广州市公益林生态效益价值分析及管理对策

在对广州市公益林分布和现状特点分析的基础上 ,从林种的生态功能角度对效益进行了分析。结果表明 :广州市水源涵养林生态效益比较高 ,生态效益从大到小排序为 :水源涵养林 >水土保持林 >风景林、自然保护区和小片林 >护岸林 ,从单个效益分析 ,维持大气平衡效益最大。最后提出了公益林管理对策     

新亚欧大陆桥经济带沙区土地利用及环境变迁的时空分析模型

本文分析了新亚欧大陆桥经济带及其沙区的发展问题，介绍了国内外对沙漠土地的研究与利用情况，提出了新亚欧大陆桥沙区的经济模式和土地利用及环境变迁的时空分析模型。     

Cytotoxic activity of the root extract from Myoschilos oblongum

The crude methanolic root extract of Myoschilos oblongum exhibited a significant cytotoxic activity against PZ-HPV-7 human prostate cells. Furthermore, two esters of docosanol were isolated from the CH(2)Cl(2) extract.     

DIAGNOSTIC SENSITIVITY OF ULTRASOUND,RADIOGRAPHY AND COMPUTED TOMOGRAPHY FOR GENDER DETERMINATION IN FOUR SPECIES OF LIZARDS

Gender determination is frequently requested by reptile breeders, especially for species with poor or absent sexual dimorphism. The aims of the current study were to describe techniques and diagnostic sensitivities of ultrasound, radiography, and computed tomography for gender determination (identification of hemipenes) in four species of lizards. Nineteen lizards of known sex, belonging to four different species (Pogona vitticeps, Uromastyx aegyptia, Tiliqua scincoides, Gerrhosaurus major) were prospectively enrolled. With informed owner consent, ultrasound, noncontrast CT, contrast radiography, and contrast CT (with contrast medium administered into the cloaca) were performed in conscious animals. Imaging studies were reviewed by three different operators, each unaware of the gender of the animals and of the results of the other techniques. The lizard was classified as a male when hemipenes were identified. Nineteen lizards were included in the study, 10 females and nine males. The hemipenes were seen on ultrasound in only two male lizards, and appeared as oval hypoechoic structures. Radiographically, hemipenes filled with contrast medium appeared as spindle‐shaped opacities. Noncontrast CT identified hemipenes in only two lizards, and these appeared as spindle‐shaped kinked structures with hyperattenuating content consistent with smegma. Hemipenes were correctly identified in all nine males using contrast CT (accuracy of 100%). Accuracy of contrast radiography was excellent (94.7%). Accuracy of ultrasound and of noncontrast CT was poor (64.3% and 63.1%, respectively). Findings from the current study supported the use of contrast CT or contrast radiography for gender determination in lizards.     

Relationship between three novel SNPs of BRCA1 and canine mammary tumors

The BRCA1 gene plays an important role in the development of human breast cancer, and recent research indicated that genetic variations of BRCA1 are also related to canine mammary tumors (CMTs). Here, using rapid amplification of cDNA ends (RACE), we cloned the 5′- and 3′-UTRs of BRCA1. By direct sequencing of the flanking sequences of the 5′- and 3′-UTRs of BRCA1, three previously unreported single-nucleotide polymorphisms (SNPs) were identified, two (−1228T >C, −1173C >T) in the putative promoter regions and one non-synonymous SNP (63449G >A) in exon 23. Compared with 16 normal samples, the sequences from 34 CMTs suggested that SNP (−1173C >T) was associated with the development of CMTs (odds ratio (OR)=2.57, 95% confidence interval (CI): 1.07–6.15).     

Influence of race and crossbreeding on casein micelles size

Casein (CN) micelles are colloidal aggregates of protein dispersed in milk, the importance of which in the dairy industry is related to functionality and yield in dairy products. The objective of this work was to investigate the correlation of milk CN micelles diameter from Holstein and Zebu crossbreds with milk composition (protein, fat, lactose, total and nonfat solids and milk urea nitrogen), somatic cell count (SCC), age, lactation stage and production. Average casein micelles diameters of milk samples obtained from 200 cows were measured using photon correlation spectroscopy and multiple regression analysis was used to find relationship between variables. CN micelle diameter, SCC and nonfat solids were different between animals with different Holstein crossbreed ratios, which suggests influence of genetic factors, mammary gland health and milk composition. Overall, results indicate the potential use of CN micelle diameter as a tool to select animals to produce milk more suitable to cheese production.     

关岭牛MyoD Ⅰ基因在不同组织和时期中的表达分析

为研究MyoDⅠ基因在关岭牛不同组织表达量的差异及在肌肉发育过程中的表达情况,试验采用实时荧光定量PCR技术对关岭牛背最长肌、大腿肌、心脏、肝脏、脂肪、小肠6个组织的MyoDⅠ基因相对表达量进行检测。同时分析关岭牛胎儿、18月龄、30月龄时期背最长肌组织中MyoDⅠ基因的表达规律。结果显示,MyoDⅠ基因在背最长肌中表达量最高,在出生后的关岭牛肌肉组织中也具有较高的表达量,且随着出生时间的推移呈上升趋势。推测可能由于背最长肌是肌肉富集地方,因此基因表达量较高,随着年龄增大,肌肉丰满度增加,基因表达量也随着增加。     

曲轴摆缸式阀配流水液压马达工作特性研究

为解决盘配流和轴配流低速大扭矩水液压马达配流副存在的磨损和泄漏问题,提出一种新型阀配流结构的低速大扭矩水液压马达,柱塞配流通过配流凸轮控制配流阀的通断实现。研究柱塞运动学规律和马达输出扭矩形成,揭示配流阀推杆位移、阀芯通断、柱塞进回液间的对应关系,分析马达角排量波动随转角、结构设计参数K的变化规律,当结构设计参数K为0.13时,马达输出扭矩波动率为6.59%。在AMESim中建立了配流阀及单柱塞配流过程的仿真模型,分析配流阀的工作特性及单柱塞动态配流性能,工作中配流阀产生最大压降为0.08MPa,进/回液配流阀无高低压串液。为验证阀配流结构在水液压马达中的工作性能,建立阀配流单柱塞试验台,并研究不同工况下的配流工作特性。试验结果表明,配流阀在马达转速0~60r/min、压力0~21MPa的工况下稳定配流,进液阀口压力在柱塞腔进回液转换时存在瞬间小幅压力波动,但对配流过程基本无影响,阀配流结构能够满足曲轴摆缸式马达柱塞的配流需求,为低速大扭矩水液压马达的配流提供了思路。