatiites) may be present. Volcanism is evident in various tectonic settings (coronae, linear extensional and compressional zones, mountain belts, upland rises, highland plateaus, and tesserae). Volcanic resurfacing rates appear to be low (less than 2 Km(3)/yr) but the significance of dike formation and intrusions, and the mode of crustal formation and loss remain to be established.