Liquid chromatographic methodology for the characterization of orange juice

Liquid chromatographic (LC) methodology potentially useful for the characterization of orange juice, with particular regard to detecting adulteration of orange juice by computer pattern recognition analysis, has been developed. After dilution with methanol the juice is extracted with hexane to remove the carotenoids, which are chromatographed on a C18 column with an acetonitrile-methanol-methylene chloride mobile phase and detection at 450 nm. Further extraction of the juice with methylene chloride isolates the methoxylated flavones, which are chromatographed by reverse phase LC with an acetonitrile-methanol-water mobile phase and detection at 280 nm. The flavanone glycosides remaining in solution are chromatographed on a C18 column with an acetonitrile-water mobile phase and detection at 280 nm. The precisions of the heights of the 32 LC peaks selected for pattern recognition analysis were determined from 5 replicate analyses of a single juice. Coefficients of variation of the replicates ranged from 0.3 to 4.5%, with an average of 2.1%. Adulteration of products with sodium benzoate-fortified pulpwash or grapefruit juice can be detected by this method. Pattern recognition analysis of the data obtained for 80 authentic and 19 adulterated orange juices showed that the method is potentially useful for distinguishing between authentic and adulterated products.     

Functional expression in pichia pastoris of an acidic pectin methylesterase from jelly fig (Ficus awkeotsang)

A cDNA fragment encoding an acidic pectin methylesterase (PME) of jelly fig achene was successfully expressed in Pichia pastoris under the control of the glyceraldehydes-3-phosphate dehydrogenase promoter. The recombinant PME was produced as a secretory protein by N-terminal fusion of a cleavable prepropeptide for signal trafficking, and thus easily harvested from the culture medium. Compared with native N-glycosylated PME (38 kDa) purified from jelly fig achenes, this recombinant PME (45 kDa) appeared to be hyperglycosylated. Activity staining indicated that the recombinant PME was functionally active. Yet the hyperglycosylated recombinant PME possessed thermostability and enzymatic capability over a broad pH range equivalent to those of the native PME. The success of functional production of this acidic jelly fig PME in P. pastoris has significantly broadened its applications in industry.     

Experienced saxophonists learn to tune their vocal tracts

Acousticians have long debated whether and how the resonances of the vocal tract are involved in the playing of clarinet and saxophone. We measured the resonances of saxophonists' vocal tracts directly while they played. Over most of the instrument's range, there is no simple relation between tract resonances and the note played, and the tract resonances varied among players. In the high (altissimo) range, a strong resonance of the tracts of professional saxophonists was systematically tuned slightly above the desired note. Amateurs, who did not tune a strong resonance, were unable to play notes in the altissimo range.     

Induction of tumors in mice by genomic hypomethylation

Genome-wide DNA hypomethylation occurs in many human cancers, but whether this epigenetic change is a cause or consequence of tumorigenesis has been unclear. To explore this phenomenon, we generated mice carrying a hypomorphic DNA methyltransferase 1 (Dnmt1) allele, which reduces Dnmt1 expression to 10% of wild-type levels and results in substantial genome-wide hypomethylation in all tissues. The mutant mice were runted at birth, and at 4 to 8 months of age they developed aggressive T cell lymphomas that displayed a high frequency of chromosome 15 trisomy. These results indicate that DNA hypomethylation plays a causal role in tumor formation, possibly by promoting chromosomal instability.     

Comparison of QuickBird Satellite Imagery and Airborne Imagery for Mapping Grain Sorghum Yield Patterns

Timely and accurate information on crop conditions obtained during the growing season is of vital importance for crop management. High spatial resolution satellite imagery has the potential for mapping crop growth variability and identifying problem areas within fields. The objectives of this study were to use QuickBird satellite imagery for mapping plant growth and yield patterns within grain sorghum fields as compared with airborne multispectral image data. A QuickBird 2.8-m four-band image covering a cropping area in south Texas, USA was acquired in the 2003 growing season. Airborne three-band imagery with submeter resolution was also collected from two grain sorghum fields within the satellite scene. Yield monitor data collected from the two fields were resampled to match the resolutions of the airborne imagery and the satellite imagery. The airborne imagery was related to yield at original submeter, 2.8 and 8.4 m resolutions and the QuickBird imagery was related to yield at 2.8 and 8.4 m resolutions. The extracted QuickBird images for the two fields were then classified into multiple zones using unsupervised classification and mean yields among the zones were compared. Results showed that grain yield was significantly related to both types of image data and that the QuickBird imagery had similar correlations with grain yield as compared with the airborne imagery at the 2.8 and 8.4 m resolutions. Moreover, the unsupervised classification maps effectively differentiated grain production levels among the zones. These results indicate that high spatial resolution satellite imagery can be a useful data source for determining plant growth and yield patterns for within-field crop management.     

Phonon dispersions of fcc delta-plutonium-gallium by inelastic x-ray scattering

We report an experimental determination of the phonon dispersion curves in a face-centered cubic (fcc) delta-plutonium-0.6 weight % gallium alloy. Several unusual features, including a large elastic anisotropy, a small-shear elastic modulus C', a Kohn-like anomaly in the T1[011] branch, and a pronounced softening of the [111] transverse modes, are found. These features can be related to the phase transitions of plutonium and to strong coupling between the lattice structure and the 5f valence instabilities. Our results also provide a critical test for theoretical treatments of highly correlated 5f electron systems as exemplified by recent dynamical mean field theory calculations for delta-plutonium.