导叶相位角度对管道输油泵水力性能的影响

为提高管道输油泵水力性能,以某型号大型管道输油泵为研究对象,针对导叶相位角度对其水力性能的影响进行研究.采用Pro/E与Gambit软件对该输油泵全流道内流场进行三维造型与网格划分.应用计算流体动力学软件CFX对输油泵在6个导叶相位(0°,6°,12°,18°,24°和30°)及5个流量工况(0.8Q_d,0.9Q_d,1.0Q_d,1.1Q_d和1.2Q_d)下的内流场进行定常数值计算,得到了不同导叶相位下输油泵的性能曲线,对比分析了不同导叶相位下输油泵的内部流动情况.研究结果表明:不同相位时,在设计工况下扬程差别较大,扬程最大相差6%,大流量及小流量工况下扬程的差别较小;设计工况下效率差别较小,大流量及小流量工况下效率的差别增大,效率最大相差2%;导叶叶片背面出口处的低速区是影响输油泵水力性能的重要因素,不同导叶相位下,蜗壳隔舌处的流场不同,进而影响输油泵的水力性能;受导叶流道出口低速区的影响,导叶相位为6°,12°及18°时,蜗壳扩散段流态较差,导致输油泵效率偏低,且蜗壳出口速度分布非常不均,不利于输油泵高效平稳地运行.     

基于村民参与式的乡村人居环境评价研究

乡村人居环境改善是城乡统筹发展的重要内容,也是美丽乡村建设的关键所在。笔者着眼于“以人为本”的视角,以重庆市铜梁区南城街道鱼溅村为例,通过村民参与式与数学模型定量的研究方法,研究乡村人居环境村民的满意度和优化方向。研究结果显示：鱼溅村人居环境的整体满意度水平一般,人居软环境系统的社会环境与经济环境满意度为一般和不满意,硬环境系统的设施环境和生态环境对整体满意度贡献率较高,结论表明鱼溅村亟需加强对人居软环境系统的优化;同时,研究还显示鱼溅村人居环境满意度的空间分布差异较大,这说明人居环境的优化需要合理布局、整体筹划。笔者最后提出了优化的对策,对山地丘陵地区的乡村人居环境建设具有普遍借鉴意义。     

小麦秆黑粉病持续控制技术研究

小麦秆黑粉病曾是中国北方麦区主要病害,近年病情回升、局部为害严重。为持续控制该病严重流行造成的毁灭性灾害,本研究采用品种抗病性鉴定和药剂多点试验结果多重比较的方法,从目前推广品种（系）资源207 份材料中,鉴选出高抗和中抗品种,‘晋麦31’、‘晋麦32’、‘晋麦33’、‘临优145’、‘临抗20’、‘运旱618’、‘运94-32’、‘豫麦57’、‘烟农19’、‘郑麦9023’、‘冀麦32’等11 个;利用3 地区不同生态条件多重复鉴定分析方法,试验出可替代五氯硝基苯的拌种药剂类型有三唑醇、粉锈宁、多菌灵、甲基硫菌灵、安克锰锌等5 种。研究结果证明,采用药剂拌种和选用抗病品种相结合的防治措施,可持续控制小麦秆黑粉病的流行为害。     

Bioactive 7-Oxabicyclic[6.3.0]lactam and 12-Membered Macrolides from a Gorgonian-Derived Cladosporium sp. Fungus

One new bicyclic lactam, cladosporilactam A (1), and six known 12-membered macrolides (2–7) were isolated from a gorgonian-derived Cladosporium sp. fungus collected from the South China Sea. Their complete structural assignments were elucidated by comprehensive spectroscopic investigation. Quantum chemistry calculations were used in support of the structural determination of 1. The absolute configuration of 1 was determined by calculation of its optical rotation. Cladosporilactam A (1) was the first example of 7-oxabicyclic[6.3.0]lactam obtained from a natural source. Compound 1 exhibited promising cytotoxic activity against cervical cancer HeLa cell line with an IC₅₀ value of 0.76 μM.     

Anti-Obese Effect of Glucosamine and Chitosan Oligosaccharide in High-Fat Diet-Induced Obese Rats

Objective: This study is to evaluate the anti-obese effects of glucosamine (GLC) and chitosan oligosaccharide (COS) on high-fat diet-induced obese rats. Methods: The rats were randomly divided into twelve groups: a normal diet group (NF), a high-fat diet group (HF), Orlistat group, GLC high-, middle-, and low-dose groups (GLC-H, GLC-M, GLC-L), COS1 (COS, number-average molecular weight ≤1000) high-, middle-, and low-dose groups (COS1-H, COS1-M, COS1-L), and COS2 (COS, number-average molecular weight ≤3000) high-, middle-, and low-dose groups (COS2-H, COS2-M, COS2-L). All groups received oral treatment by gavage once daily for a period of six weeks. Results: Rats fed with COS1 gained the least weight among all the groups (P < 0.01), and these rats lost more weight than those treated with Orlistat. In addition to the COS2-H and Orlistat groups, the serum total cholesterol (CHO) and low-density lipoprotein cholesterol (LDL-C) levels were significantly reduced in all treatment groups compared to the HF group (P < 0.01). The various doses of GLC, COS1 and COS2 reduced the expression levels of PPARγ and LXRα mRNA in the white adipose tissue. Conclusions: The results above demonstrated that GLC, COS1, and COS2 improved dyslipidemia and prevented body weight gains by inhibiting the adipocyte differentiation in obese rats induced by a high-fat diet. Thus, these agents may potentially be used to treat obesity.     

从品种角度试论提高中国烤烟质量的途径

烤烟质量与卷烟产品质量和烟农植烟效益密切相关,而品种是烤烟生产的基础,从品种角度探讨提高中国烤烟质量的途径和方法具有重要意义。笔者在国内学者研究文献的基础上,归纳了烤烟质量的评价标准及其影响因素,并结合中国烟叶生产实际和育种实践经验,分析了品种对烤烟质量的作用。认为对烤烟质量的评价要结合卷烟工业用户对烟叶质量的具体诉求,就某一特定的生态环境而言,品种是影响烤烟质量诸多因素中的最重要的因素,其对烟叶质量的影响包括遗传背景对烟叶质量的决定作用、与生态条件互作对烟叶质量的强化作用、抗逆抗病性对烟叶质量的保障作用、与生产技术协调对烟叶质量的提升作用诸方面。据此,提出了从品种角度提高中国烤烟质量的途径,包括：（1）面向烟叶生产实际,开展良种选育;（2）强化品种区域布局,实施适地适种;（3）针对品种特征特性,实现良种良法。     

安徽省油菜菌核病气象等级预报方法研究

为满足现代农业气象业务服务中病虫害发生发展气象等级预报需求,利用安徽省江淮、沿江和皖南3个区域代表站1991—2010年油菜菌核病观测数据和气象资料,通过相关分析,确定影响菌核病发生发展的主要影响时段和气象因子,再通过归一化处理、加权等方法得到影响油菜菌核病发生发展的综合气象条件指数。以综合气象条件指数为自变量,以油菜菌核病加权平均病株率为因变量,采用曲线回归方法,建立不同区域代表站油菜菌核病发生发展气象等级预报模型。模型拟合结果表明：江淮、沿江和皖南地区拟合准确率分别为80%、70%和75%;利用模型对2011—2013年发生情况进行预测,结果显示：江淮地区代表站全部正确,沿江和皖南地区代表站均为2年正确,一年与实际发生情况误差一个等级。建立的模型基本上能满足油菜菌核病气象等级预报业务服务的需求。     

加州鲈迟缓爱德华氏菌病的检测和防治

以江苏省吴江市八坼镇区域养殖场加州鲈鱼病鱼的细菌性病原体为研究对象。通过分离纯化,形态学观察,结合16S rRNA基因序列同源性检索,对该病原菌进行初步判断。同时进行药敏实验,了解该病原菌对各种抗生素的耐药性和敏感程度。研究结果:分离纯化得到2株纯菌株L2F和L2P2,革兰染色均呈红色,阴性杆状菌。16S rDNA序列分析,L2F和L2P2菌株与迟缓爱德华氏菌(Edwardsiella tarda)的同源性达99%。初步判断该菌为迟缓爱德华氏菌。药敏实验结果,该菌对磺胺异恶唑、链霉素和利福平等药物不敏感或耐药,对左氧氟沙星、诺氟沙星、恩诺沙星、强力霉素、氟苯尼考、头孢哌酮相对敏感。     

不同饵料对中华虎头蟹幼体发育和存活率的影响

为探讨中华虎头蟹苗种繁育过程中饵料及投喂的影响,进行了不同饵料对中华虎头蟹溞状幼体和大眼幼体生长发育和存活的影响实验。实验结果,中华虎头蟹各期幼体生长发育效果较好的饵料为动物性饵料,Z1期以卤虫无节幼体为开口饵料,只投喂扁藻后期幼体发育较差;Z2以轮虫和卤虫无节幼体的搭配为好;Z3期则以轮虫为适宜饵料;M期投喂卤虫无节幼体效果相对较好。     

Quantifying resistance to Plasmodiophora brassicae in Brassica hosts

Plasmodiophora brassicae causes clubroot of crucifers. A quantitative PCR (qPCR)‐based protocol was developed to measure P. brassicae DNA in the roots of susceptible, intermediately susceptible, intermediately resistant and resistant Brassica hosts, and the non‐host wheat, at 5, 10, 15, 20 and 42 days post‐inoculation (dpi). The final reaction of each plant genotype was recorded as an index of disease at 42 dpi. Plasmodiophora brassicae DNA showed an increase in susceptible and moderately resistant hosts from 5 to 42 dpi, in contrast to a decrease in a highly resistant host and the non‐host wheat over the same period. Index of disease was significantly positively correlated with the amount of P. brassicae DNA in the roots at 5, 15, 20 and 42 dpi in one experiment, and at 10, 15, 20 and 42 dpi in a repeated experiment. Significant positive correlations also existed between the amounts of P. brassicae DNA in the roots at 42 dpi and those at 5, 10, 15 and 20 dpi in one experiment, and those at 10, 15 and 20 dpi in a repeated experiment. The results generated by the qPCR assay were validated by microscopic examination of roots inoculated with P. brassicae. The qPCR‐based protocol developed in this study allows for the accurate quantification of P. brassicae DNA in host root tissues as early as 5 dpi, and may serve as a useful tool to evaluate pathogen proliferation and development in the roots.