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排序方式: 共有293条查询结果,搜索用时 15 毫秒
61.
Koglin A Mofid MR Löhr F Schäfer B Rogov VV Blum MM Mittag T Marahiel MA Bernhard F Dötsch V 《Science (New York, N.Y.)》2006,312(5771):273-276
Protein dynamics plays an important role in protein function. Many functionally important motions occur on the microsecond and low millisecond time scale and can be characterized by nuclear magnetic resonance relaxation experiments. We describe the different states of a peptidyl carrier protein (PCP) that play a crucial role in its function as a peptide shuttle in the nonribosomal peptide synthetases of the tyrocidine A system. Both apo-PCP (without the bound 4'-phosphopantetheine cofactor) and holo-PCP exist in two different stable conformations. We show that one of the apo conformations and one of the holo conformations are identical, whereas the two remaining conformations are only detectable by nuclear magnetic resonance spectroscopy in either the apo or holo form. We further demonstrate that this conformational diversity is an essential prerequisite for the directed movement of the 4'-PP cofactor and its interaction with externally acting proteins such as thioesterases and 4'-PP transferase. 相似文献
62.
Yano J Kern J Sauer K Latimer MJ Pushkar Y Biesiadka J Loll B Saenger W Messinger J Zouni A Yachandra VK 《Science (New York, N.Y.)》2006,314(5800):821-825
The oxidation of water to dioxygen is catalyzed within photosystem II (PSII) by a Mn(4)Ca cluster, the structure of which remains elusive. Polarized extended x-ray absorption fine structure (EXAFS) measurements on PSII single crystals constrain the Mn(4)Ca cluster geometry to a set of three similar high-resolution structures. Combining polarized EXAFS and x-ray diffraction data, the cluster was placed within PSII, taking into account the overall trend of the electron density of the metal site and the putative ligands. The structure of the cluster from the present study is unlike either the 3.0 or 3.5 angstrom-resolution x-ray structures or other previously proposed models. 相似文献
63.
Teeling H Fuchs BM Becher D Klockow C Gardebrecht A Bennke CM Kassabgy M Huang S Mann AJ Waldmann J Weber M Klindworth A Otto A Lange J Bernhardt J Reinsch C Hecker M Peplies J Bockelmann FD Callies U Gerdts G Wichels A Wiltshire KH Glöckner FO Schweder T Amann R 《Science (New York, N.Y.)》2012,336(6081):608-611
Phytoplankton blooms characterize temperate ocean margin zones in spring. We investigated the bacterioplankton response to a diatom bloom in the North Sea and observed a dynamic succession of populations at genus-level resolution. Taxonomically distinct expressions of carbohydrate-active enzymes (transporters; in particular, TonB-dependent transporters) and phosphate acquisition strategies were found, indicating that distinct populations of Bacteroidetes, Gammaproteobacteria, and Alphaproteobacteria are specialized for successive decomposition of algal-derived organic matter. Our results suggest that algal substrate availability provided a series of ecological niches in which specialized populations could bloom. This reveals how planktonic species, despite their seemingly homogeneous habitat, can evade extinction by direct competition. 相似文献
64.
The case of a 31-year-old captive female Western lowland gorilla (Gorilla gorilla gorilla) with decreased near vision but good distance vision is presented. Examination of the fundus revealed drusen-like bodies in the macula presumably because of an age-related macular degeneration (AMD). 相似文献
65.
Zini E Glaus TM Bussadori C Borgarelli M Santilli RA Tarducci A Margiocco ML Rampazzo A Meli ML Maisch B Pankuweit S 《Veterinary journal (London, England : 1997)》2009,179(2):225-229
Many viruses have been identified in pericardial fluid and in tissue samples from humans with pericarditis by means of molecular diagnostics. In canine idiopathic pericardial effusion there is as yet no conclusive evidence to support the involvement of an infectious agent. This study was designed to investigate a possible relationship between idiopathic pericardial effusion in dogs and viruses most commonly encountered in humans affected with viral pericarditis. Coxsackievirus B3 RNA, influenza virus type A RNA, human adenovirus type 2 DNA, human cytomegalovirus DNA, and parvovirus B19 DNA were investigated using PCR on pericardial effusion samples and pericardial tissue specimens collected from 14 dogs with idiopathic pericardial effusion. PCR was also used to test for two bacteria, Borrelia burgdorferi and Chlamydia pneumoniae. The same microorganisms were also looked for in pericardial effusions or pericardial washes from 10 dogs with neoplastic pericardial effusion, and in samples collected from 10 dogs which died of a non-cardiac disease. One pericardial effusion sample from a dog with the idiopathic form of the disease tested positive for influenza virus type A and sequencing of the amplicon confirmed the PCR result. In another dog from the same group a cytomegalovirus was detected by PCR in the effusion, but sequencing showed this to be a false-positive result. The genomes of the microorganisms investigated were not detected in neoplastic effusions or pericardial washes. The results indicate that viral and bacterial DNA/RNA of relevance for human pericarditis is rare in pericardial samples from dogs with idiopathic pericardial effusion. The finding of influenza type A viral RNA in pericardial fluid from one dog with the idiopathic form of the disease warrants further investigation. 相似文献
66.
67.
Anja Cehak Dr Med Vet Eckehard Deegen Prof Dr Dr Med Vet Wolfgang Drommer Prof Dr Dr Med Vet Elke Lütkefels Dr Med Vet Bernhard Ohnesorge PD Dr Med Vet Dipl ECEIM 《Journal of Equine Veterinary Science》2006,26(2):59-66
The purpose of this study was to establish a minimal invasive, transendoscopic technique for injection of poly-L-lactic acid into the free edge of the soft palate that can be performed on the standing sedated horse. Furthermore, whether the implant material induces an increase in fibrous tissue at the injection site, resulting in a greater rigidity of the soft palate, was determined.In 4 horses, the injection was performed bilaterally along the caudal margin of the soft palate, and the influence of the treatment on the soft palate function was determined. Clinical, radiographic and endoscopic examinations at rest were performed before and up to 168 days after injection according to a standardized examination protocol.In 6 horses, the implant material was injected unilaterally into the right half of the caudal margin of the soft palate. After clinical and endoscopic follow-up examinations, 2 horses each were killed 28, 84, and 168 days after the implantation procedure. At necropsy, macroscopic comparison of the treated and the untreated halves of the soft palate showed the influence on the size of the ostium intrapharyngeum and on the soft palate thickness. The localization of the implant material in the soft palate and the tissue response to the poly-L-lactic acid were evaluated histologically.Transendoscopic injection into the soft palate requires neither general anesthesia nor wound management, and it is noted for minimal tissue trauma. After injection, no alteration of the soft palate function was observed in horses with normal upper airway mechanics. One horse with intermittent dorsal displacement of the soft palate showed an improvement of the soft palate function for 112 days after implantation. No significant alteration in the size of the ostium intrapharyngeum was evident in any horse. Histologic assessment indicated the presence of implant material during the entire observation period of 168 days. The implant was accompanied by a granulomatous inflammation, and it was encapsulated by fibrous tissue. This may result in an increase in stiffness, and subsequent a greater rigidity of the soft palate, preventing it from displacement. The injection may therefore be considered an alternative to established therapies in horses showing dorsal displacement of the soft palate because of its unstable free edge. 相似文献
68.
Mirzakhayot Ibrakhimov Christopher Martius J.P.A. Lamers Bernhard Tischbein 《Agricultural Water Management》2011,101(1):52-61
Salinization of irrigated agricultural land threatens ecological sustainability and livelihoods of people. Salinization is especially severe in the dry lowlands world-wide and in Central Asia where large amounts of salts accumulated in the soil profile, originating from shallow saline groundwater (GW). Analysis of the unique dataset of 2000 monitoring wells of GW table and salinity in lowland Khorezm region of Uzbekistan over the period of 1990 till 2006 showed shallow GW levels of 1.1-1.4 m (±0.48-0.66 m) at start of leaching periods and 0.9-1.4 (±0.43-0.63 m) in July during the annual growing seasons. While leaching efficiency is decreased, shallow GW in July is far above the optimum levels of 1.4-1.5 m. The effects of topography, soil texture, and irrigation and drainage networks were found to favor shallow GW forced by excessive water diversion. The drainage network, which is seen by many specialists as underdeveloped and its improvement necessary to arrest unacceptable GW levels, is being used under its full capacity. The solution to alleviate land degradation is not only an improved drainage, but better controlled and more flexible water management. 相似文献
69.
70.
Tarduno JA Duncan RA Scholl DW Cottrell RD Steinberger B Thordarson T Kerr BC Neal CR Frey FA Torii M Carvallo C 《Science (New York, N.Y.)》2003,301(5636):1064-1069
The Hawaiian-Emperor hotspot track has a prominent bend, which has served as the basis for the theory that the Hawaiian hotspot, fixed in the deep mantle, traced a change in plate motion. However, paleomagnetic and radiometric age data from samples recovered by ocean drilling define an age-progressive paleolatitude history, indicating that the Emperor Seamount trend was principally formed by the rapid motion (over 40 millimeters per year) of the Hawaiian hotspot plume during Late Cretaceous to early-Tertiary times (81 to 47 million years ago). Evidence for motion of the Hawaiian plume affects models of mantle convection and plate tectonics, changing our understanding of terrestrial dynamics. 相似文献