用于冬小麦组分信息检测的便携式LVF光谱仪研究

小麦是我国主要的粮食作物,能够实时准确地对小麦进行长势监测具有重要意义。为此,介绍了一种自主研发的便携式LVF光谱仪的软硬件结构,采集冬小麦冠层叶片光谱数据,利用偏最小二乘法建立了冬小麦组分信息的内嵌反演模型,使其成为可以测量冬小麦叶片组分信息的专用型光谱仪。同时,用校验集对预测模型进行验证,相关系数分别达到0.906和0.876。结果表明:便携式LVF光谱仪在冬小麦叶片组分信息的检测方面是可行性的。     

Establishment and Application of a Multiplex RT-PCR Assay for Detection of PEDV,TGEV and PRoV

In this study,a multiplex RT-PCR assay was established to differentially detect porcine epidemic diarrhea virus (PEDV),porcine transmissible gastroenteritis virus (TGEV) and porcine rotavirus (PRoV) after optimization of the reaction conditions.Three pairs of primers PEDV-N,TGEV-M and PRoV-VP6 were designed for specifically amplifying PEDV N gene,TGEV M gene and PRoV VP6 gene,respectively.The assay could specifically amplify PEDV,TGEV and PRoV,but not classical swine fever virus (CSFV),porcine foot and mouth disease virus (FMDV),pseudorabies virus (PRV),porcine parvovirus (PPV) and porcine circovirus type 2 (PCV2).The detection limits of PEDV,TGEV and PRoV standard recombinant plasmids were 1.41×10³,1.41×10² and 1.41×10³ copies/μL,respectively.The repeated reaction under the same conditions obtained uniform results.The assay was used to detect a total number of 190 clinical samples,of which 42 (22.11%) samples were positive for PEDV,58 (30.53%) samples for TGEV and 34 (17.89%) samples for PRoV,and there were mixed infection among these viruses.The results indicated that this multiplex RT-PCR assay had the advantages of sensitivity,specificity and repeatability and provided a useful tool for differential detection and epidemiological investigation of PEDV,TGEV and PRoV.     

Effects of Supplemental Complex Water Soluble Vitamin on Production Performance,Blood Biochemical Parameters and Oxidation Resistance of Dairy Cows

The objective of the study was to investigate the effects of supplemental complex water soluble vitamins on production performance, blood biochemical parameters and oxidative resistance of dairy cows in spring and summer.The experiment was designed by 2×2 factorial randomized blocks design.Eighty healthy dairy cows with similar parity and body weight were randomly divided to four groups, with 7 days of adaptation and 70 d experimental phase.The dairy cows in group Ⅲ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in spring.The dairy cows in group Ⅱ and Ⅳ were fed a basal diet supplemented with complex water soluble vitamins in summer.The results showed that the ADFI and daily milk yield of dairy cows receiving water soluble vitamin in spring were extremely significantly higher (P<0.01);The ADFI of dairy cows receiving water soluble vitamin was significantly higher in summer (P<0.05) and daily milk yield were extremely significantly higher (P<0.01);The butterfat rate was extremely significantly increased and SCC was extremely significantly decreased in spring and summer (P<0.01);Supplementation of water soluble vitamin was also significantly increased LYM (P<0.05).In summer, T-AOC, T-SOD were extremely significantly increased (P<0.01), and TP, CK, LDH, TP, HDL and ALB were extremely significantly decreased (P<0.01) by water soluble vitamin supplementation than that of control group.In conclusion, complex water soluble vitamins could increase the production performance and non-specific immune function, improve the oxidative resistance and relieved the heat stress of the dairy cows.     

发展农业机械化,建设社会主义新农村

现代化的农机装备作为先进的劳动工具,推动了生产力和文明社会的快速发展。建设社会主义新农村,农业机械化既是充要条件,又是必要条件;农业机械化是实现生产发展、生活宽裕、村容整洁、环境和谐的手段、条件、保证。没有农业的机械化,不可能有真正意义上的社会主义新农村建设。     

低能离子注入麻黄种子生物效应初步研究

本文初步研究了离子束注入麻黄种子后的当代生物效应 ,研究表明 :离子注入对麻黄种子的生长起良好的生物效应。注入剂量为 4× 1 0 1 6 N+ / cm2 时 ,伊吾蓝麻黄种子发芽率最高为 76 % ,相对空白增长 4 3.4 % ;在注入剂量为 4× 1 0 1 6 N+ / cm2 ,伊吾蓝麻黄种子的发芽 3天时呼吸速率达 1 .1 4 0 mg/ g· h。这也表明发芽率高的剂量下 ,种子的呼吸速率增加 ,注入剂量为 1 2× 1 0 1 6 N+ / cm2 ,伊吾蓝麻黄种子的盆栽出土率最大为 2 2 .7%。 4× 1 0 1 6 N+ / cm2 剂量离子注入和硕蓝麻黄种子时 ,其盆栽出土率最大为 4 2 .3% ,比对照增加了 6 4 %。     

ON STUDY DIGGED FIELD MOVEMENT''S LAW BY EXPERIMENTAL METHOD OF LASER HOLOGRAPHIC MOIRE INTERFERMETRY

The high-precision displacement measuring technology of optic-measuring mechanics is, for the first time,successfully applied on the similar model of the model land-layer with a new-type variable modulus of elasticity. The angle of movement and the distributed displacement of upper and lower rock-layers are measured. The technology is a combination of laser's technology and the modern chemical sticker-it is called the sticking moire interferometry. Compared with the measured values, the result is very satisfactory.     

Research for Eexperimental System of Differential Isodyne

The .experimental system of differential Isodyne developed by authors is represented. The function and structure characteristic of the rotatable real-time loading device which is set in oil tank , the hydraulic scanning device and the device for synchronous movement of incident beam and Isodyne collector are mainly discussed. The fringe pattern of differential Isodyne in various characteristic directions and planes can be real-timely taken, by using this system. This system is able to apply to stress analyses of local effect .composite materials and structures.     

A New Monte Carlo Method Based on State Analysis

A new Monte Carlo Method for system reliability evaluation is proposed in this paper. The repeated sampling of system states with relatively great probabilities is avoided in the simulation,thus the sampling efficiency is significantly improved. Besides,the negative correlations used in different trials can further reduce the estimated varinaces. The sparse matrix technique is employed to reduce the memory requirement and to save the computer time. Comparative studies show that the method proposed is of great value and the application to the IEEE reliability test system (power system) is successful.     

STATE VARIABLE METHOD FOR DYNAMIC SIMULATION OF HVDC SYSTEMS

A flexible mathematical model used for dynamic simulation studies of HVDC systems which is based on the stale variable method is presented, and the correspongding computer program has been implemented. Generality, easy to use, and suitable to run on microcomputers are the main features of the model and the computer program. Therefore the model is easy to be extended for application. An example is given in the paper to verily the coneclness of the model and the program.     

Management System of the Tender and Bidding for Power Projects with a Valuation Based on the Bill of Quantities

Valuation based on the bill of quantifies will change the existing valuation mode of tender and bidding. For the standardized management of tender and bidding for power projects and the inteUigentized information management of power enterprises, it is an inevitable trend of reform to apply the valuation mode based on the bill of quantifies. According to the characteristics of power projects, this paper expounds the working process of the valuation mode based on the bill of quantities and analyzes the goal and function need of the tender and bidding management system. Considering the calculation procedure of the valuation and the quote strategy of quantities bill, the composition of fees for the valuation is settled and the calculation method is designed. To establish the management system of tender and bidding with a valuation based on the bill of quantities is beneficial for the costing reform of power projects and the perfection of the tender and bidding market. Therefore it is of great significance to spread the new management mode of project costing with a valuation based on the bill of quantities.