内窝孔式棉花精量穴播轮的试验

目前,新疆棉花种植广泛采用半精量穴播轮,其原理是勺式取种,存在下种量大、重播率高、下种不均匀、后续工作量大等缺陷,已不适应当前棉花产业发展要求。气吸式精量穴播轮可实现一穴一粒,准确率高,但气吸式棉花穴播机结构复杂、造价高,对机具维护、使用者技能以及整地有较高要求。另外,作业时拖拉机须保持高速运转,能源消耗大,因而限制了气吸式精量穴播轮的普及和推广。为此,将内窝孔取种原理应用于棉花精量取种,介绍了内窝孔式穴播轮的结构和工作过程,并进行了排种试验和分析。由试验得知,内窝孔式取种原理可用于新疆棉花的精量取种。     

基于模糊层次分析法的农产品加工企业风险评价研究

风险评价是风险管理的重要阶段,针对农产品加工企业特点,提出了一种基于模糊层次分析法的农产品加工企业风险评价方法。该方法初步解决了农产品加工企业风险管理中的关键问题——风险因素的识别。以某农产品加工公司为例,介绍了该方法的应用过程。     

大型悬吊井筒抽芯式泵机组安装质量控制

由于悬吊井筒抽芯式泵机组结构不同于传统的苏式泵，按原有的规范和要求不能保证该型机组安装检修质量．仅以厂家提供的说明书要求进行安装，因制造工艺、精度等原因，运行过程中，机组稳定性差，故障率高等一直困扰着使用单位．为保证机组安装质量，消除机组运行中常见的不正常现象和故障，以64LKXA-24．5型立式泵机组为例，针对该型机组结构形式的特殊性，根据规范的安装要求，结合安装检修过程中存在的问题，介绍了该形式泵机组的主要安装方法，提出了相应的注意事项．     

奉化市“十二五”期间年森林采伐限额的编制

根据编制原则及范围,采用2007年森林资源二类调查成果,利用国家林业局调查规划设计院开发的"森林合理年采伐量测算系统"和由浙江省森林资源监测中心开发的"浙江省十二五编限数据处理系统"组成的软件,测算出奉化市合理年采伐量为49413m3.为利于林业可持续发展,确定奉化市"十二五"期间年森林采伐限额建议指标为46980m3,其中主伐4440 m3,抚育采伐32970 m3,更新采伐5570 m3,其它采伐4000 m3.     

新农村建设筹资中的问题与对策

新农村建设是一项复杂的系统性工程,有无充足的资金支持决定了新农村建设的成败,该文分析了现有的主要筹资渠道的问题,提出了如何完善现有筹资渠道,广开源头,为新农村建设提供不竭的动力支持的建议。     

Fungicidal activity of compounds extracted from the pericarp of Areca catechu against Colletotrichum gloeosporioides in vitro and in mango fruit

Areca catechu L., a member of the Palmaceae family, is one of the most commonly used drugs in the world. Compounds obtained from the hexane, ethyl acetate and methanol extracts of the pericarp of A. catechu L. were assessed in vitro and in mango fruit for antifungal activity against Colletotrichum gloeosporioides Penz. In vitro studies also indicated that three triterpenes, namely fernenol (fern-9(11)-en-3α-ol), arundoin (fern-9(11)-en-3α-ol ME), and a mixture of stigmasterol and β-sitosterol, and one fatty acid, lauric acid, could inhibit the mycelial growth of C. gloeosporioides with EC₅₀ values of 36.7, 47.5, 56.7 and 111.5 mg L^?1, respectively. Furthermore, fernenol, arundoin, and the mixture of stigmasterol and β-sitosterol highly inhibited spore germination and germtube elongation. Mango fruit studies suggested that fernenol, arundoin and the mixture of stigmasterol and β-sitosterol were significantly more effective than benomyl for controlling postharvest anthracnose disease when used at 100 and 200 mg L^?1.     

Comparison and analysis of the staining methods for cholesterol

Cholesterol staining is a useful approach for the visualization, localization and quantification of cholesterol in cells or tissues, which is frequently used to investigate the mechanisms of some diseases such as arteriosclerosis, Niemann-Pick disease type C, and Alzheimer's disease. It can be accomplished through various microscopes including light microscope, fluorescent microscope, and electronic microscope. During the past decades, various types of methods for cholesterol staining with different principles have been established for different applications. It is important to choose an appropriate method that is suitable for particular experimental aims, features and conditions. At present, three kinds of methods are frequently applied: filipin fluorescent method, BCθ(a biotinylated and carlsberg protease-nicked derivative of perfringolysin O) toxin method, and cholesterol oxidase-diaminobenzidine(oxidase-DAB) method. Four kinds of methods are scarcely applied: Schultze method, perchloric acid-naphthoquinone method(PAN), digitonin method, and o-phthalaldehyde method. In this review, the principles, advantages, and disadvantages of these methods are compared with the emphasis of the application, sensitivity, and specificity.     

丝绸之路南道旅游资源开发研究-以和田地区为例

丝绸之路南道不论在自然旅游资源,还是在人文旅游资源方面都有其独特之处。本文通过对和田地区旅游资源和客源市场现状分析,提出围绕丝绸之路文化旅游为核心,突出以玉石故乡,大漠风光,田园绿洲,佛国古城及伊期兰教四大特色为主题,重点推出“和田玉石文化”、“尼雅寻梦”等精品,以此为龙头,带动区域旅游业发展的旅游资源开发策略。并提出“一主线,六干线”的各具特色的旅游线路。     

关于随机小修费用模型的最优维修策略

采用变分方法考察了具有随机小费用的可修模型的维修策略问题，推导出了依赖年龄的最倨费用界维修策略所满足的微分方程及其边界条件。从而得到了费用界策略形式下的最优。对于包含预防性更换的可修模型也同样得到了费用界－预更换策略形式下的最优解。     

Structure and Expression In planta of Botrytis cinerea Ubiquitin Genes

To identify genes of the necrotrophic pathogenic fungus Botrytis cinerea that are expressed during infection of tomato leaves, a differential screening of a genomic library with radioactively labelled cDNA was performed. This resulted in the identification of a B. cinerea gene, denominated Bcubi4, which encodes a precursor protein consisting of four identical head-to-tail repeats of a 76aa ubiquitin unit. Subsequently a gene denominated Bcubi1CEP79, encoding a single ubiquitin unit joined to a Carboxyl Extension Protein of 79 amino acids, was isolated. The expression of the two ubiquitin genes was studied during pathogenesis of B. cinerea on tomato. Bcubi1CEP79, but not Bcubi4, mRNA was transiently induced at 16h after inoculation. The increased expression of the Bcubi1CEP79 gene at this stage of pathogenesis might be required for enhanced ribosomal biogenesis.