Population parameters for birth and ewe fleece weight at different parities in Baluchi sheep

ZUSAMMENFASSUNG: Populationsparameter für Geburts- und Vlie?gewicht von Baluchi Schafen Das Datenmarterial stammt von zwei Herden einer Schafzuchtstation in NO Iran aus den Jahren 1966-1989. Die Tiere waren unselektiert und stammten aus zuf?llig verteilten Paarungen. Es wurden Geburtsgewicht und Vlie?gewicht bei verschiedenen Altersstufen erhoben und Varianzkomponenten mittels Restringierter Maximaler Likelihood mit einem bivariaten Tiermodell mit fixen Wirkungen von Jahr, Geschlecht, Geburtstyp und Parit?t sowie Zufallswirkungen für additiven Genotyp des Lammes (direkt) und des Mutterschafes (maternal), gemeinsamer Umwelt (ausgenommen Vlie?gewicht) und Resteinflu? gesch?tzt. Direkte und maternale genetische Korrelationen zwischen Leistungen verschiedener Parit?ten wurden berechnet. In Herde 1 scheinen Varianzen und Heritabilit?tswerte für Lammgewicht bis Parit?t 5 zuzunehmen, kaum aber in Herde 2. Die durchschnittlichen Heritabilit?tswerte, direkt, maternal und gesamt waren 0.12, 0.11 und 0.26, die genetische Korrelation zwischen direkten und maternalen Wirkungen 0.42. Bei Vlie?gewicht waren in Herde 1 keine Ver?nderungen der Varianzen und Heritabilit?tswerte mit Alter zu erkennen, aber bei Herde 2 nahmen ph?notypische und Umweltvarianz mit Alter leicht zu. Durchschnittliche direkte, maternale und Gesamtheritabilit?t waren 0.19, 0.04 und 0.22, die genetische Korrelation zwischen direkten und maternalen Wirkungen geringgradig positiv in Herde 1, aber mit Alter zunehmend negativ in Herde 2. Die genetischen Korrelationen für direkte Wirkungen auf Geburtsgewicht waren hoch zwischen Parit?ten 1 bis 5, aber niedriger bei Parit?t 6 und jene zwischen maternal bedingten Wirkungen zeigten ?hnliche Trends. In Herde 2 waren Werte mit Parit?t 6 ?hnlich wie die zwischen den übrigen Parit?ten. Die die Vlie?gewichte betreffenden direkt genetischen Korrelationen zwischen Parit?ten waren in beiden Herden ?hnlich (0.73-0.92), jene, die maternale Wirkungen betreffen, deutlich geringer, besonders soweit sie Parit?ten 5 und 6 betroffen haben und zeigten besonders bei Herde 2 starke Schwankungen (-0.54 bis 0.74). SUMMARY: Direct and maternal performance of ewes at different parities were examined in Baluchi sheep. The data set was collected during the period 1966-1989 from two flocks at a sheep breeding station in the north-east of Iran. The animals included in the data set were unselected and randomly mated. The traits analysed were birth weight of lamb and fleece weight at different parities of the ewe. Variance components were estimated using Restricted Maximum Likelihood with a bivariate animal model including fixed effects of year, sex, type of birth and parity, and random effects of additive genotype of lamb (direct genetic effect), additive genotype of ewe (maternal genetic effect) and common environment (excluded for ewe fleece weight), and random residual effect. Direct and maternal genetic correlations between different parities were estimated. There was evidence of increasing phenotypic and genetic variances and heritabilities from parity 5 for birth weight of lamb in flock 1, but only evidence of a slightly increasing age trend for the environmental and phenotypic variance in flock 2. The average heritabilities over flocks and parities were 0.12, 0.11 and 0.26 for the direct, maternal and total heritability, respectively, while the average genetic correlation between direct and maternal effects for this trait was 0.42. There were no indications of any age changes in variances or heritabilities for ewe fleece weight in flock 1, but indications of slightly increasing age trends for the environmental and phenotypic variance. The average heritabilities over flocks and parities were 0.19, 0.04 and 0.22 for the direct, maternal and total heritability, respectively, while the average genetic correlation between direct and maternal effects was slightly positive in flock 1 but increasingly negative with age of the ewe in flock 2. Direct genetic correlations between parities 1-5 were very high for birth weight of lambs (on average 0.96) in contrast to the markedly lower correlations of parities 1-5 with parity 6 (on average 0.67) in flock 1 with a similar pattern for the maternal genetic correlations. In flock 2, these correlations were also high but without the marked decrease between parities 1-5 with parity 6 that was found in flock 1. Direct genetic correlations between the various parities for ewe fleece weight were similar for the two flocks, ranging from 0.73 to 0.92 and without any obvious differences between the various combinations of parities. However, the maternal were markedly lower than the direct genetic correlations, especially for the combinations of parity 5 and 6 with the earlier parities, and most pronounced in flock 2 fluctuating from -0.54 to 0.79. To obtain reliable estimates of breeding values for birth weight of lamb, it is recommended that the prediction should include not only earlier but also later parities (ages) of the ewe.     

Detection and Identification of Mycoplasma conjunctivae in Infectious Keratoconjunctivitis by PCR. Based on the16S. rRNA. Gene

A specific PCR assay based on unique sequences of the rrs genes (16S rRNA) of Mycoplasma conjunctivae was developed for direct detection and identification of this pathogen from clinical material. DNA from eye swabs was amplified after a simple lysis step by either a single PCR with the M. conjunctivae specific primer pair McoR1 and McoF1, or by a nested PCR with the Mycoplasma genus specific primer pair MOLIGEN1-L and 16UNI-R in the first step and McoR1 and McoF1 in the second step. The specificity of the primer pair McoR1 and McoF1 was verified with purified DNA from the type strain, from 17 field isolates of M. conjunctivae and from several Mollicutes which are phylogenetically related to M. conjunctivae or which can be isolated from the same host animals. This method identified mycoplasma isolates from goat, sheep, ibex and chamois originating from different countries as M. conjunctivae. No cross amplifications with other mycoplasmas which are related to M. conjunctivae were observed. Eye swab samples containing known numbers of M. conjunctivae cells were analysed after direct lysis of the material. The detection level was estimated to be 20 cells per swab when the nested PCR procedure was used and 2 × 10⁵ by the single PCR method. In an experimental infection model of sheep, the nested PCR method for detection of M. conjunctivae gave results which were comparable to mycoplasmal culture. These are the implications for diagnostic purposes: M. conjunctivae isolates can be identified by the one-step PCR method, whereas for detection and identification of M. conjunctivae in clinical material the two-step method should be used (higher sensitivity).     

Mechanical fiber separation under torsional stress

Summary Spruce wood (Picea abies) samples were pretreated at different temperatures and, in some cases, with sodium sulfite solution under normal conditions for the chemimechanical pulping of wood chips. The pretreated samples were simultaneously subjected to torsion and compression stresses at temperatures ranging from 20 to 90 °C. The load-deformation relationship was analyzed at each temperature. The failure zones were studied using the scanning electron microscope technique. The results showed that the maximum torque decreased with increasing temperature under the deformation conditions applied. Sulfonation of the samples gave a similar effect, although to a smaller extent. The elastoplasticity of the samples, as viewed in terms of the twist angle at failure, was also affected by the pretreatment. While axial compression had a major effect, sulfonation only caused a marginal change. Microscopic studies of the failure zone showed that when the temperature increased, the fracture plane traveled around fibers instead of through them, thus causing less fiber damage. Sulfonation improved fiber separation and decreased fiber damage. The separation of ray cells from the fiber tracheids was improved, particularly by sulfonation. Increasing temperature and sulfonation changed the fracture plane from the secondary wall of fibers towards the more lignin enriched primary wall and middle lamella. Received 29 January 1997     

Detection and identification of Mycoplasma conjunctivae in infectious keratoconjunctivitis by PCR based on the 16S rRNA gene.

A specific PCR assay based on unique sequences of the rrs genes (16S rRNA) of Mycoplasma conjunctivae was developed for direct detection and identification of this pathogen from clinical material. DNA from eye swabs was amplified after a simple lysis step by either a single PCR with the M. conjunctivae specific primer pair McoR1 and McoF1, or by a nested PCR with the Mycoplasma genus specific primer pair MOLIGEN1-L and 16UNI-R in the first step and McoR1 and McoF1 in the second step. The specificity of the primer pair McoR1 and McoF1 was verified with purified DNA from the type strain, from 17 field isolates of M. conjunctivae and from several Mollicutes which are phylogenetically related to M. conjunctivae or which can be isolated from the same host animals. This method identified mycoplasma isolates from goat, sheep, ibex and chamois originating from different countries as M. conjunctivae. No cross amplifications with other mycoplasmas which are related to M. conjunctivae were observed. Eye swab samples containing known numbers of M. conjunctivae cells were analysed after direct lysis of the material. The detection level was estimated to be 20 cells per swab when the nested PCR procedure was used and 2 x 10(5) by the single PCR method. In an experimental infection model of sheep, the nested PCR method for detection of M. conjunctivae gave results which were comparable to mycoplasmal culture. These are the implications for diagnostic purposes: M. conjunctivae isolates can be identified by the one-step PCR method, whereas for detection and identification of M. conjunctivae in clinical material the two-step method should be used (higher sensitivity).     

Single-step divergent selection for male comb shape in two White Leghorn lines.

1. Divergent selection for comb shape (SH, the way that the cockerel bears the comb) was performed in 2 White Leghorn lines in a study aimed at assessing possibilities for improving SH. 2. Line A, selected for large comb size (CS) at 29 weeks of age, had great SH problems whereas Line H, selected for high hyaluronic acid concentration in the comb (HA), had minor SH problems. Multivariate analyses were used to estimate genetic parameters for SH, CS and HA in lines A and H and in a control line (C). 3. Significant direct selection responses for SH were achieved in both lines. There were significant unfavourable correlated responses for CS in both lines. 4. The correlated responses for HA were not significant and were unfavourable in Line A and favourable in Line H. 5. Heritabilities for SH and CS were high in both lines and relatively low for HA. Most of the genetic correlations were in agreement with the correlated responses obtained.     

Surfactant Protein C in Canine Pulmonary Fibrosis

Background: Canine pulmonary fibrosis (CPF) occurs most commonly in West Highland White Terriers. The differing incidences of CPF among dog breeds suggest that genetic factors contribute to its pathophysiology. Pulmonary fibrosis in humans is associated with mutations in the gene coding for lung surfactant protein C (SP-C) ( SFTPC ).
Hypothesis/Objectives: To investigate the histopathologic changes and SP-C composition and genetic structure in dogs with CPF.
Animals: Five dogs with PF, 2 dogs with other lung diseases, and 3 healthy dogs.
Methods: Lung tissue from dogs with clinically suspected CPF and 5 control cases was analyzed histopathologically. Bronchoalveolar lavage fluid (BALF) collected postmortem from 3 terriers with histopathologically confirmed pulmonary fibrosis and the 5 controls were analyzed by Western blots, and the exons of SFTPC were sequenced for 2 dogs with PF and 1 dog with other lung disease.
Results: SP-C could not be detected in BALF of 1 dog with PF, although SP-B was present. A mutation was detected in SFTPC exon 5 of this dog. From 2 dogs with PF and in all 5 control dogs SP-B and SP-C were detected in BALF.
Conclusions: Taken together, the results indicate that canine and human lung fibrosis share histopathologic features and that analysis of SP-C and its gene in a larger set of dogs with PF is warranted.     

Finite element study of growth stress formation in wood and related distortion of sawn timber

Lack of straightness in timber is the most frequent complaint regarding solid (and laminated) timber products worldwide. Nowadays, customers demand higher quality in the shape stability of wood products than they did earlier. The final distortion of timber boards is mostly caused by moisture-related stresses in wood (drying distortions) and growth-related stresses (distortions appearing when logs are split up to timber boards by sawing). To get more knowledge on how these distortions can be reduced in wooden products, there is a need for improved understanding of this material behaviour through good numerical tools developed from empirical data. A three-dimensional finite element board distortion model developed by Ormarsson (Doctoral thesis, Publ. 99:7, 1999) has been extended to include the influence of growth stresses by incorporating a one-dimensional finite element growth stress model developed here. The growth stress model is formulated as an axisymmetric general plane strain model where material for all new annual rings is progressively added to the tree during the analysis. The simulation results presented include how stresses are progressively generated during the tree growth, distortions related to the redistribution of growth stresses during log sawing, and distortions and stresses in drying reflecting the effects of growth stresses. The results show that growth stresses clearly vary during tree growth and also form a large stress gradient from pith to bark. This in itself can result in significant bow and crook deformations when logs are sawn into timber boards. The distortion results from the simulations match well with the results observed in reality. The parametric study also showed that the radial growth stress distribution is highly influenced by parameters such as modulus of elasticity, micro fibril angle and maturation strain.     

Genetic trends for fertility,udder health and protein yield in Swedish red cattle estimated with different models

The aim of this study was to estimate and compare genetic trends in Swedish Red cattle using a full multiple-trait (MT) model and trait-group-wise models for female fertility, udder health and protein yield. Field data for maiden heifers from 1989 and cows with a first and second lactation between 1990 and 2007 were included. (Co)variance components were estimated prior to prediction of breeding values. The estimated genetic trends were clearly favourable for protein yield and udder conformation, and in most cases neutral to favourable for clinical mastitis and calving to first insemination. In maiden heifers, the trends were neutral for number of inseminations per service period. Unfavourable genetic trends were estimated for number of inseminations in the first two lactations, but the trends seemed less unfavourable from evaluations within trait groups compared with when using the full MT model. Excluding maiden heifer data affected genetic trends less than using trait-group-wise analyses instead of a full MT model. Unfavourable genetic trends in functional traits may be missed unless the traits are evaluated in a MT model including traits under strong selection.     

Detection of Chalara fraxinea from tissue of Fraxinus excelsior using species‐specific ITS primers

Chalara fraxinea (teleomorph: Hymenoscyphus albidus) is known as a serious pathogen of Fraxinus excelsior, causing massive dieback of trees in Europe. The fungus is able to cause latent infections, and has been previously detected as an endophyte in asymptomatic tissues. Chalara fraxinea is a slow grower in culture, and is thus likely to be overgrown by faster growing fungi whenever pure culture isolations are being attempted. This study reports species‐specific ITS primers allowing fast and reliable detection of the pathogen directly from infected tissues of F. excelsior.     

Concentrations and fluxes of dissolved organic carbon and nitrogen in a Picea abies chronosequence on former arable land in Sweden

Shifting land use from agriculture to forestry induces major changes in the carbon (C) and nitrogen (N) cycles, including fluxes of dissolved organic carbon (DOC) and nitrogen (DON). This study investigated the long-term effects of afforestation on ecosystem DOC and DON dynamics using a chronosequence approach comprising four arable fields and nine differently aged (10–92 years) Norway spruce stands growing on similar former arable soils in the same area. Along the chronosequence, concentrations and fluxes of DOC and DON were determined in bulk precipitation, throughfall, O horizon leachate and mineral soil solution during a 2–3-year period. Soil water fluxes were calculated using a soil hydrological model (SWAP). Results showed that DOC concentrations and fluxes with throughfall were strongly positively correlated with tree height (r² = 0.95; P < 0.05 for both conc. and flux) and stand age, while DON showed no such trends, suggesting different origins of DOC and DON in throughfall. The highest concentrations and fluxes of DOC and DON occurred in soil leachate from the O horizon. Here, DOC flux was 250–310 kg C ha⁻¹ yr⁻¹ and DON flux 8–9 kg N ha⁻¹ yr⁻¹ in stands afforested between 65 and 92 years ago. Concentrations and fluxes of DOC and DON in the mineral subsoil were consistently low. Flux calculations suggest that there was a net loss of >90% (230–280 kg ha⁻¹ yr⁻¹) of DOC leached from the O horizon within 0–60 cm of the mineral soil. There was no significant effect of land use or forest age on DOC concentrations in solution from the lower part of the A horizon. The effect of time since afforestation was masked by soil properties that influence DOM retention in the mineral soil. Our data indicate that DOC concentrations in the A horizon of the sites studied were primarily related to the oxalate-extractable Al and Fe amounts in the same horizon. Afforestation of arable land induced a gradual qualitative change in soil organic matter (SOM) and dissolved organic matter (DOM), with significantly increasing C:N ratios in soil and soil solution over time. The development of an O horizon and the subsequent leaching of DOC and DON to the underlying mineral soil are important drivers of a changing soil C and N turnover following afforestation.