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141.
AIM:To investigate the effect of metallothionein(MT) on proliferation of rat vascular smooth muscle cells (VSMCs) stimulated by homocysteine and its mechanism. METHODS:VSMCs proliferation was measured by [3-H]-TdR incorporation, mitogen-activated protein kinase(MAPK)activity were determined by immunoprecipitation method, the intracellular contents of MT and malondialdehyde (MDA)were assayed by -hemoglobin saturation method and TBA reaction, respectively, and lactate dehydrogenase (LDH) leakage was measured by NADH oxidation. RESULTS:Hcy(10-6-10-4 mmol/L) stimulated [3-H]-TdR incorporation by the VSMCs in a concentration-dependent manner. Compared with control, [3-H]-TdR incorporation in VSMCs treated with 0.1 mmol/L Hcy was increased by 4.2 fold (P<0.01). Meanwhile, Hcy enhanced MAPK activity, MDA formation and LDH release (P<0.01)in a concentration-dependent manner. Treatment of VSMCs with MT alone did not change above parameters, compared with control. However, MT (10-6-10-4 mol/L)attenuated significantly Hcy-stimulated proliferation of VSMCs (P<0.01)in a concentration-dependent manner. And MT inhibited obviously Hcy-induced activation of MAPK activity, MDA formation and LDH release. Preincubation of VSMCs with 0.5 mmol/L ZnCl2 for 6 h induced an increase cellular MT content by 5.7-fold (P<0.01). The MT-overexpressed VSMCs resisted Hcy-stimulating action on MAPK activity, MDA formation and LDH leakage (P<0.01). CONCLUSION:These results show that MT has an inhibitory effect on Hcy-induced VSMCs proliferation, and that MT could inhibit Hcy-stimulated MAPK activity and lipid peroxidation. 相似文献
142.
AIM:To determine the effects of Angiotensin II(AngII) on migration of rat smooth muscle cells and to investigate the mechanisms underlying Ang II action in the development of injured vascular disease. METHODS:VSMCs isolated from aortic media of Wistar rats and cultured by the modified explant method were adopted. In prersence and absence of AngII, the expression of AngII receptor and reorganization of the actin cytoskeleton of VSMCs were studied by immunocytochemistry technique, fluorocytochemistry technique. The migration assays were performed by a modified Boyden's chamber. And the effects of AT1R antagonist (CV-11974), AT2R antagonist (PD123319) on aforementioned target were studied.RESULTS:VSMCs migration was stimulated by addition of AngII. The dynamic reorganization of actin cytoskeleton may be an important mechanism by which AngII facilitates VSMC motility. The expression of AT1R in VSMCs can be upregulated after treatment with AngII initially, then decreased gradually. The expression of AT1R was downregulated by AT1R antagonist. The effect of AngII on VSMCs migration was mediated by AT1R, while AT2R had no significant effect.CONCLUSION:The dynamic reorganization of actin cytoskeleton is required for AngII-induced VSMC migration, and this effect is mediated by AT1R . 相似文献
143.
Protein kinase C pathway is an important intracellular signal transduction pathway. A growing number of evidences showes that activation of PKC influences endothelial cell permeability. In this review, we briefly summarize the effects and regulating pathways of protein kinase C in modulation of vascular endothelium permeability. 相似文献
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5mmol/L水杨酸或1g/L乙烯外源处理可诱导烟草对黑胫病的抗性,推迟植株死亡。不能积累水杨酸的转nahG基因植株和不表现乙烯应答反应的转etr1-1基因植株对黑胫病的抗性显著弱于野生型植株。组成性积累高水平水杨酸的转entC和pmsB基因植株对黑胫病的抗性明显强于野生型植株。这些结果说明水杨酸和乙烯增强烟草对黑胫病的抗性,减缓黑胫病的扩展。0.5mmol/L KCN连续浇灌植株4天或浸溃叶片2,4h均不能恢复转nahG基因植株丧失的对黑胫病的抗病性。此结果进一步支持关于KCN处理不能恢复转nahG基因植株丧失的对真菌病害抗病性的假说。 相似文献
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本文根据在深圳、广州、东莞三地对不同蔬菜、不同农事措施、杀虫剂不同施用水平菜地小菜蛾自然种群幼虫被寄生率调查结果,分析十字花科蔬菜-小菜蛾-菜蛾绒茧蜂三种群系统中,蔬菜品种、农事操作、杀虫剂对菜蛾绒茧蜂寄生行为的影响。结果说明:不同蔬菜品种上,小菜蛾幼虫被寄生率有不同程度的差异,以包心菜菜地寄生率最高,超过30%。十字花科蔬菜与其他非十字花科蔬菜的间作对绒茧蜂的寄生率也存在一定的影响作用。深圳、东莞、广州三个地区,以广州地区小菜蛾幼虫被寄生率较高。化学杀虫剂降低绒茧蜂的寄生率。小菜蛾幼虫的体色与被寄生率也有一定的关系,以体色与背景色一致的个体被寄生率较低 相似文献