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101.
6-BA和GA3促进三色堇再生的研究 总被引:4,自引:0,他引:4
为了解决三色堇生产周期长,观赏期短等问题,对三色堇进行了再生利用的研究。结果表明,对过期三色堇进行刈割,留茬再生,喷施6-BA和GA3,并加强肥水管理,能生产出具有良好商业价值的再生三色堇。其中喷施6-BA的花朵数多、花大、叶宽、株型适中,具有良好的观赏价值,观赏期延长1倍。 相似文献
102.
Irene Schiller Zen Huat Lu Lloyd Vaughan Roseline Weilenmann Stephane Koundrioukoff Andreas Pospischil 《Journal of veterinary diagnostic investigation》2003,15(6):585-588
Polymerase chain reaction (PCR) from paraffin-embedded tissues provides a powerful tool to amplify DNA from a variety of recent and archival material. Because DNA from paraffin-embedded samples is more degraded than from fresh material, the amplification of reference genes is essential to exclude false-negative results. This study describes the use of the proliferative cell nuclear antigen (PCNA) gene as a reference gene in a range of animal species and in humans. The PCNA-PCR to amplify a fragment extending from exon 5 through exon 6 and including the intervening intron 6 gave a reproducible pattern, with a 280-base pair (bp) band from canine, equine, bovine, ovine, and caprine samples showing high sequence homology. Porcine, guinea pig, tiger, and lion samples, however, gave an additional fragment of approximately 197 bp. The whole intron 6 from these fragments is missing, possibly representing a pseudogene. In feline samples only the 197-bp fragment could be detected. This study shows that the PCNA gene is highly conserved across a broad range of animal species and is well suited as an internal control for PCR analysis in veterinary medicine. 相似文献
103.
Sabine Riesen DR MED VET Valerie Schmid DR MED VET Lorrie Gaschen DVM DR MED VET Andre Busato DR MED VET MSC Johann Lang PD DR MED VET 《Veterinary radiology & ultrasound》2002,43(6):554-560
The purpose of this study was to measure splanchnic blood flow during digestion in unsedated dogs by using duplex Doppler sonography. The study population consisted of 12 healthy dogs. Blood flow in the cranial mesenteric artery, the celiac artery, and the aorta was measured before a test meal and at 20, 60, and 90 minutes after eating. The following measurements were made or calculated: vessel diameter, peak systolic velocity, end diastolic velocity, mean velocity, resistive index, pulsatility index, and flow volume. There was a significant postprandial decrease in the resistive and pulsatility indices in both the cranial mesenteric (preprandial RI = 0.867, postprandial RI = 0.796, preprandial PI = 3.033, postprandial PI = 2.173) and the celiac (preprandial RI = 0.854, postprandial RI = 0.769, preprandial PI = 2.639, postprandial PI = 1.930) arteries. In both vessels the end diastolic velocity, the mean velocity, and the flow volume increased significantly postprandially. These changes occurred significantly earlier in the celiac artery than in the cranial mesenteric artery. The findings most likely correspond to postprandial splanchnic vasodilation. Doppler ultrasound provide a good methode of detecting changes in postprandial splanchnic blood flow in the dog. 相似文献
104.
对冬枣采后主要病害种类、症状及防治方法进行阐述,并介绍了生物防治技术在果蔬贮运病害防治中的应用,展望了该技术在冬枣采后病害防治中的应用前景. 相似文献
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108.
Devesa V Loos A Súñer MA Vélez D Feria A Martínez A Montoro R Sanz Y 《Journal of agricultural and food chemistry》2005,53(26):10297-10305
A study of the transformation of arsenic species by the microflora of the freshwater crayfish Procambarus clarkii was carried out. The study of the degradation of AB (arsenobetaine) was performed in aerobic conditions in two culture media (tryptic soy broth and saline medium) at two temperatures (30 and 8 degrees C). The microflora transformed AB into TMAO (trimethylarsine oxide), DMA (dimethylarsinate), MA (methylarsonate), and an unidentified compound (U1). The quickest transformations were carried out by microflora from hepatopancreas incubated in saline medium at 30 degrees C. The individualized study of other arsenic species [AC (arsenocholine), TETRA (tetramethylarsonium ion), TMAO, DMA, and MA] was also performed in saline medium. The only transformation observed was of AC into AB. The bacteria possibly responsible for AB degradation were isolated, identified by phenotypic and genotypic methods, and individually assayed for AB transformation. Only isolates allocated to the species Pseudomonas putida were able to metabolize AB. 相似文献
109.
Lars A. Knechtenhofer Irene O. Xifra Andreas C. Scheinost Hannes Flühler Ruben Kretzschmar 《植物养料与土壤学杂志》2003,166(1):84-92
To assess the mobility of Pb and associated metals in a highly contaminated shooting range soil (Losone, Ticino, Switzerland), we investigated the spatial distribution of the metals and their relation to preferential water flow paths. A 2.2 m2 plot located 40 m behind the stop butt was irrigated with a solution containing bromide and Brilliant Blue, a slightly sorbing dye. A soil profile 50 cm in width was sampled down to 80 cm with a spatial resolution of 2.5 cm, resulting in 626 samples. Concentrations of elements (12 ≤ Z ≤ 92) were determined by energy‐dispersive Xray fluorescence spectrometry, and Brilliant Blue concentrations were determined with a chromameter. In the acidic (pH 3), organic matter‐rich, well drained Dystric Cambisol, maximum concentrations of 80.9 g kg‐1 Pb, 4.0 g kg‐1 Sb, and 0.55 g kg‐1 Cu were measured in the topsoil. Within 40 cm soil depth, however, Pb, Sb, and Cu approached background concentrations of 23 mg kg‐1, 0.4 mg kg‐1, and 9.4 mg kg‐1, respectively. The even horizontal distribution and the steep gradient along soil depth indicate tight metal binding in the topsoil, and a fairly homogeneous transport front. In contrast, water flow through the profile was highly heterogeneous. In the uppermost 20 cm, preferential flow was initiated by heterogeneous infiltration at the soil surface, but had no influence on metal distribution. Below 20 cm, however, preferential flow originated from larger tree roots, and metal concentrations were significantly elevated along these macropores. Spatial distributions of Pb, Sb, and Cu were similar, suggesting that all three metals are strongly retained in the topsoil and transported along preferential water flow paths in the subsoil. 相似文献
110.
Andreas B. Wolff Maria Sidirelli Constantinos Paradellis Kiriakos Kotzabasis 《Biology and Fertility of Soils》1995,20(4):249-252
The relationship between soil acidity and polyamine (putrescine, spermidine, spermine) concentrations in roots was evaluated and compared to (1) nodule numbers in common beans and (2) tannin concentrations in roots. Six Phaseolus vulgaris cultivars were grown in pots in a greenhouse in soil at pH 4.5 or 5.5 and inoculated with Rhizobium leguminosarum bv. phaseoli strains. At pH 4.5 nodule numbers were strongly reduced but polyamine concentrations were 50% higher than at pH 5.5. At both pH levels putrescine and spermidine were the dominant polyamines, while the concentration of spermine was less than half of the other two. There was also a cultivar effect on the polyamine concentration, but this was much less pronounced than the pH effect. By 22 days after planting the uninoculated control plants had about 13% lower levels of polyamines than the inoculated plants. The concentrations of putrescine, spermidine, and spermine were negatively correlated with nodule numbers, but positively correlated with the root tannin content. There were no significant correlations with either root or plant dry weight. 相似文献