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71.
72.
Polar solvents often exert a dramatic influence on reactions in solution. Equilibrium aspects of this influence involve differential solvation of reactants compared to the transition state that lead to alteration of the free-energy barrier to reaction. Such effects are well known, and often give rise changes in reaction rates of many orders of magnitude. Less well understood are effects arising from non-equilibrium, dynamical aspects of solvation. During the course of reaction, charge is rapidly redistributed among reactants. How the reaction couples to its solvent environment depends critically on how fast the solvent can respond to these changes in reactant charge distribution. In this article the dynamics of solvation in polar liquids and the influence of this dynamics on electron-transfer reactions are discussed. A molecular picture suggests that polar solvation occurs on multiple time scales as a result of the involvement of different types of solvent motion. A hierarchy of models from a homogeneous continuum model to one incorporating molecular aspects of solvation, combined with computer simulations, gives insight into the underlying dynamics. Experimental measures of solvation dynamics from picosecond and subpicosecond time-dependent Stokes shift studies are compared with the predictions of theoretical models. The implication of these results for electron-transfer reactions in solution are then briefly considered. 相似文献
73.
74.
Fitzpatrick DF Fleming RC Bing B Maggi DA O'Malley RM 《Journal of agricultural and food chemistry》2000,48(12):6384-6390
Previous work has shown that red wines, grape juices, and other grape products cause endothelium-dependent relaxation (EDR) of blood vessels in vitro by increasing nitric oxide production. In this paper we describe the isolation and characterization of some of the compounds responsible for EDR activity. Concord grape seeds were extracted with methanol and the compounds were separated by Toyopearl TSK HW-40S chromatography. Resulting fractions (primarily phenolic acids, catechins, and proanthocyanidins) were further separated semipreparatively by reversed-phase HPLC, and peaks were collected and bioassayed for EDR activity using the rat aorta preparation. EDR-active compounds were subsequently characterized by HPLC retention times and electrospray-ion-trap mass spectrometry. The compounds exhibiting the most EDR activity were proanthocyanidin trimers, tetramers, pentamers, and polymers and their gallates, as well as a dimer gallate (EC50 values in the range of 0.6-2.5 microg catechin equivalents/mL). These compounds should be useful for in vitro and in vivo studies, particularly as they relate to improvement of cardiovascular function. 相似文献
75.
Summary Seeds of the long-time maize (Zea mays L.) inbred CI 21 (Athens) were prepared with the following cytoplasms: GA 199 and GT 112. Each source of cytoplasm as the female parent was corssed to the CI 21 (A) as the pollen parent. The F1, as the female, was backcrossed successively to the CI 21 (A) genotype until the CI 21 (A) nucleus was substituted into each of the cytoplasms. Thus, each cytoplasmic source of seed assumed to have the same nuclear genotype.The cytoplasms were compared in all possible combinations for one year with 10 replications. Among the characters studied, significant differences were obtained for the following: effect of herbicide, reaction to Fusarium sp., silking on a given day, tasseling, plant height, ear height, lodging, number of ears, and yield.These results revealed a favorable interaction of GA 199 cytoplasm with the CI 21 (A) genotype for desirable characters. Apparently, plasmon-sensitive effects caused the unfavorable results. Results from these experiments also provide further support for cytoplasmic effects on agronomic characters in maize.Part of the dissertation submitted by the senior author to the Dep. of Agronomy, University of Georgia, Athens, Georgia 30602, USA. 相似文献
76.
S. Cooper-Bland M. J. De Maine H. E. Stewart M. L. M. H. Fleming M. S. Phillips A. Kumar 《Euphytica》1996,90(2):209-216
Intraspecific tetraploid somatic and sexual hybrid plants have been resynthesised following protoplast fusion and by sexual crosses between two dihaploid potato (Solanum tuberosum) lines each possessing complementary agronomic traits. The dihaploid PDH 40 possesses good tuber shape and yield but has foliage susceptibility to late blight (Phytophthora infestans). On the other hand, the dihaploid PDH 727 possesses resistance to blight in the foliage but has a low yield of small and irregular shaped tubers. Since it was only possible to use a partial selection strategy based on culture media to facilitate recovery of somatic hybrid plants-further morphological and esterase isozyme based characterisations were performed to identify somatic hybrid plants from amongst the non-hybrid plant material. When the blight resistance of both the intraspecific somatic and sexual hybrid plants was assessed there was no significant difference in the mean resistance value and it was intermediate between those of their parents. However, the range of resistance was much wider among the sexual hybrids than among the plants derived from somatic fusion. An assessment of tuber yield between tetraploid sexual and somatic hybrids showed no significant difference and it was higher than that of either parent value. The implication of these results in the context of potato genetics and breeding is discussed. 相似文献
77.
Kaewmongkol G Kaewmongkol S Burmej H Bennett MD Fleming PA Adams PJ Wayne AF Ryan U Irwin PJ Fenwick SG 《Comparative immunology, microbiology and infectious diseases》2011,34(5):411-417
A variety of Bartonella species were detected in two species of ticks and three species of fleas collected from marsupial hosts; brush-tailed bettong or woylie (Bettongia penicillata) and western barred bandicoots (Perameles bougainville) and from a rodent host; Rattus fuscipes in Western Australia. Bartonella species were detected using nested-PCR of the gltA gene and the 16S–23S ribosomal internal transcribed spacer region (ITS), and species were characterized using DNA sequencing of the 16S rRNA, gltA, rpoB, ftsZ genes and the ITS region. Bartonella rattaustraliani and B. coopersplainsensis were detected in Ixodes spp. ticks and fleas (Stephanocircus pectinipes) respectively collected from rodents. Two novel Bartonella species were detected from marsupials; Candidatus Bartonella woyliei n. sp. was detected in both fleas (Pygiopsylla hilli) and ticks (Ixodes australiensis) collected from woylies and Candidatus Bartonella bandicootii n. sp. was detected in fleas (Pygiopsylla tunneyi) collected from western barred bandicoots. Concatenated phylogenetic analysis of all 5 loci clarified the marsupial cluster of Bartonella species in Australia and confirmed the species status of these two Bartonella species in ticks and fleas from woylies and western barred bandicoots, which are classified as threatened species and are vulnerable to extinction. 相似文献
78.
Miller M Terrell S Lyashchenko K Greenwald R Harris B Thomsen BV Fontenot D Stetter M Neiffer D Fleming G 《Journal of zoo and wildlife medicine》2011,42(3):468-472
Two adult female bontebok (Damaliscus pygarus dorcas) were euthanized because of signs of pneumonia and weakness (case 1), and a nonresponsive lameness with draining fistula (case 2). Necropsy findings were similar in both cases and consisted of disseminated granulomatous lesions in the liver, kidneys, spleen, lungs, pleural surfaces, and multiple lymph nodes. Mycobacterium kansasii was isolated from both cases after multiple attempts on a variety of samples by two laboratories. The remaining four animals in the herd were tested for antibody responses using the Chembio ElephantTB STAT-PAK, DPP VetTB kits, and multi-antigen print immunoassay (MAPIA), for immune reaction using the intradermal tuberculin test, and by tracheal wash cultures, and thoracic radiographs. Banked serum samples collected in 2005 and obtained from the original institution, revealed 1/9 (11.11%) seropositive animals using the three immunoassays. Retesting the current herd in 2008 showed 2/6 (33.33%) seropositive animals by the three tests, with MAPIA demonstrating antibody reactivity to MPB83 and MPB70 proteins. Inconsistent intradermal tuberculin test results, cross-reactivity in serologic assays designed for tuberculosis detection, difficulty in obtaining definitive identification by culture, and inability to identify a source of infection created challenges in distinguishing the atypical mycobacteriosis due to M. kansasii from the initially suspected tuberculous infection in this herd. Owing to regulatory considerations, differences in host-to-host transmission, and source of infection between Mycobacterium tuberculosis complex and nontuberculous mycobacteria, correct diagnosis is crucial for management of these diseases in wildlife species. 相似文献
79.
Fleming JM Platt SR Kent M Freeman AC Schatzberg SJ 《Journal of Feline Medicine and Surgery》2011,13(12):992-996
A 6-month-old female spayed domestic shorthair cat was presented for evaluation of a focal subcutaneous swelling on the dorsal neck at the level of atlas. The magnetic resonance imaging and surgical treatment of a dermoid sinus associated with the cervical vertebrae is described. To the authors' knowledge, a dermoid sinus in this location has not been described previously in the cat. The prognosis following surgical resection appears favorable. 相似文献
80.
S A Fleming E L Hunt C Brownie A Rakes B McDaniel 《American journal of veterinary research》1992,53(2):222-224
Samples of serum and urine were obtained simultaneously from 56 healthy lactating cows to determine ranges of fractional excretion (FE) of calcium (Ca), phosphate (PO4), magnesium (Mg), sodium (Na), potassium (K), and chloride (Cl). Samples were obtained at 3 stages of lactation: period 1 = 1 to 7 days, 2 = 83 to 112 days, and 3 = 175 to 197 days. The FE of electrolytes were significantly different among periods 1, 2, and 3 for Ca (P less than 0.001), PO4 (P less than 0.025) and Mg (P less than 0.025), but were not significantly different for Na, K, and Cl. Least squares mean FE of Ca was lowest in period 1 and not significantly different for periods 2 and 3, whereas mean FE values for PO4 and Mg were highest in period 2 and not significantly different for periods 1 and 3. The mean FE values of Na, K, and Cl did not change with stage of lactation. Age and category of milk production (high, medium, and low) did not influence the FE values of the electrolytes. 相似文献