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81.
播娘蒿油酸脱氢酶基因(DsFAD6)的克隆和表达分析 总被引:1,自引:0,他引:1
采用RACE-PCR的技术克隆了播娘蒿的油酸脱氢酶基因(DsFAD6),它是催化油酸转化为亚油酸的一个关键酶基因.DsFAD6 cDNA全长序列的开放阅读框长1 344 bp,编码一个由447个氨基酸残基组成的蛋白质,推测的蛋白质相对分子质量为51.16 kD,等电点为9.27.序列分析表明DsFAD6的氨基酸序列具有3个高度保守的组氨酸盒子,该结构在去饱和酶中是高度保守的,且在N端预测到信号肽,初步认定该酶定位于质体.序列比对和系统发生分析显示DsFAD6与十字花科植物FAD6基因具有较高一致性.RT-PCR分析表明,DsFAD6在根、茎、叶、花蕾、花及幼嫩角果中均表达,但在茎、叶和幼嫩角果中表达较高.胁迫诱导表达中,DsFAD6在播娘蒿叶片中表达明显受伤害胁迫诱导,但在冷胁迫下呈负相关. 相似文献
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This article presents an implement of component using COM technology for collecting environmental monitoring data in environment monitoring system. The component makes the separation of data collection and data analysis available.Simultaneously by using the interface of connectable object in COM,itmakes the cooperation of the environment monitoring software with other parts of fault management software available,and also the seamless connection.It improves the system's performance by adding extensibility and potential cooperatibility. 相似文献
85.
In order to research the state of energy bands and gain and loss of electrons on the surface of ZnSnO_(3),it is put forward that Pinning theory of Fermi energy is applied to development of gas sensitive materials.The relative position between surface state and conduction band on surface of zinc tin complex oxide(ZnSnO_(3)) gas sensitive material has been investigated by utilizing oxidation redox couple,FeCl_(2)/ FeCl_(3),which have high surface state.Fermi energy is pinned at about 0.2 eV below conduction band E_(cs)on the surface of ZnSnO_(3).It has been determined that additives doping ratio,addition concentration and working atmosphere have no effect on this value.Therefore,pinning theory of Fermi energy has been verified by using this method. 相似文献
86.
Though the Flood-Control-Projects along Yantze River and Jialing River in Chongqing may well improve the urban traffic and construction,it may change the river hydrodynamic characters.There may be two consequences,one is to reduce the size of cross section and the other is to change the river shape,such as the curvature radius.The phenomena of the local backwater and velocity variety are obvious,which result in some problems for the navigation: sediment,transport and so on.In this paper, the study object is the Flood-Control-Projects and Yangtse River and Jialing River,and the aim is to study the influence of the Flood-Control-Projects on the hydrodynamic state of the two rivers.Using the numerical technique,with the two rivers hydrodynamic model the flow field's change of river after completion of Flood-Control-Projects is simulated and the reasons caused by the change are analyzed. 相似文献
87.
The tensile strength of high strength bolt is affected by many factors, including prying force. The prying force can result in the increment of bolt force, for which the connection failed ahead of time. Several methods are presented for calculating the prying force. In the China's "Code for Design of Steel Structure" (GB50017), there is no formula for calculation of prying force and a factor of 0.8 is considered in formula for tensile strength of bolt for taking the prying force into account. It is proposed that the design value of tensile resistance for high strength bolt should be increased. 相似文献
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为了选育持久抗耐多种病害的小麦核心抗病种质,以奥地利黑麦、中间偃麦草和野生二粒小麦为抗源,采用单交、复合杂交、回交、阶梯式杂交等多种组配方式,将基因逐步累加、聚合到一起。选育中以不丢失抗性基因为前提,以抗旱耐寒为适应性选择的基本标准。育成的小麦新品系远丰139半冬性,耐寒、抗旱,株型结构好,产量高,品质好。经西北农林科技大学植保系鉴定对条锈病免疫,对白粉病和叶枯病高抗,中抗赤霉病。经农业部谷物品质检测中心分析,品质达到国标精制级面条用小麦粉标准。2005年陕西省区试较对照小偃22增产5.6%。 相似文献
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Based upon the conception of the least loop,the most loop and direction factor presented by the author firstly,the algorithm of searching out the least and most loops has been designed.By building the adjoining matrix, confirming the start-point of searching,vectorizing the searching sides,calculating the direction factor of adjoining sides,we can search out the next side of the least loop or the most loop rapidly.With a valid searching side being confirmed at every searching and the searching scope being reduced by forming degenerate graph,the time compexity of the algorithm is much less. In the research of the automatic calculating system of construction quantities, the problem of houses partition and out-wall partition had been solved succesfully by utilizing this searching algorithm. 相似文献