全文获取类型
收费全文 | 226篇 |
免费 | 7篇 |
国内免费 | 2篇 |
专业分类
林业 | 39篇 |
农学 | 5篇 |
25篇 | |
综合类 | 6篇 |
农作物 | 7篇 |
水产渔业 | 23篇 |
畜牧兽医 | 118篇 |
园艺 | 2篇 |
植物保护 | 10篇 |
出版年
2021年 | 11篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 2篇 |
2017年 | 5篇 |
2016年 | 7篇 |
2015年 | 15篇 |
2014年 | 20篇 |
2013年 | 22篇 |
2012年 | 11篇 |
2011年 | 17篇 |
2010年 | 9篇 |
2009年 | 13篇 |
2008年 | 16篇 |
2007年 | 11篇 |
2006年 | 17篇 |
2005年 | 11篇 |
2004年 | 11篇 |
2003年 | 7篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1979年 | 2篇 |
1958年 | 1篇 |
排序方式: 共有235条查询结果,搜索用时 203 毫秒
61.
62.
Mariko Shoji Hidenori Sato Remi Nakagawa Ryo Funada Takafumi Kubo Shinjiro Ogita 《Journal of Forest Research》2006,11(6):449-453
The effect of an osmoticum, polyethylene glycol (PEG), on somatic embryo production was examined using embryogenic cells of
Pinus densiflora. In the basal medium containing 30 μM abscisic acid and 6% maltose, the quality of the embryos formed was poor even though
somatic embryos were produced. The addition of PEG with molecular weight of 4000 or 8000 significantly enhanced the development
of both the quality and quantity of somatic embryos. Furthermore, higher levels of a constant osmotic pressure with PEG 8000
in a range from about 300 to 450 mmol/kg could remarkably enhance the morphogenesis of somatic embryos and their number of
embryos produced. A higher stable osmotic pressure with an appropriate molecular weight of PEG is a key factor for the production
of good quality somatic embryos in P. densiflora. 相似文献
63.
Mariko Yoshioka Keiji Takabe Junji Sugiyama Yoshiyuki Nishio 《Journal of Wood Science》2006,52(2):121-127
New biodegradable cellulose acetate/layered silicate grafted poly(ε-caprolactone) [(CA/layered silicate)-g-PCL] nanocomposites were prepared by in situ polymerization of ε-caprolactone in the presence of cellulose acetate (CA) and organically modified layered silicate (OMLS). The structures of
the resulting composites were investigated. X-ray diffractometry was carried out to survey general structural features of
(CA/OMLS)-g-PCL nanocomposites, and revealed that OMLSs having hydroxyl groups in the organic modifiers greatly altered the layered silicate
structure by monomer intercalation and successive exfoliation through its polymerization. Two of the representative cases
were characterized by wide-angle and small-angle X-ray scattering analyses with a synchrotron source. The morphology of these
nanocomposites was further examined by transmission electron microscopy. When SPN, one of OMLSs having one hydroxyl group
in its modifier, was used, the silicate layers could not be dispersed thoroughly, but existed as aggregates consisting of
several silicate layers. Among them, the crystal growth of PCL developed by transcrystallization, where the crystal growth
was restricted in the confined space. When Cloisite 30B, having two hydroxyl groups within the modifier, was used, the silicate
layers forming the clay were dispersed completely in the composite and random orientation of the OMLS was observed. 相似文献
64.
For plywood specimens under shear through the thickness, a fatigue life prediction method based on strain energy has been
newly developed with the fatigue process and failure criterion applicable to various loading conditions. Once the fatigue
process and failure criterion of the plywood specimen were determined by the fatigue data measured under a loading condition
other than the square loading waveform, the fatigue life of a specimen under various loading conditions could be predicted
easily and accurately by the first cycle loading test. The relationship between stress level and the predicted fatigue life
was also similar to that between stress level and the experimentally determined fatigue life. The fatigue life prediction
method proposed may be widely applicable to the prediction of the fatigue life of solid wood and wood composites. 相似文献
65.
Takahama U Tanaka M Oniki T Hirota S Yamauchi R 《Journal of agricultural and food chemistry》2007,55(10):4169-4176
The objective of the present study was to elucidate how chlorogenic acid in coffee was transformed under acidic conditions simulating the mixture of saliva and gastric juice. When coffee was incubated in acidified saliva that contained nitrite and SCN-, in addition to nitric oxide (NO), four major components were detected. Two of the four components (components 3 and 4) were generated when chlorogenic acid was incubated in acidified saliva and when incubated in an acidic buffer solution in the presence of both nitrite and SCN-. By the incubation of chlorogenic acid in acidic nitrite in the absence of SCN-, components 3 and 4 were not formed but the quinone of chlorogenic acid and nitrated chlorogenic acid were formed. The result indicates that SCN- was indispensable for nitrous acid induced formation of components 3 and 4. Component 4 was isolated and its structure was determined to be (E)-5'-(3-(7-hydroxy-2-oxobenzo[d] [1,3]oxathiol-4-yl)acryloyloxy)quinic acid. Component 3, which was suggested to be 2-thiocyanatochlorogenic acid, seemed to be formed by the reaction between SCN- and the quinone of chlorogenic acid. As it has been reported that the quinone of chlorogenic acid can react with thiols and can decompose producing H2O2, the formation of component 4 can reduce the toxic effects of the quinone of chlorogenic acid. 相似文献
66.
Park CH Kondo M Inoue S Noguchi A Oyamada T Yoshikawa H Yamada A 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(6):589-595
A fatal encephalomyelitis was developed after intracerebral and hind limb inoculation of in 6-week-old C57BL/6J mice by the inoculation of fixed rabies virus (CVS-11 strain), intracerebrally and into hind. After the intracerebral inoculation, virus antigens were detected in the cerebral cortex and hippocampus at 2 days postinoculation (PI), and later spread centrifugally to thalamus, brain stem, cerebellum, spinal cord and spinal ganglia. At 4 days PI, severe apoptosis and DNA fragmentation were observed in the hippocampus and cerebral cortex. All mice infected intracerebrally were dead without limb paralysis at from 10 to 11 days PI. In contrast, mice infected with virus intramuscularly were persistently observed virus antigens in the myocytes at the site of inoculation from 2 days PI. At 4 days PI, the antigens were demonstrated in the spinal dorsal root ganglia, spinal cord and muscle spindles without their detection in the cerebrum and hippocampus. There were no apoptosis in the spinal cord and dorsal root ganglia, however hind limb paralysis was found in all infected mice. Hind limb paralysis was progressed to quadriparalysis, and mice were dead from 11 to 13 days PI. From 4 days PI, necrosis of neuron was observed in the the spinal and dorsal ganglia with infiltration of lymphocyte. This study suggested that the necrosis of spinal neurons was more important to cause the paralysis of hind limb rather than the severe cerebral infection and apoptosis in C57BL/6J mice infected with CVS-11 strain. The virus primarily replicated in the muscles was ascended the spinal cord via afferent fibers and retrogradely invaded the cerebrum, and with subsequent spread to muscle spindles. 相似文献
67.
68.
Identification of small molecule activators of cryptochrome 总被引:1,自引:0,他引:1
T Hirota JW Lee PC St John M Sawa K Iwaisako T Noguchi PY Pongsawakul T Sonntag DK Welsh DA Brenner FJ Doyle PG Schultz SA Kay 《Science (New York, N.Y.)》2012,337(6098):1094-1097
Impairment of the circadian clock has been associated with numerous disorders, including metabolic disease. Although small molecules that modulate clock function might offer therapeutic approaches to such diseases, only a few compounds have been identified that selectively target core clock proteins. From an unbiased cell-based circadian phenotypic screen, we identified KL001, a small molecule that specifically interacts with cryptochrome (CRY). KL001 prevented ubiquitin-dependent degradation of CRY, resulting in lengthening of the circadian period. In combination with mathematical modeling, our studies using KL001 revealed that CRY1 and CRY2 share a similar functional role in the period regulation. Furthermore, KL001-mediated CRY stabilization inhibited glucagon-induced gluconeogenesis in primary hepatocytes. KL001 thus provides a tool to study the regulation of CRY-dependent physiology and aid development of clock-based therapeutics of diabetes. 相似文献
69.
Takeda K Tasai M Iwamoto M Oe M Chikuni K Nakamura Y Tagami T Nirasawa K Hanada H Pinkert CA Onishi A 《The Journal of reproduction and development》2012,58(2):248-253
Somatic cell nuclear transfer (SCNT) has been exploited in efforts to clone and propagate valuable animal lineages. However, in many instances, recipient oocytes are obtained from sources independent of donor cell populations. As such, influences of potential nuclear-cytoplasmic incompatibility, post SCNT, are largely unknown. In the present study, alterations in mitochondrial protein levels were investigated in adult SCNT pigs produced by microinjection of Meishan pig fetus fibroblast cells into enucleated matured oocytes (maternal Landrace genetic background). Mitochondrial fractions were prepared from liver samples by mechanical homogenization and differential centrifugation. Liver mitochondria were then subjected to two-dimensional difference gel electrophoresis (2-D DIGE). Protein expression changes were confirmed with a volume ratio greater than 2 fold (P<0.05). 2-D DIGE analysis further revealed differential expression of three proteins between the Meishan (n=3) and Landrace (n=3) breeds. Differential expression patterns of 16 proteins were detected in SCNT pig liver tissue (n=3) when compared with Meishan control samples. However, none of the 16 proteins correlated with the three differentially expressed Meishan and Landrace liver mitochondrial proteins. In summary, alteration of mitochondrial protein expression levels was observed in adult SCNT pigs that did not reflect the breed difference of the recipient oocytes. Comparative proteomic analysis represents an important tool for further studies on SCNT animals. 相似文献