Paleoclimatic evidence for future ice-sheet instability and rapid sea-level rise

Sea-level rise from melting of polar ice sheets is one of the largest potential threats of future climate change. Polar warming by the year 2100 may reach levels similar to those of 130,000 to 127,000 years ago that were associated with sea levels several meters above modern levels; both the Greenland Ice Sheet and portions of the Antarctic Ice Sheet may be vulnerable. The record of past ice-sheet melting indicates that the rate of future melting and related sea-level rise could be faster than widely thought.     

Synthesis and characterization of the nitrides of platinum and iridium

Transition metal nitrides are of great technological and fundamental importance because of their strength and durability and because of their useful optical, electronic, and magnetic properties. We have evaluated a recently synthesized platinum nitride (PtN) that was shown to have a large bulk modulus, and we propose a structure that is isostructural with pyrite and has the stoichiometry PtN2. We have also synthesized a recoverable nitride of iridium under nearly the same conditions of pressure and temperature as PtN2. Although it has the same stoichiometry, it exhibits much lower structural symmetry. Preliminary results suggest that the bulk modulus of this material is also very large.     

Selective stimulation of T cell subsets with antibody-cytokine immune complexes

Interleukin-2 (IL-2), which is a growth factor for T lymphocytes, can also sometimes be inhibitory. Thus, the proliferation of CD8+ T cells in vivo is increased after the injection of a monoclonal antibody that is specific for IL-2 (IL-2 mAb), perhaps reflecting the removal of IL-2-dependent CD4+ T regulatory cells (T regs). Instead, we show here that IL-2 mAb augments the proliferation of CD8+ cells in mice simply by increasing the biological activity of preexisting IL-2 through the formation of immune complexes. When coupled with recombinant IL-2, some IL-2/IL-2 mAb complexes cause massive (>100-fold) expansion of CD8+ cells in vivo, whereas others selectively stimulate CD4+ T regs. Thus, different cytokine-antibody complexes can be used to selectively boost or inhibit the immune response.     

Femtosecond multidimensional imaging of a molecular dissociation

The coupled electronic and vibrational motions governing chemical processes are best viewed from the molecule's point of view-the molecular frame. Measurements made in the laboratory frame often conceal information because of the random orientations the molecule can take. We used a combination of time-resolved photoelectron spectroscopy, multidimensional coincidence imaging spectroscopy, and ab initio computation to trace a complete reactant-to-product pathway-the photodissociation of the nitric oxide dimer-from the molecule's point of view, on the femtosecond time scale. This method revealed an elusive photochemical process involving intermediate electronic configurations.     

Integrating local breeding pond, landcover, and climate factors in predicting amphibian distributions

Species distributions are influenced by many processes operating over varying spatial scales. The development of species distribution models (SDMs), also known as ecological niche models, has afforded the opportunity to predict the distributions of diverse taxa across broad geographic areas and identify variables that are potentially important in regulating these distributions. However, the integration of site-specific habitat data with broad scale climate and landcover data has received limited attention in an SDM framework. We investigate whether SDMs developed with breeding pond, landcover, and climate variables can accurately predict the distributions of nine pond-breeding amphibians in eastern Missouri, USA. Additionally we investigate the relative influences of each environmental variable on the distribution predictions for each study species, and whether the most influential variables are shared among multiple taxa. Boosted regression tree (BRT) SDMs were developed for each species with 38 abiotic and biotic environmental variables, including data from the breeding ponds, surrounding landcover, and climate. To test the models, field surveys were performed in 2007 and 2008 at 103 ponds for nine amphibian species. BRT models developed with breeding pond, landcover, and climate data accurately predicted the occurrences of six of nine species across the study area. Furthermore, the presence of each species was best predicted by a unique combination of environmental variables. Results also suggest that landcover and climate factors may be more influential for species near the edge of their geographic ranges, while local breeding pond factors may be more important for species nearer to the center of their ranges.     

Ultrasonographic measurement of flow-mediated vasodilation in dogs with chronic valvular disease

ObjectivesTo measure flow-mediated vasodilation (FMD) in healthy dogs and in client-owned dogs with chronic valvular disease (CVD) and to investigate possible correlations between markers of CVD severity and FMD.AnimalsTwelve dogs with CVD and 11 healthy weight-matched dogs.MethodsBrachial artery FMD following 5 min inflation of a cuff around the antebrachium was measured in 12 dogs with CVD and 11 healthy weight-matched dogs. Measurements were also obtained in the healthy dogs 5 min after cuff placement but without inflation (‘sham cuff placement’). Dogs with CVD underwent echocardiography to confirm and characterize their disease.ResultsIn healthy dogs (median age 4 [2–6] years), median FMD was 7.7% versus 3.4% with sham cuff placement (P = 0.003). In dogs with CVD (median age 8 [4–16] years) median FMD was 5.5% versus 7.7% in healthy dogs (P = 0.131). FMD showed an inverse correlation with left ventricular end-diastolic diameter normalized for body weight (r = ?0.76, P = 0.0043).ConclusionsBrachial FMD in dogs with early CVD inversely correlates with severity of left ventricular remodelling.     

Within-day and between-day variability of transthoracic anatomic M-mode echocardiography in the awake bottlenose dolphin (Tursiops truncatus)

The use of transthoracic echocardiography in dolphins has been limited so far owing to technical and anatomical specificities. Anatomic M-mode (AMM) is a postprocessing echocardiographic technique generating M-mode studies from two-dimensional (2D) cineloops independently of the ultrasound beam orientation. The aim of the present study was to determine the within-day (repeatability) and between-day (reproducibility) variability of AMM echocardiography in awake healthy bottlenose dolphins (BN, Tursiops truncatus). Four adult BN trained to lie in left recumbency at the water surface were involved in the protocol. A total of 96 echocardiographic examinations were performed on 4 different days by a trained observer examining each BN 6 times per day. Video clips of 2D left parasternal long-axis views showing the left ventricle (LV) ventrally and the aortic root dorsally were recorded at each examination and analyzed for AMM measurements in a random order. A general linear model was used to determine the within-day and between-day coefficients of variation (CV). All examinations were interpretable allowing calculation of 10 AMM variables (i.e., end-diastolic and end-systolic ventral and dorsal LV myocardial wall thicknesses as well as LV and aortic diameters, mean aortic diameter, and LV shortening fraction). Most within- and between-day CV values (18/20) were <15%, the lowest being observed for the end-diastolic LV diameter (1.6%). In conclusion, AMM provides a simple non-invasive evaluation of heart morphology and function in the awake BN with good repeatability and reproducibility of the measurements. Further studies are required to determine the corresponding reference intervals.     

Soil microbial biomass and population in response to seasonal variation and age in Gmelina arborea plantations in south-western Nigeria

We investigated the Effects of plantation development, seasons, and soil depth on soil microbial indices in Gmelina arborea plantations in south-western Nigeria. Soil samples were obtained from the soil depths of 0-15 and 15-30 cm from plantations of six different ages during the rainy season, dry seasons, and their transitions. We used plate count and fumigation-extraction methods to determine microbe population and microbial biomass carbon (MB-C) and nitrogen (MB-N), respectively. Plantation age did not affect microbial indices, implying a non-significant effect of plantation development on microbial communities. It could also imply that soil microbial indices had already stabilized in the sampled plantations. Seasonal variation and soil depth had significant effects on microbial indices. At 0-15 cm soil depth, mean MB-C increased from 50.74 μg g-1 during the peak of the dry season (i.e. March) to 99.58 μg g-1 during the peak of the rainy season (i.e. September), while it increased from 36.22 μg g-1 to 75.31 μg g-1 at 15-30 cm soil depth between the same seasonal periods. Bacteria populations and MB-N showed similar increasing trends. Correlations between MB-C, MB-N, microbe populations, and rainfall were positive and linear. Significantly higher microbial activities took place in the plantations during the rainy season, increased with soil wetness, and decreased at greater soil depth.     

A novel technique for non-damaging measurement of sound speed in seedlings

A cross-correlation time-of-flight method for measuring the sound speed in a seedling in a non-destructive and non-damaging way is described. The method uses two miniature accelerometers coupled to the bark of a growing seedling stem to record the acoustic signals and a small striker to generate an acoustic pulse. The two acoustic signals are then time-windowed and cross-correlated in order to calculate the speed of the acoustic pulse. The method measures the sound speed of the seedling stem as a whole and does not selectively measure the fastest path. The method was tested on 1- and 2-year-old Pinus radiata clones. A comparison of the results of the method with a destructive acoustic resonance test showed that the sound speed results are unbiased. Repeatability tests give a standard deviation of the sound speed of less than 2%. The method shows good promise as a rapid and cost-effective tool for early screening of wood quality in clonal trials.