Detection of ruminant meat and bone meals in animal feed by real-time polymerase chain reaction: result of an interlaboratory study

The commercialization of animal feeds infected by prions proved to be the main cause of transmission of bovine spongiform encephalopathy (BSE). Therefore, feed bans were enforced, initially for ruminant feeds, and later for all feeds for farmed animals. The development and validation of analytical methods for the species-specific detection of animal proteins in animal feed has been indicated in the TSE (Transmissible Spongiform Encephalopathies) Roadmap (European Commission. The TSE (Transmissible Spongiform Encephalopathy) roadmap. URL: http://europa.eu.int/comm/food/food/biosafety/bse/roadmap_en.pdf, 2005) as the main condition for lifting the extended feed ban. Methods based on polymerase chain reaction (PCR) seem to be a promising solution for this aim. The main objective of this study was to determine the applicability of four different real-time PCR methods, developed by three National expert laboratories from the European Union (EU), for the detection and identification of cattle or ruminant species in typical compound feeds, fortified with meat and bone meals (MBM) from different animal species at different concentration levels. The MBM samples utilized in this study have been treated using the sterilization condition mandatory within the European Union (steam pressure sterilization at 133 degrees C, 3 bar, and 20 min), which is an additional challenge to the PCR methods evaluated in this study. The results indicate that the three labs applying their PCR methods were able to detect 0.1% of cattle MBM, either alone or in mixtures with different materials such as fishmeal, which demonstrates the improvement made by this technique, especially when compared with results from former interlaboratory studies.     

Phylogenetic analysis of the SSU rRNA gene from the piscine diplomonad Spironucleus torosus (Diplomonadida: Hexamitinae)

Previous studies have recorded Spironucleus torosus Poynton et Morrison, 1990 from several species of gadoid fishes, including the only freshwater gadoid, the burbot Lota lota (L.). Two morphologically different isolates of S. torosus have been described (elongate and pyriform). Both have been found in saltwater, while only the elongate has been found in freshwater. To address the conspecificity of the two morphs of S. torosus, and to identify the source of S. torosus in burbot in Norway, we have sequenced the small subunit ribosomal RNA (SSU rRNA) gene from 43 isolates of S. torosus from six species of gadoid fishes sampled at 15 localities in Norway, Sweden and the Baltic Sea. Phylogenetic analyses of the SSU rRNA gene sequence data recovered two major clades, one containing mainly isolates from burbot, while the other contained isolates from marine gadoid fishes only. The genetic distance (based on 25 nucleotide substitutions in 789 base pairs) separating the two assemblages was not large enough to consider the two groups separate species. Spironucleus torosus isolated from burbot displayed limited genetic variation in the small subunit ribosomal RNA (SSU rRNA) gene along the post-Pleistocene migration route of its host. The present study is the first report of S. torosus in tusk Brosme brosme (Ascanius), whiting Merlangius merlangus (L.), and fourbeard rockling Enchelyopus cimbrius (L.).     

Dynamics and drivers of aboveground biomass accumulation during recovery from selective harvesting in an uneven-aged forest

European Journal of Forest Research - Selective harvesting is a common silvicultural practice to ensure sustainable use of uneven-aged forests, but our understanding of how multiple forest...     

German farmers’ intention to use autonomous field robots: a PLS-analysis

Autonomous field robots are a promising technology for solving several problems in agriculture, as they are electrical driven, can control weeds single-plant based mechanically or with microdoses of pesticides and exert less ground pressure on the field. Whether such robots will be applied on a large scale in German agriculture depends on various parameters. Therefore, the factors influencing the behavioural intention of farmers with respect to their future adoption of autonomous field robots were investigated. The analysis applies a structural equation model based on an extended version of the Unified Theory of Acceptance and Use of Technology. The dataset, collected in 2019, consists of 500 German farmers. The results reveal significantly positive effects of farmers’ expected performance, social influence and trust as well as significantly negative effects of farmers’ effort expectancy and anxiety on the behavioural intention to use autonomous field robots. Additionally, moderating effects of age on the relationship of individual constructs to the behavioural intent to use robots could be confirmed. The results provide important information for various stakeholders. Robot suppliers should better inform farmers about the performance of their products, for instance by involving farmers in the development process of the robots. The ecological benefits attributed to field robots could meet public expectations and should be better communicated to address farmers’ social influence on the behavioural intention to use the robots. Policymakers could try to create better framework conditions, for example by establishing a stable legal situation for autonomous systems or promote its use.