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41.
Sánchez AM Carmona M Zalacain A Carot JM Jabaloyes JM Alonso GL 《Journal of agricultural and food chemistry》2008,56(9):3167-3175
The aim of this work was the development of multivariate models able to determine the content of the main crocetin esters and picrocrocin from spectrophotometric data that could be used for routine quality control of saffron. These compounds were determined with HPLC in Spanish saffron, and their absorbance spectra from 190 to 700 nm were simultaneously monitored. Partial least-squares regression (PLSR) models have been obtained and applied to the determination of individual crocetin esters, to the sum of crocetin esters, and to picrocrocin. A modification of the Kennard-Stone algorithm was used to divide the pool of samples into calibration and validation subsets. The best predictions were obtained with the sum of crocetin esters model, followed by the model for cis-crocetin (beta- D-glucosyl)-(beta- D-gentiobiosyl) ester, trans-crocetin di-(beta- D-gentiobiosyl) ester, and trans-crocetin (beta- D-glucosyl)-(beta- D-gentiobiosyl) ester, whereas the worst predictions were found with the picrocrocin and trans-crocetin (beta- D-gentiobiosyl) ester models. These models may enhance quality control in saffron enterprises. 相似文献
42.
43.
Systematic HPLC analysis of aqueous extracts from bark and woody parts of Quillaja saponaria Mol. ('quillay') growing at three different localities in Central Chile, showed that saponin contents in 100 g of dried plant material ranged from 2.3 g to 15.8 g depending on the extraction method (soxhlet or maceration), the part of the plant analysed and the site of collection. Twigs and barks afforded in most cases extracts with higher levels of saponins as compared to those obtained with woody parts of the same specimen. 相似文献
44.
Structural changes of hake (Merluccius merluccius L.) fillets: effects of freezing and frozen storage. 总被引:1,自引:0,他引:1
M Careche A M Herrero A Rodriguez-Casado M L Del Mazo P Carmona 《Journal of agricultural and food chemistry》1999,47(3):952-959
Structural changes in hake (Merluccius merluccius L.) fillets as affected by freezing method and frozen storage temperature have been studied through Raman spectroscopy and related to changes in texture and functionality. Changes in protein secondary structure were observed due to storage temperature, accompanied by changes in apparent viscosity and shear resistance. Samples at -10 degrees C showed greater structural alteration than at -30 degrees C in terms of increase of beta-sheets at the expense of alpha-helices. An increase of unordered protein structure was found only in samples stored at -10 degrees C. Exposure of buried tryptophan residues was observed at both storage temperatures. The decrease of the deltaCH(2) band upon storage suggested an increase of hydrophobic interactions of aliphatic residues. Except for liquid air frozen fillets, all samples showed a decrease of the nuO-H/nuC-H band ratio compared to the fresh ones, this decrease being higher the harsher the conditions. 相似文献
45.
Héctor Mendoza Guzmán Adelina de la Jara Valido Laura Carmona Duarte Karen Freijanes Presmanes 《Aquaculture International》2010,18(2):189-199
The present work shows the possibility of determining variations in the lipid composition in Tetraselmis suecica under different conditions of culture by means of flow cytometry in cells marked with Nile Red (NR). A significant correlation
was observed between the cellular contents in polar and neutral lipids and the cytometric signal of the marked cells. Likewise,
there was a significant correlation between the ratio of polar and neutral lipids, estimated by cytometry, and the relative
composition of polyunsaturated fatty acids (PUFAs) in Tetraselmis, which corresponded to the greater content of PUFAs detected in the polar lipid fraction of this microalgae. This relationship
between the polar/neutral ratio and the relative contents of PUFAs, together with the flow cytometry and the marking by means
of NR, would make it possible to have an effective indicator of the abundance of PUFAs in Tetraselmis, as well as the development of techniques of massive screening of strains which are hyperproductive of PUFAs and of rapid
checking of the variations in lipid composition in response to cultivation conditions, which are much simpler and more rapid
than traditional techniques. 相似文献
46.
Soil erosion and runoff in different vegetation patches from semiarid Central Mexico 总被引:2,自引:0,他引:2
Rebeca Vásquez-Méndez Eusebio Ventura-Ramos Klaudia Oleschko Luis Hernández-Sandoval Jean-Francois Parrot Mark A. Nearing 《CATENA》2010
Vegetation patches in arid and semiarid areas are important in the regulation of surface hydrological processes. Canopy and ground covers developed in these fertility islands are a natural cushion against the impact energy of rainfall. Also, greater levels of organic matter improve the soil physicochemical properties, promoting infiltration and reducing runoff and soil erosion in comparison with the open spaces between them. During the 2006 rainy season, four USLE-type plots were installed around representative vegetation patches with predominant individual species of Huisache (Acacia sp), Mesquite (Prosopis sp), Prickly Pear or Nopal (Opuntia sp) and Cardon (Opuntia imbricata), to evaluate soil erosion and runoff, in semiarid Central Mexico. A comparative bare surface condition (Control) was also evaluated. Vegetative canopy and ground cover were computed using digital images. Selected soil parameters were determined. Soil erosion was different for the studied vegetation conditions, decreasing as canopy and ground cover increased. There were not significant differences in runoff and soil erosion between the Control and O.imbricata surfaces. Runoff was reduced by 87%, 87% and 98% and soil loss by 97%, 93%, and 99% for Acacia farnesiana, Prosopis laevigata and Opuntia sp, respectively, as compared to the Control. Soil surface physical conditions were different between the low vegetation cover conditions (Control and O.imbricata surfaces) and the greater vegetation cover conditions (A.farnesiana, P.laevigata and Opuntia sp), indicating a positive effect of vegetation patches on the regulation of surface hydrological processes. 相似文献
47.
Carmona M Zalacain A Salinas MR Alonso GL 《Journal of agricultural and food chemistry》2006,54(18):6825-6834
Generation of volatiles by thermal treatments has been studied in saffron spice for two reasons: (a) to determine volatile profile changes during simulated aging processes and (b) to study the volatile generation pathway. During the aging process, while the amounts of C10 compounds such as safranal and HTCC increase, the amounts of C9 compounds such as isophorone and 2,6,6-trimethylcyclohexane-1,4-dione decrease. A new compound tentatively identified as 4,5,6,7-tetrahydro-7,7-dimethyl-5-oxo-3H-isobenzofuranone seems to play a very important role in the aging process. The importance of this compound, structurally similar to dihydroactindiolide, was also confirmed when the saffron volatile fraction was analyzed via the degradation of the linear chain of crocetin and crocetin esters and is reported for the first time in this paper. Thermal degradation studies of zeaxanthin, crocetin, and trans and cis crocetin esters isomers allowed us to propose different mechanisms which explain saffron volatile generation depending on the crocetin ester isomer structure. 相似文献
48.
Costa MF Ronchi FA Ivanow A Carmona AK Casarini D Slocombe RF 《Research in veterinary science》2012,93(2):993-994
Exercise-induced pulmonary haemorrhage has an impact on racehorse performance. Although endoscopic diagnosis (with or without the aid of bronchoalveolar lavage) is considered to be the standard diagnostic method for this condition, the use of biomarkers that could aid in quantifying risk and severity of the condition would represent an advance in equine sport medicine. This preliminary research investigated the use of angiotensin-converting enzyme (ACE) activity in plasma of racehorses and demonstrated that ACE activity is increased in horses with higher degrees of haemorrhage and is a promising biomarker for EIPH in racehorses. 相似文献
49.
Erreguerena Ignacio Antonio Havis Neil David Heick Thies Marten Gorniak Kalina Quiroz Facundo Carmona Marcelo Anibal 《植物病害和植物保护杂志》2022,129(6):1343-1353
Journal of Plant Diseases and Protection - Ramularia leaf spot (RLS) caused by the fungus Ramularia collo-cygni (Rcc) has become a threat to barley production in Argentina. All barley varieties are... 相似文献
50.
Characterization of catecholase and cresolase activities of eggplant polyphenol oxidase 总被引:4,自引:0,他引:4
In the present paper the catecholase and cresolase activities of eggplant polyphenol oxidase (PPO) are described. To preserve the latter activity, a partially purified enzyme was used. Peroxidase was removed from the preparation to avoid its interference with PPO during phenol oxidation. The partially purified eggplant PPO was fully active. The catecholase/cresolase ratio of 41.1 indicated that, in a pH close to the physiological, diphenol oxidation predominates over monophenol oxidation. The characteristic lag phase of the cresolase activity is modulated by the pH, the monophenol and diphenol concentrations, and the enzyme's concentration. The effect of several inhibitors was also tested, and the K(i) values of the two most effective (tropolone and 4-hexylresorcinol) were determined. 相似文献