Effect of supernatants from cord blood mononuclear cells and peripheral blood mononuclear cells on infection of HIV-1 in vitro

AIM: To study the effect of supernatants from cord blood mononuclear cells (CBMC) and peripheral blood mononuclear cells (PBMC) on infection of HIV-1 in vitro, and develop effectively soluble factors for human acquired immunodeficiency syndrome (AIDS) treatment. METHODS: Different supernatants from CBMC and PBMC activated by PHA for 5 hours and 12 hours were added to cell culture systems between HIV-1ⅢB/H9 cells labeled by calcein-AM and MT-2 cells, then to count the fusion under a reverted fluorescent microscope after 2 hours, respectively, and different soluble factors in supernatants were detected by Luminex 100TM. RESULTS: These supernatants from CBMC and PBMC activated by PHA for 5 hours and 12 hours inhibited the formation of fusion, and there is no difference between supernatants collected in CBMC and PBMC at same time point. However, the supernatants collected in 5 hours were more effective to inhibit the formation of fusion than those in 12 hours (P<0.05). There was a lower concentration for pro-inflammatory cytokines, such as IFN-γ, MCP-1 and TNF-α, in supernatants from CBMC than those from PBMC, but the concentration of MIP-1a and RANTES in supernatants from CBMC was higher than those from PBMC. CONCLUSION: The soluble factors for anti-HIV can be prepared from CBMC and PBMC, which is a new source of anti-HIV therapeutic preparation.     

《科技导报》（半月刊）欢迎订阅、欢迎投稿、欢迎刊登广告

AIM: To study the effects of ultraviolet (UV) on mitochondrial functions and apoptosis in HaCaT cells. METHODS: After irradiation by UV at low dose (UVA 2 J/cm2，UVB 10 mJ/cm2) and high dose (UVA 6 J/cm2，UVB 30 mJ/cm2), HaCaT cells were cultured for 15 hours. Flow cytometry was used to measure mitochondrial membrane potential, mitochondrial mass and apoptotic rate. Annexin V-FITC/PI staining of apoptotic cells was analyzed by laser confocal microscopy. RESULTS: After UV irradiation, cell proportion with low mitochondrial membrane potential increased with irradiation doses. The proportion of control group, low dose group and high dose group were 7.94%±1.02%, 25.87%±4.55% and 39.27%±5.32%, respectively. Cells proportion with low mitochondrial mass increased with irradiation doses. The proportion of control group, low dose group and high dose group were 15.19%±1.58%, 40.36%±4.41% and 68.79%±5.46%, respectively. The hypodiploid peaks of DNA content analysis represented the apoptotic rate of HaCaT cells. The apoptotic rate of control group, low dose group and high dose group were 1.82%±0.51%, 30.16%±5.47% and 58.49%±5.98%, respectively. To analyze the cells apoptosis by staining with annexin V-FITC and PI, the results were consistent with those of DNA content analysis. Cells in control group showed almost no positive staining cells. Single annexin V-FITC positive cells in low dose group and double positive cells in high dose group were predominant, respectively. CONCLUSION: UV irradiation induces HaCaT cell mitochondrial depolarization, as well as mitochondrial mass loss. These changes are related to cell apoptosis.     

VEGF induces HUVECs to produce extracellular H2O2 and its proliferation role

AIM:To study the effect of VEGF on extracellular H₂O₂ production in HUVECs and the role of H₂O₂ in the VEGF-induced proliferation. METHODS:HUVECs was stimulated with 500 μg/L VEGF. Products of extracellular H₂O₂ was detected by H₂DCFDA staining. MTT method was used to value the influences of 3×10⁶ U/L catalase and 5-20 mmol/L H₂O₂ to VEGF function. RESULTS:After treatment for 15 min with VEGF, HUVECs appeared fluorescence, and continued to become stronger, peaked at 45 min then decreased. HUVECs, which was treated simultaneity with VEGF and 3×10⁶ U/L catalase, only appeared very faint fluorescence. The proliferation of HUVECs by VEGF was restrained when treated with 3×10⁶ U/L catalase. The extrinsic H₂O₂ at concentration of 5-10 mmol/L promoted the proliferation of HUVECs but inhibited the proliferation effect of VEGF on HUVECs (P<0.01). CONCLUSION:These findings indicate that VEGF induces HUVECs to produce extracellular H₂O₂ and plays role in proliferation, but extrinsic H₂O₂ restrains VEGF function.     

银杏种子UDPG焦磷酸化酶特性的研究

银杏(Ginkgo biloba L.)种子中UDPG PPlase最适温度为29-30℃,最适pH为7.5-80,二价阳离子中Zn~(2 )、Ba~(2 )对其活性影响较小.Mn~(2 )、Mg~(2 )、Ca~(2 )对其有明显影响,而Cu~(2 )、Pb~(2 )有强烈的抑制作用.银杏种子幼小至膨大期,UDPG PPlase活性较低;种子成熟期至贮藏初期,活性迅速而显著地增强;贮藏过程中活性保持较高水平.     

淡水环境中可溶有机质研究进展

论述了淡水环境中可溶有机质(DOM)在分离与分析方法、来源与特征,以及环境意义等方面的研究进展。指出了在分离方法上,从XAD树脂分离到反渗透膜分离已经发展出各种分离技术;在分析方法上,现代波谱分析技术得到了广泛应用。DOM是由在来源、年龄、存在状态、后生改造等方面存在巨大差异的不同有机质混合构成的,因而呈现出十分复杂的非均质结构。DOM通过其自身的生物可利用性、改变有机污染物和重金属的生物可利用性及其耗氧能力影响环境质量,而且其自身的一些地球化学特征也能用来反映环境的变化特点。最后简述了湿地淡水环境中DOM的研究趋势和展望。     

痣鳞鳃金龟的生物学特性与防治

痣鳞鳃金龟二年发生一代 ,此虫在砂质土壤的林地密度大 ,为害严重。造林时在每株根际施 5g 3 %呋喃丹颗粒剂 ,可有效地控制此虫的危害并兼治其它地下害虫和造林初期的食叶害虫。     

基于LabWindows/CVI的风洞数据检测系统

以虚拟仪器软件开发环境LabWindows/CVI为开发平台,开发了用于风洞风速数据检测与分析的虚拟仪器系统,以利用可移动式风蚀风洞开展土壤风蚀的研究.系统具有数据采集、运算、分析、显示和存储等功能.经实验验证,其性能稳定可靠,操作方便,提高了风蚀风洞内风速和风廓特性检测分析的精度及自动化程度.     

黄糊精/秸秆纤维复合材料弯曲性能分

利用模压方法制备了黄糊精/秸秆纤维复合材料,并利用试验方法研究黄糊精/秸秆纤维复合材料的弯曲性能.研究结果表明:在试验条件下,通过对黄糊精/秸秆纤维复合材料弯曲强度和弯曲弹性模量的极差分析发现最优组合相同,即热压温度A=170℃、热压压力B=9.8 MPa、热压时间C=20 min、黄糊精质量D=30 g,并对最优组合进行了试验验证,得出其弯曲强度为15.34 MPa,弯曲弹性模量为2 522.78 MPa.通过对复合材料的弯曲强度和弯曲弹性模量的方差分析发现,热压时间是不显著因素,而热压压力是显著因素.     

江苏省流域生态管理的若干问题研究

在分析江苏省流域管理现状及存在的问题基础上,对实施流域生态管理过程中涉及的若干问题研究,提出:①完善流域生态管理的政策支撑体系,要有相关法律法规制度保障、有完善的规划体系可执行;②改革流域管理体制,打破流域与行政区域的隔阂,建立江苏省流域委员会;③创新流域生态管理机制,建立流域安全机制、生态补偿机制、流域协作轴线、多参与流域决策系统和虚拟水战略等;④强化流域生态管理的技术支持,施行生态管理技术和数字流域建设等。     

基于Fluent的风洞典型风蚀地表风速廓线的模拟

利用计算流体动力学(CFD)软件Fluent对OFDY-1.2型可移动式风蚀风洞中模拟典型农田风蚀地表风速廓线进行数值模拟,通过改变棒栅、尖塔和粗糙元的尺寸、形状和位置,分别用软件和室内风洞实验模拟出了与野外实地测试廓线相吻合的风速廓线。同时,对野外实测、室内风洞模拟和软件数值模拟的风速廓线进行了离散程度分析。