Determination of evapotranspiration for maize and berseem clover

Maize and berseem are among the most important crops in India and several other countries in the world. Irrigation is provided to these crops to get higher production; hence, determining the water requirements of these crops is important for irrigation planning. Improved water management of these crops requires accurate scheduling of irrigation, which in turn requires accurate measurement of crop evapotranspiration (ET_c). Thus, the first objective of this study was to measure daily, weekly and seasonal ET_c of maize and berseem directly from weighing type lysimeters. Experiments were conducted in a set of two electronic weighing-type lysimeters of 7.82 m³ to measure the hourly ET_c of maize and berseem from June 1996 to April 1998 at Karnal, India. The average daily ET_c of maize varied from <2.8 mm day^-1 in the early growing period to >4 mm day^-1 at development and reproductive stages. The peak daily ET_c of maize was 7.7 mm day^-1 and this occurred 9 weeks after sowing (WAS) at the silking stage of maize when leaf area index (LAI) was 5.5. The measured seasonal ET_c of maize was 354 mm. In the case of berseem, the average daily ET_c was 0.9 mm day^-1 at the initial stage, achieved a peak value of 6.9 mm day^-1 between 25 and 26 WAS during the fifth cut. The measured seasonal ET_c of berseem was 480 mm. Precise information on the crop coefficient, which is required for regional-scale irrigation planning, is lacking for semi-arid climates such as those found in north India. Therefore, the second objective of this study was to develop crop coefficients (K_c) for maize and berseem from ET_c measurements and weather data. The estimated values of K_c for maize by the Penman-Monteith method at the four crop growth stages; namely, initial, crop development, mid-season and maturity, were 0.55, 1.00, 1.23 and 0.64, respectively, and the corresponding values for berseem were 0.76, 0.82, 1.11 and 1.24, respectively. In the case of these two crops, actual K_c values determined from this study are different from those suggested by the FAO (Allen et al. 1998), indicating the need for generating these values at the local/ regional level.     

Differential proteins associated with plasma membrane in X- and/or Y-chromosome bearing spermatozoa in indicus cattle

The differential proteins associated with plasma membrane of spermatozoa are less known, identification of which shall help overcome limitations of currently used methods of sperm sexing, considered as a high priority for livestock sector of many countries. This study has reported plasma membrane proteomics of unsorted spermatozoa and differential expression of plasma membrane-associated proteins between X- and Y-chromosome bearing spermatozoa of indicus cattle (Bos indicus). Isolation of plasma membrane fraction using percoll gradient, relatively a rapid method, from bovine spermatozoa has been reported to enrich isolation of plasma membrane proteins. Significant enrichment for plasma membrane-associated proteins was observed in plasma membrane fraction (p < .05) as compared to the total cell lysate using LC-MS/MS. Furthermore, these experiments were conducted in flow cytometry sorted, sexed-semen samples. Thirteen proteins were identified as differentially abundant between X- and Y-sorted spermatozoa. Among these, two proteins were downregulated in Y-sorted spermatozoa compared to the X-sorted spermatozoa (p < .05), while four and seven proteins could be noted in X- and Y-sorted spermatozoa, respectively. Proteins that are presumed to support sperm capacitation and sperm migration velocity were found to be abundant in Y-sorted spermatozoa while those associated with structural molecule activity were identified as abundant in X-sorted spermatozoa in the present study. Our study provides better insight into the plasma membrane proteomics of spermatozoa of indicus cattle and furnishes data that might aid in design and development of alternate and open technology for sex-sorting of semen.     

Flavoring components of raw monsooned arabica coffee and their changes during radiation processing

Volatile aroma principles, nonvolatile taste constituents (caffeine and chlorogenic and caffeic acids), and glycosidically bound aroma compounds of monsooned and nonmonsooned raw arabica coffee were analyzed using gas chromatography-mass spectrometry (GC-MS) and high-performance liquid chromatography (HPLC). Among the most potent odor active constituents known to contribute to the aroma of the green beans, 3-isopropyl-2-methoxypyrazine, 3-isobutyl-2-methoxypyrazine, 4-vinylguaiacol, beta-damascenone, (E)-2-nonenal, trans,trans-2,4-decadienal, phenylacetaldehyde, and 3-methylbutyric acid were detected by GC-MS in both samples. A decrease in content of methoxypyrazines and an increase in 4-vinylguaiacol and isoeugenol resulted in a dominant spicy note of monsooned coffee. These phenolic compounds exist partly as their glycosides, and their release from the bound precursors during monsooning accounted for their higher content in monsooned coffee. A considerable decrease in astringent chlorogenic acid as a consequence of hydrolysis to bitter caffeic acid was noted in monsooned coffee. Radiation processing of nonmonsooned beans at a dose of 5 kGy resulted in an increased rate of monsooning. At this dose a quantitative increase in most of the aroma active components could be observed in all samples studied. Hydrolysis of chlorogenic acid to caffeic acid was noted in radiation-processed monsooned coffee beans irrespective of whether the treatment was carried out before or after monsooning. These changes were, however, not observed in irradiated, nonmonsooned coffee beans, suggesting an enzymatic rather than a radiolytic cleavage of chlorogenic acid. A rationale behind the mechanism of monsooning and radiation-induced enhancement of the monsooning process is discussed.     

Endogenous substrate for cyclic AMP-dependent protein kinase in adrenocortical polyadenylated messenger ribonucleoproteins

An endogenous polysomal cyclic AMP-dependent protein kinase specifically phosphorylates a 150,000-dalton peptide bound to an adrenocortical polyadenylated messenger ribonucleoprotein complex. There is a possibility that this protein is a physiological substrate of cyclic AMP-dependent protein kinase and that the phosphorylation and dephosphorylation of this substrate may be important in the translation control of adrenal polyadenylated messenger RNA.     

Horticultural crops tackling stresses: genetic and epigenetic alterations

Genetic Resources and Crop Evolution - Food is indispensable for fulfilling the nutritional requirements of living beings. The principal source of food, nutrition and feed are horticultural...     

Storage changes in the quality of sound and sprouted flour

Sound and sprouted flours (24 and 48 hr) from bread wheat (WL-1562), durum wheat (PBW-34) and triticale (TL-1210) were stored at room temperature (34.8°C) and relative humidity (66.7%) for 0, 45, 90 and 135 days to assess the changes in physico-chemical and baking properties. Protein, gluten, sedimentation value, starch and crude fat decreased during storage in all the samples; however, the decrease was more in sprouted flours. Free amino acids, proteolytic activity, diastatic activity and damaged starch decreased with increase in storage period. Total sugars and free fatty acids increased more rapidly in the flours of sprouted wheats during 135 days of storage. Loaf volume of breads decreased during storage in both sound and sprouted flour but the mean percent decrease in loaf volume was more in stored sound flours. Aging of sprouted flour for 45 days improved the cookie and cake making properties but further storage was of no value for these baked products.Chapati making properties of stored sound and sprouted flour were inferior to that of fresh counterparts.     

Production of alpha 1,3-galactosyltransferase-deficient pigs

The enzyme alpha1,3-galactosyltransferase (alpha1,3GT or GGTA1) synthesizes alpha1,3-galactose (alpha1,3Gal) epitopes (Galalpha1,3Galbeta1,4GlcNAc-R), which are the major xenoantigens causing hyperacute rejection in pig-to-human xenotransplantation. Complete removal of alpha1,3Gal from pig organs is the critical step toward the success of xenotransplantation. We reported earlier the targeted disruption of one allele of the alpha1,3GT gene in cloned pigs. A selection procedure based on a bacterial toxin was used to select for cells in which the second allele of the gene was knocked out. Sequencing analysis demonstrated that knockout of the second allele of the alpha1,3GT gene was caused by a T-to-G single point mutation at the second base of exon 9, which resulted in inactivation of the alpha1,3GT protein. Four healthy alpha1,3GT double-knockout female piglets were produced by three consecutive rounds of cloning. The piglets carrying a point mutation in the alpha1,3GT gene hold significant value, as they would allow production of alpha1,3Gal-deficient pigs free of antibiotic-resistance genes and thus have the potential to make a safer product for human use.     

Productivity of Rice—Wheat Cropping System in a Part of Indo-Gengetic Plain: A Spatial Analysis

Investigations were made to study the effect of unequal distribution of canal water in land and water productivity of the rice—wheat cropping system in terms of head—tail relationship in Bhakra Canal command, Haryana. Information on water supply, agronomic practices, crop yield, etc.,were collected from 216 farmers comprising 36 farmers each from the head, middle, and tail watercourses of two minors during year 2000–01. The unequal supply of canal water and presence of marginal quality groundwater creates large variations in the cropping pattern, irrigation application, and land and water productivity of the irrigation system. The groundwater of tail reaches, being saline in nature, was about 25% less productive as compared to head reaches. The unavailability of canal water in the tail reaches creates more dependency on groundwater. Due to its poor quality the crop production in the tail reaches was less by 10 to 20% in case of wheat, and 20 to 40% in case of rice, as compared to head reaches. Groundwater transfer from head to tail reaches and cultivation of low water requiring salt tolerant crops/varieties would be helpful in reducing the productivity gap and increasing the profitability of the farms in the region.