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131.
132.
Changaram Venugopal Nithya Mariappan Earnestine Holmes Catherine Koch Joseph Francis Susan Eades 《Journal of Equine Veterinary Science》2011,31(12):732-737
Endothelin-1 (ET-1), a physiological as well as an inflammatory mediator, causes contraction of airway smooth muscles by binding to endothelin-A (ETA) and endothelin-B (ETB) receptors. We investigated the interaction between endothelin receptors on contractions and oxidative stress of bronchial rings of clinically healthy horses. Rings for response studies were set in tissue chambers containing oxygenated Tyrode’s solution. Concentration-response relationships were determined for ET-1 on control rings, rings incubated with ETA antagonist (BQ123), rings incubated with ETB antagonists (IRL 1038 and BQ788), and rings incubated with all antagonists combined. For oxidative stress studies, rings were incubated for 30 minutes separately with ET-1, ETA antagonist + ET-1, ETB antagonists + ET-1, and all three antagonists + ET-1. The control rings were not treated with any agents. Electron paramagnetic resonance (EPR) method was used to measure total reactive oxygen species (ROS), superoxide, and peroxynitrite. Results showed that ET-1 caused dose-dependent contractions which were increased by ETA blockade and decreased by ETB blockade. Combined blockade significantly inhibited the response to ET-1. Regarding the oxidative stress, ET-1 and its antagonists significantly reduced the total ROS. Superoxide production was significantly decreased by ETA antagonist and completely abolished when ETA and ETB antagonists were combined. Peroxynitrite production was not changed significantly. The study suggested that an interaction between ET receptors exists in the expression of contractile responses and oxidative stress. ET-1 attenuated oxidative stress by decreasing total ROS. ETA receptors may be primarily responsible for superoxide production. It appears that ET-1 does not affect peroxynitrite production in normal equine tissues. 相似文献
133.
Mbatia B Kaki SS Mattiasson B Mulaa F Adlercreutz P 《Journal of agricultural and food chemistry》2011,59(13):7021-7027
Lipase-catalyzed synthesis of lipophilic phenolic antioxidants was carried out with a concentrate of n-3 polyunsaturated fatty acids (PUFAs), recovered from oil extracted from salmon ( Salmon salar ) byproduct. Vanillyl alcohol and rutin were selected for the esterification reaction, and obtained esters yields were 60 and 30%, respectively. The antioxidant activities of the esters were compared with those of commercial butylated hydroxytoluene (BHT) and α-tocopherol using DPPH radical scavenging and thiobarbituric acid assays. In the DPPH assay, rutin esters showed better activity than vanillyl esters, and on the contrary in lipophilic medium, vanillyl esters were found to be superior to rutin esters. In bulk oil system, the antioxidant activities of rutin and vanillyl derivatives were lower than that of BHT and α-tocopherol, but in emulsion, they showed better activity than α-tocopherol. By attaching to natural phenolics, the PUFAs are protected against oxidation, and PUFA improves the hydrophobicity of the phenolic, which could enhance its function in lipid systems. 相似文献
134.
Biffa D Inangolet F Bogale A Oloya J Djønne B Skjerve E 《Tropical animal health and production》2011,43(2):529-538
This study was aimed at investigating risk factors associated with prevalence of tuberculosis (TB)-like lesions and associated
mycobacteria in Ethiopian cattle slaughtered. The study was carried out during 2006–2007 in five selected municipal and export
abattoirs. Methods of investigation involved detailed necropsy examination of carcasses and isolation of mycobacteria from
pathologic tissue specimens. Factors of animal and environment origin were recorded and examined as explanatory variables
in relation to the presence or absence of TB-like lesions and the presence of viable mycobacteria. Multivariable logistic
regression analysis was used to identify risk factors associated with prevalence of TB-like lesions and mycobacteria. Out
of 3,322 carcasses inspected, 10.2% (95% confidence interval (CI) [9.2–11.3]) and 3.2% (95% CI [2.6–3.8]) were positive, respectively,
based on necropsy and bacteriologic examinations. The highest and lowest lesion prevalence was recorded in Adama (24.7%) and
Yabello (4.2%), respectively. Multivariable logistic regression analysis identified age, breed, abattoir location, geographic
origin and management system as being risk factors for prevalence of TB-like lesions and occurrence of viable mycobacteria
in Ethiopian cattle. The study demonstrated mycobacterial infection as important infectious disease of Ethiopian cattle. The
reported confirmed cases of the disease in different management and geographic settings in Ethiopia disproved the earlier
held opinion of its occurrence as a low sporadic profile. In view of a dietary proclivity of Ethiopian communities (milk and
meat are predominantly consumed raw) and lifestyle (close contact of people with animals), the risk of bovine tuberculosis
as a public health threat is eminent. 相似文献
135.
136.
137.
Jalal Jamalian Francis Aylward Bertram J. F. Hudson 《Plant foods for human nutrition (Dordrecht, Netherlands)》1977,27(2):213-219
Ingestion of broad bean seeds will precipitate an acute haemolytic anaemia (favism) in certain sensitive subjects. Aqueous extracts from both fresh and dried mature broad bean seeds, obtained from a favism-endemic area in Iran, were incubated with blood from favism-sensitive and control subjects. Changes in the reduced glutathione content of red blood cells were determined as an index of toxicity. The results showed that:
- The toxic principles in broad beans were at least partially water soluble.
- The seed coats of dry mature seeds and the flesh from fresh seeds had low toxicity.
- The immature whole seeds and the seed coats of fresh mature seeds were toxic.
138.
J. Bota M. À. Conesa J. M. Ochogavia H. Medrano D. M. Francis J. Cifre 《Genetic Resources and Crop Evolution》2014,61(6):1131-1146
Tomàtiga de Ramellet (Ramellet) is a traditional long-storage tomato from the Mediterranean Islands of Mallorca, Menorca, Eivissa (Ibiza), and Formentera (Balearic Islands). To investigate the observed phenotypic and shelf life variability within local varieties, we studied 142 accessions characterized as Ramellet varieties, and 29 accessions representing other local varieties. The 171 accessions constitute a new germplasm collection at the University of Balearic Islands (UIB). The evaluation of the collection demonstrated wide variability in most agronomic, morphological and quality traits of Ramellet accessions and clearly differentiates them from other local varieties with respect to fruit shape, size, fruit composition, and fruit storage. All accessions of Ramellet evaluated have the ripening mutation alcobaça (alc), suggesting an important role for this variant in controlling shelf life. Results of evaluation also showed wide variability between the Ramellet accessions for storage time, suggesting that other traits like fruit size and composition may play a role in fruit conservation. Observed variation within the Ramellet accessions is consistent with a genetically variable population or landrace group. The establishment of this collection will help prevent genetic erosion of this local landrace group and constitute a resource for future investigation and breeding. 相似文献
139.
Robert L. Metcalf B.Magnus Francis Robert A. Metcalf Larry G. Hansen 《Pesticide biochemistry and physiology》1983,20(1):57-66
Nineteen O-halogenated-phenyl O-methyl phenylphosphonothionates were evaluated for acute toxicity (LD50) to the female house fly Musca domestica L. and to the male Swiss white mouse, and for delayed neurotoxicity to the White Leghorn hen. The electron-withdrawing power of the phenyl substituents (Σσ? values) correlate with the LD50 values to house fly and mouse, with departures from linearity attributable to the steric hindrance of di-ortho-Cl substitution and by variations in the accessibility of the anionic site of acetylcholinesterase in the two species. The relationship with delayed neurotoxicity is less predictable although it clearly depends on suitable electron-withdrawing capacity. Delayed neurotoxicity also relates to a high degree of lipophilicity and prolonged residence time of the inhibitor in the nerve axon. 相似文献
140.
B H Janke D H Francis J E Collins M C Libal D H Zeman D D Johnson 《Journal of veterinary diagnostic investigation》1989,1(1):6-11
Attaching and effacing Escherichia coli (AEEC) adhere to mucosal epithelium in both small and large intestine and induce a distinctive lesion characterized by an irregular scalloped appearance of the epithelial layer. Infection with attaching and effacing E. coli was detected in 14 calves, 7 pigs, 2 lambs, and 3 dogs. Affected animals were from farms and kennels in South Dakota, Minnesota, Iowa, Nebraska, and Wisconsin. Ages of affected animals were calves, 2 days to 4 months; pigs, 1-6 weeks; lambs, 1 week; and dogs, 7-8 weeks. Clinical signs included diarrhea in all animals, but other nonenteric disease problems were present in some animals. Concurrent infection with other enteropathogens was detected in 9 calves and 5 pigs. Infection with AEEC appeared to be the sole cause of illness and death in some animals. There was evidence of intestinal hemorrhage in 5 of the calves and in all 3 dogs. Attaching and effacing lesions varied from small scattered foci to widespread involvement of large areas of intestinal mucosa. Verotoxin was produced by E. coli strains isolated from 9 calves, but not by strains from pigs, lambs, or dogs. 相似文献