周期性测量方法探讨

本文就周期性测量中递推求精方法的可行性进行了一些探讨,并对两个典型实验推导出它们采用递惟求精方法的可行性判别式。     

苏云金杆菌与杀灭菊酯防治红铃虫的试验

苏云金杆菌(Bailus thuringiensis)140或018工业菌剂每亩用200～255克,加杀灭菊酯20%乳剂每亩8毫升混用防治第二、三代红铃虫,虫口减退率和铃害下降率分别为80.5～93.4%和68.8～84.6%;杀灭菊酯每亩40毫升单用效果分别为96～97.2%和84.6～95.8%,都能有效控制红铃虫的危害。棉花产量测定表明,菌剂加菊酯混用使皮棉增加21.3～30.9%,菊酯单用增加11.4%。     

60日龄肉鹅发生小鹅瘟的诊治

吴川市板桥镇某养鹅专业户饲养的一群肉鹅，６０日龄时发生一种以下痢、脱水死亡为特征、伴有阵发性抽搐及扭头神经症状的疫病。经诊断检查，确诊为小鹅瘟病，现将诊治情况报告如下：１发病情况２０００年４月１９日，该专业户共购进雏鹅１８７只，１日龄时注射抗小鹅瘟血清，每只鹅１ｍＬ，育雏期间共死亡３只，尚余１８４只。养至６月１８日时发现病鹅１只，２０日病鹅发展到５只，并出现死鹅１只。至２２日时共发病１０只，死亡５只。发病期间，鹅主曾用土霉素、青霉素、禽菌灵等多种抗菌药物进行治疗，但效果均不理想。２　临床症状　病…     

湖南养猪场污染治理适用技术及投资分析

生猪养殖是湖南传统优势产业,本文总结了湖南生猪养殖污染治理的几种适用技术及投资分析,结果表明:养猪场污染治理应该尽可能地实现资源的最大化利用,使污染治理产生经济收益以调动治污的积极性,从而达到生猪养殖业的可持续发展.     

Comparison of dot blot hybridization, polymerase chain reaction, and virus isolation for detection of bovine herpesvirus-1 (BHV-1) in artificially infected bovine semen.

Bovine semen samples spiked with bovine herpesvirus 1 (BHV-1) were used to compare dot blot hybridization, polymerase chain reaction (PCR), and virus isolation for detection of BHV-1 in bovine semen. The PCR amplification used primers targeting the BHV-1 thymidine kinase gene and a nucleic acid releasing cocktail (GeneReleaser); the PCR product was used as the DNA probe in dot blot hybridization; virus isolation was done in primary bovine fetal testis (BFT) cell cultures. Semen diluted 1:20 in tissue culture medium had the least cytotoxicity and inhibition of viral cytopathic effects in BFT cells, allowing detection of 1 TCID50/100 microL of BHV-1 suspension by virus isolation. The presence of foreign DNA such as bovine sperm DNA or salmon sperm DNA increased the sensitivity of dot blot hybridization in detecting BHV-1, allowing detection of 20,000 TCID50/100 microL of neat semen. The inhibition of PCR amplification of BHV-1 DNA in bovine semen was eliminated by diluting the samples 1:20 in tissue culture medium. The best PCR amplification was obtained when semen was diluted 1:20 and when a reaction buffer of pH 9.0, with 1.0 mM MgCl2 was used. Under these conditions, the PCR followed by ethidium bromide staining of agarose gels could detect 1 TCID20/100 microL of sample, whereas PCR followed by Southern blot hybridization could detect 0.01 TCID50/100 microL of sample.(ABSTRACT TRUNCATED AT 250 WORDS)     

俄罗斯主要采脂树种——欧洲赤松松脂的化学组成

德国Ｗ．Ｌａｎｇｅ在研究不同地区来源的欧洲赤松松脂的化学组成时，曾按△~３－蒈烯含量不同，将它们分成中欧和南欧地区二类。本文研究俄罗斯境内的欧洲赤松松脂的组成，发现它与同纬度的中欧地区欧洲赤松松脂一样，含有较高数量的△~３－蒈烯。同时发现，在中国境内的欧洲赤松的２个变种──樟子松和长白松松脂中△~３－蒈烯含量也随纬度不同而有明显差别。     

Laboratory diagnosis and disease occurrence in the current African swine fever eradication program in Spain, 1989–1991

The purpose of this study was to determine the rate of decline and geographical distribution by municipality of clinical and subclinical African swine fever (ASF) in the affected areas of Spain. A second aim was to evaluate the performance of diagnostic tests in the Spanish ASF eradication program. Clinical outbreaks were confirmed using both the direct and indirect immunofluorescence test (and if both were negative, by the hemabsorption test). The serological status of swine was determined by an enzyme-linked immunosorbent assay (ELISA) and suspect serum samples were confirmed by the immunoblot assay.

The number of clinical outbreaks (herds) of ASF for 1989, 1990 and 1991 was 170, 347 and 207, respectively. The numbers of municipalities within each affected province experiencing acute outbreaks for the same time periods were 49, 69 and 48, respectively. Serologically diagnosed animals positive for ASF were 1.1% of animals tested in 1989, 0.5% in 1990 and 0.8% in 1991. The corresponding positive predictive values of the standard ELISA test used were 99.0, 97.9 and 98.8, respectively. Similarly, the number of municipalities within each affected province experiencing serologically positive subclinically infected animals was 269, 178 and 147 for each of the years 1989, 1990 and 1991, respectively.     

影响老瓜头泌蜜量主导因素的研究

老瓜头自然分布于沙漠地带。在老瓜头开花期,天气变化无常。我们研究了对老瓜头泌蜜量起关键影响作用的气象因子。根据当地气候的变化规律进行预测预报,指导养蜂者正确选择场地,以夺取老瓜头蜜高产。