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11.
Isolation of influenza A viruses from migratory waterfowl in San-in District, western Japan in the winter of 1983-1984 总被引:1,自引:0,他引:1
K Otsuki O Takemoto R Fujimoto K Yamazaki N Kubota H Hosaki T Mitani M Tsubokura 《Research in veterinary science》1987,43(2):177-179
Certain species of winter migratory waterfowl in San-in District, western Japan, were surveyed for influenza virus from November 1983 to March 1984. Faeces of the waterfowl were collected regularly at five stations. Eleven influenza A viruses including H5N3 and H10N4 subtypes were isolated from 450 faecal samples from whistling swans, 28 viruses including H2N2 and H10N4 subtypes were isolated from 362 faecal samples from pintails; and subtype H13N6 was isolated from 240 faecal samples of black-tailed gulls. 相似文献
12.
Detection of the esp gene in high-level gentamicin resistant Enterococcus faecalis strains from pet animals in Japan 总被引:1,自引:0,他引:1
We investigated the prevalence of the esp gene and the susceptibility to gentamicin in Enterococcus faecalis and E. faecium strains obtained from pet animals. Nine of 30 E. faecalis and 2 of 38 E. faecium strains from the pet animals had the esp gene. Three esp-positive E. faecalis strains, which were isolated from two dogs and a cat, showed gentamicin MICs of > or =256 microg/ml and harbored the high-level gentamicin resistance (HLGR) gene, aac(6')-Ie-aph(2')-Ia. Of the nine esp-positive E. faecalis strains, five, including the three strains with the HLGR gene, were closely related by numerical analysis of PFGE patterns. Longitudinal investigation needs to elucidate whether the HLGR gene was incorporated into a subpopulation of the esp-positive E. faecalis. 相似文献
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Denise T. Fantoni Keila K. Ida André M. Gimenes Matheus M. Mantovani Jacqueline R. Castro Geni C.F. Patrício Aline M. Ambrósio Denise A. Otsuki 《Veterinary anaesthesia and analgesia》2017,44(4):710-718
Objective
To investigate whether pulse pressure variation (PPV) can predict fluid responsiveness in healthy dogs during clinical surgery.Study design
Prospective clinical study.Animals
Thirty-three isoflurane-anesthetized dogs with arterial hypotension during orthopedic surgery.Methods
Fluid challenge with lactated Ringer's solution (15 mL kg?1 in 15 minutes) was administered in mechanically ventilated dogs (tidal volume 10 mL kg?1) with hypotension [mean arterial pressure (MAP) < 65 mmHg]. The volume expansion was considered effective if cardiac output (CO; transesophageal Doppler) increased by ≥ 15%. Cardiopulmonary data were analyzed using two-way ANOVA, receiver operating characteristics (ROC) curves and Spearman coefficient; p < 0.05 was considered significant.Results
Effective volume expansion, mean ± standard deviation 42 ± 4% increase in CO (p < 0.0001) was observed in 76% of the dogs, resulting in a decrease in PPV (p < 0.0001) and increase in MAP (p < 0.0001), central venous pressure (CVP; p = 0.02) and ejection fraction (p < 0.0001) compared with before the fluid challenge. None of these changes occurred when volume expansion resulted in a nonsignificant CO increase of 4 ± 5%. No significant differences were observed in blood gas analysis between responsive and nonresponsive dogs. The increase in CO was correlated with the decrease in PPV (r = ?0.65; p < 0.0001) but absolute values of CO and PPV were not correlated. The PPV performance (ROC curve area: 0.89 ± 0.06, p = 0.0011) was better than that of CVP (ROC curve area: 0.54 ± 0.12) and MAP (ROC curve area: 0.59 ± 0.13) to predict fluid responsiveness. The best cut-off for PPV to distinguish responders and nonresponders was 15% (50% sensitivity and 96% specificity).Conclusions and clinical relevance
In mechanically ventilated, healthy, isoflurane-anesthetized dogs, PPV predicted fluid responsiveness to volume expansion, and MAP and CVP did not show such applicability. 相似文献16.
Kenji Tsuruta Tomonori Kume Hikaru Komatsu Naoko Higashi Toshihiro Umebayashi Tomo’omi Kumagai Kyoichi Otsuki 《Journal of Forest Research》2010,15(6):398-403
Sap flow techniques are practical tools for estimating tree transpiration. Though many previous studies using sap flow techniques
did not consider azimuthal variations of sap flux density (F
d) on xylem trunk to estimate tree transpiration, a few studies reported that ignoring the azimuthal variations in F
d could cause large errors in tree transpiration estimates for some tree species. Therefore, examining azimuthal variations
in F
d for major plantation tree species is critical for estimating tree transpiration. Using the thermal dissipation method, we
examined azimuthal variations in F
d in six trees of Japanese cypress Chamaecyparis obtusa (Sieb. et Zucc.) Endl., which is one of the most common plantation tree species in Japan. We recorded considerable variations
among F
d at four different azimuthal directions. The F
d value for one aspect was more than 100% larger than those for the other aspects. We calculated differences between tree transpiration
estimates based on F
d for one to three azimuthal directions and those based on F
d for four aspects. The differences relative to tree transpiration estimates based on F
d for four aspects were typically 30, 20, and 10% in accordance with the F
d for one, two, and three measurement aspects, respectively. This finding indicates that ignoring azimuthal variations could
cause large errors in tree transpiration estimates for Japanese cypress. 相似文献
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18.
Kyoichi Kumada 《Soil Science and Plant Nutrition》2013,59(1):29-30
For the purpose of explaining the forming process of soil humic acids, the author determined the absorption spectra of various humic acids. From soils and peats which were pretreated with 5% HCl at 70°C for 30 minutes or from those which were not, humic acids were extracted by treating with 0.5% NaOH at boiling temperature for 30 minutes. In these humic acids, the one which is extracted after acid pretreatment is provisionally designated as SrL humic acid and the other as L humic acid. The supernatant alkaline solutions obtained by centrifuging the above mentioned extracts were acidified with hydrochloric acid, and precipitated humic acids were filtered and washed with water until Cl' free. Humic acids were dissolved in 0.1% NaOH and ultrafiltered using collodion membrane. The filtrates were acidified with hydrochloric acid and humic acids were collected by centrifuging, transferred on the filter paper, washed with dilute hydrochloric acid and water successively, then air-dried and pulverized. 相似文献
19.
Kyoichi Kumada 《Soil Science and Plant Nutrition》2013,59(1):106-111
I. Absorption Spectra of Some Artificial Humic Acids It is a well known fact that numerous dark brown or black and amorphous substances cap be produced from organic compounds by purely chemical processes. Some of them are called artificial humic acids, and have been used as a model of soil humic acids by many workers. But it is doubtful whether such artificial humic acids are considered to be a model of soil humic acids, even if there can be found any similarities in their properties. However it may be an useful method for the study of humus formation to compare artificial humic acids with soil humic acids, and to make efforts to find a good model or to prepare artificial humic acids comparable to soil humic acids under laboratory conditions. For this purpose, first of all, the author prepared artificial humic acids from glucose, hydroquinone and lignin, and their absorption spectra were determined. 相似文献
20.
P type humic acid has absorption bands near 615, 570 and 450mµ in the visible region, and is a common component of many soils in north temperate regions (1, 2, 3). Its unusual spectral characteristics are due to a green fraction (Pg), which can be separated from the humic acid dissolved in aqueous alkali by chromatography on Sephadex or cellulose powder, and is precipitated as a reddish-brown substance on acidification (2). In aqueous alkali, it has λmax at 613, 568, 448, 430 (infl.) and 281mµ. The distribution of Pg in different soils is probably related to the presence of fungal sclerotia. The highest content of Pg in p type humic acid is about 10%. We have now obtained evidence that the characteristic colour of Pg is due to the presence of a derivative of 4,9 - dihydroxyperylene - 3,10 - quinone. 相似文献