副溶血弧菌基因分型和检测的研究进展

副溶血弧菌（Vibrio Parabaemolyticus，VP）是一种革兰氏阴性嗜盐杆菌，是引起食源性疾病的重要病原之一，可导致患者腹泻、肠痉挛、恶心、呕吐、发烧等典型胃肠炎反应。1998年以来的资料显示，副溶血弧菌引发的食物中毒的发生规模及人群暴露规模呈明显上升趋势，均已超过沙门氏菌食物中毒，跃居首位。为了便于进行临床诊断、流行病学调查、食品中微生物检测及医院感染监控，微生物的分型与诊断技术是必不可少的工具。随着分子生物学技术的发展，基因分型与诊断方法以其敏感性高、特异性强、分型率和分辨率佳的优点，逐渐取代传统的表型分型技术，在微生物的分型与诊断中发挥巨大的作用。本文就近年来国内外在副溶血弧菌的基因分型与检测方法上的研究作一综述。     

Iodoacetamide attenuates cognition impairment and amyloid deposit in an AD rat model

AIM:To investigate the effect of an insulin-degrading enzyme (IDE) activator, iodoacetamide, on an AD rat model. METHODS:Thirty-two male Wistar rats were divided into 4 groups:Aβ group, Aβ+ iodoacetamide group, iodoacetamide group and control. Each group included 8 subjects. Aβ was injected into the CA3 area of bilateral hippocampi;Iodoacetamide was administered subcutaneously daily for 5 consecutive days before surgery. The behavior performance in the morris water maze was obtained. The level of Aβ was detected by immunohistochemistry staining and ELISA assay. RESULTS:Level of Aβ was significantly reduced in Aβ+iodoacetamide group compared with that of Aβ group (dropped by 24%), and the Aβ deposit decreased (by 23.9%), the cognition function of this group also greatly improved according to the behavior performance in the morris water maze. The mean escape latency in the place navigation was much lower compared to Aβ group and the times that rats acrossed the central platform in the spatial probe test was also elevated. CONCLUSION:Iodoacetamide significantly reduces the Aβ deposit in the hippocampus in an AD rat model and relieves memory loss.     

应用尖孢镰刀菌毒素滤液鉴定大豆品种抗病性初探

引起大豆根腐病的尖孢镰刀菌粗毒素不仅能使大豆根部产生类似病菌侵染引起的症状,而且能抑制大豆幼根的生长和对大豆幼苗具有致萎作用;用毒素滤液处理大豆胚根和幼根,测定大豆不同品种抗病性,用盆栽土壤作对照,试验结果基本一致。利用毒素滤液测定不同品种对大豆根腐病的抗病性,可应用于大量的初筛工作。     

无子沙糖橘(十月橘)的选育

采用芽变选种的方法,在四会市石狗镇选出了无子柑橘新品种—无子沙糖橘(十月橘)。果略小,单果重平均40g左右,单果平均种子数0.5粒以内,达到柑橘无子的标准。果实扁平,皮橘红色,薄而脆;果肉橙黄色,味清甜,可溶性固形物13%以上,肉质爽脆化渣,品质上等。早结丰产性好,发梢能力强,3月上中旬开花,12月上旬果实开始成熟。     

应用轻型飞机防治马尾松毛虫的试验Ⅰ．喷施四种药剂防治的效果

马尾松毛虫Dendrolimus punctatus Walker是马尾松林重要害虫。林间应用超轻型飞机大面积喷洒溴氰菊酯超低容量喷雾、白僵菌纯孢子油剂、菌药混合液超低容量喷雾以及喷洒白僵菌菌粉,其防治效果分别为96．5%、85．7%、95．6%、86．5%。上述的防治措施可作为单独的措施或综合防控中的配套措施使用,同时应用飞机防治解决了树高、林密、水源缺乏、劳力工资昂贵等条件给防治工作带来的困难,是一种适应林区林业生产整体水平而又快速、高效、经济的防治手段。     

气相色谱法测定咸鱼中的敌百虫和敌敌畏

建立了咸鱼中敌百虫、敌敌畏农药残留的气相色谱分析方法。样品采用乙腈-水(1 1)溶液提取,加乙酸锌净化,用乙酸乙酯进行液液分配,最后采用DB-1701P或DB-5毛细管柱分离,使用气相色谱-火焰光度检测器分析。在4个添加水平的敌百虫、敌敌畏平均回收率(n=6)分别为91%~95%、90%~94.5%,相对标准偏差分别为3.82%~5.80%和3.57%~4.62%;方法的检出限为:敌百虫0.005 mg/kg,敌敌畏0.005 mg/kg。该方法可用于市场上咸鱼样品中敌百虫、敌敌畏的测定。     

Influences of coculture with bone marrow mesenchymal stem cells on dopaminergic neurons in brain slice treated with 1-methyl-4-phenylpytidinium

AIM: To study the protective effects of bone marrow-derived mesenchymal stem cells on damaged dopaminergic neurons induced by 1-methyl-4-phenylpytidinium(MPP+).METHODS: The parkinson disease(PD) models were established in newborn rats. Bone marrow mesenchymal stem cells(MSCs) obtained from adult bone marrow were cultured, isolated and purified. MSCs were co-cultured with brain slice and the immunohistochemical technique, electron microscopy, propidium iodide staining were used to observe the changes of neurons. RESULTS: In the MPP+ treatment group, the neurites grew slowly and sparsely, dead cells were found in all regions. In the co-culture group, the neuritis grew densely, only a few cells were dead, the number of tyrosine hydroxylase(TH)-stained neurons increased and the structure of organellae was normal. CONCLUSION: MSCs may protect dopaminergic neurons against damage induced by MPP+. These results provide some data for cell transplantation therapy to Parkinsons disease.