Landscape- and local-level variables affect monarchs in Midwest grasslands

Landscape Ecology - It is estimated that over one billion milkweed stems need to be restored to sustain the eastern North American migratory population of monarch butterflies; where and in what...     

Comment on "Wetland sedimentation from hurricanes Katrina and Rita"

Turner et al. (Reports, 20 October 2006, p. 449) measured sedimentation from Hurricanes Katrina and Rita in coastal Louisiana and inferred that storm deposition overwhelms direct Mississippi River sediment input. However, their annualized hurricane deposition rate is overestimated, whereas riverine deposition is underestimated by at least an order of magnitude. Their numbers do not provide a credible basis for decisions about coastal restoration.     

Fracture-controlled paleo-fluid flow in Candor Chasma, Mars

Color observations from the High Resolution Imaging Science Experiment on board the Mars Reconnaissance Orbiter reveal zones of localized fluid alteration (cementation and bleaching) along joints within layered deposits in western Candor Chasma, Mars. This fluid alteration occurred within the subsurface in the geologic past and has been exposed at the surface through subsequent erosion. These findings demonstrate that fluid flow along fractures was a mechanism by which subsurface fluids migrated through these layered deposits. Fractured layered deposits are thus promising sites for investigating the geologic history of water on Mars.     

Computation-guided backbone grafting of a discontinuous motif onto a protein scaffold

The manipulation of protein backbone structure to control interaction and function is a challenge for protein engineering. We integrated computational design with experimental selection for grafting the backbone and side chains of a two-segment HIV gp120 epitope, targeted by the cross-neutralizing antibody b12, onto an unrelated scaffold protein. The final scaffolds bound b12 with high specificity and with affinity similar to that of gp120, and crystallographic analysis of a scaffold bound to b12 revealed high structural mimicry of the gp120-b12 complex structure. The method can be generalized to design other functional proteins through backbone grafting.