商洛市平菇栽培及病虫害防治关键技术

商洛市作为我国平菇栽培的重要产区之一,拥有超过50年的平菇人工栽培历史,商洛地区平菇产业不仅在农业经济方面取得显著成就,而且深受市场和消费者喜爱。本文系统总结了近年来商洛市平菇的栽培技术及病虫害防治策略。详细介绍了商洛市平菇目前主推的栽培的季节及品种选择、栽培配方、制袋、养菌管理、出菇管理、采收等内容,分析了平菇的常见病虫害,并提出了相应的防治措施。这项研究为商洛地区平菇产业的发展壮大提供了技术支持。     

不结球白菜形态性状及SSR遗传多样性关联分析

分析不结球白菜遗传多样性,筛选与形态性状相关联且具有多态性的标记位点,为不结球白菜的分子辅助育种选择、种质资源的利用和新品种选育提供依据.本研究以类型差异大、有南方特色的54份种质资源为材料,对其6个质量性状和5个数量性状进行3次田间调查,从40对引物中筛选出25对SSR引物进行遗传多样性分析,结合形态性状对54份不结...     

用SSR标记初步分析高州普通野生稻的籼粳分化

利用34对SSR引物对高州普通野生稻(简称"高野",下同)7个自然居群217份个体进行研究,并结合统计软件进行遗传距离和遗传结构分析.通过已明晰籼、粳类型的中国栽培稻微核心种质作对照检测,所用引物能有效区分籼粳亚种,并在高野材料中扩增出籼稻带型、粳稻带型以及野生稻特殊带型.结果表明,高野群体总体比较原始,已经出现了籼粳分化,其中偏粳多于偏籼,但这种分化是初步的.本研究为进一步明确高野在中国栽培稻的起源演化上的地位、并对其有效的发掘利用提供科学参考.     

神农架大九湖生物多样性及湿地恢复技术探讨

大九湖湿地是湖北省境内海拔最高的高山湿地。笔者研究了大九湖的生物多样性,并对其进行了评价,提出了湿地的恢复技术。     

Identification of a conservative site in the African swine fever virus p54 protein and its preliminary application in a serological assay

BackgroundASF was first reported in Kenya in 1910 in 1921. In China, ASF spread to 31 provinces including Henan and Jiangsu within six months after it was first reported on August 3, 2018. The epidemic almost affected the whole China, causing direct economic losses of tens of billions of yuan. Cause great loss to our pig industry. As ELISA is cheap and easy to operate, OIE regards it as the preferred serological method for ASF detection. P54 protein has good antigenicity and is an ideal antigen for detection.ObjectiveTo identify a conservative site in the African swine fever virus (ASFV) p54 protein and perform a Cloth-enzyme-linked immunosorbent assay (ELISA) for detecting the ASFV antibody in order to reduce risks posed by using the live virus in diagnostic assays.MethodWe used bioinformatics methods to predict the antigen epitope of the ASFV p54 protein in combination with the antigenic index and artificially synthesized the predicted antigen epitope peptides. Using ASFV-positive serum and specific monoclonal antibodies (mAbs), we performed indirect ELISA and blocking ELISA to verify the immunological properties of the predicted epitope polypeptide.ResultsThe results of our prediction revealed that the possible antigen epitope regions were A23–29, A36–45, A72–94, A114–120, A124–130, and A137–150. The indirect ELISA showed that the peptides A23–29, A36–45, A72–94, A114–120, and A137–150 have good antigenicity. Moreover, the A36–45 polypeptide can react specifically with the mAb secreted by hybridoma cells, and its binding site contains a minimum number of essential amino acids in the sequence 37DIQFINPY44.ConclusionsOur study confirmed a conservative antigenic site in the ASFV p54 protein and its amino acid sequence. A competitive ELISA method for detecting ASFV antibodies was established based on recombinant p54 and matching mAb. Moreover, testing the protein sequence alignment verified that the method can theoretically detect antibodies produced by pigs affected by nearly all ASFVs worldwide.     

基于“三生”空间视角的中国农业文化遗产研究文献分析与评述

本研究基于"生产、生活、生态"的空间理论视角,揭示中国农业文化遗产"三生"空间人地关系的研究现状。以"农业文化遗产"为关键词,采用Citespace5.5文献计量分析工具和归纳方法,梳理了近年来中国农业文化遗产的研究成果,结果表明:2005—2020年中国农业文化遗产的研究经历了初步探索、波动增长、平稳发展三个阶段,各个阶段上的研究热点具有明显差异。目前中国农业文化遗产在景观价值挖掘、旅游开发、乡村振兴等领域的研究成果已初具规模,但在基础性理论方面尚未形成权威的认知。农业文化遗产在"三生"空间上的研究对于解决"三农"问题具有重要的实践意义,今后应更加关注基础理论的更新、强化实证研究、贯彻"分层"保护思维、完善社区参与、加快农业文化遗产地的评价指标体系构建和动态保护,以期实现农业文化遗产的高效利用及可持续发展。     

樱桃小茎尖培养后的早熟变异与RAPD鉴定

樱桃品种Ｘｅｓｐｈｙｅ（Ｐｒｕｎｕｓ　ａｖｉｕｍ）的休眠芽通过小茎尖分生组织培养后,发现一株组培苗结果后成熟期明显早于Ｘｅｓｐｈｙｅ及同批其它试管苗,该株经无性繁殖试栽后早熟性状稳定,并且综合性状良好,具栽培价值,暂定名“早丹”。经ＲＡＰＤ技术鉴定,该早熟变异株的ＤＮＡ指纹图谱与原Ｘｅｓｐｈｙｅ品种不同,早熟变异株比原 Ｘｅｓｐｈｙｅ品种多扩增一条 ＯＰＵ０３－９５０ ＤＮＡ谱带,说明该株系发生了ＤＮＡ的变异。     

早熟梨新品种区域性比较试验

七月酥、早美酥是分别用幸水×早酥和新世纪×早酥杂交培育而成。区域性试验研究表 明二者具有早果、丰产、优质的特性,在外观、色泽、品质等方面优于我国目前广泛栽培的早酥梨, 是有推广前途的极早熟或早熟梨新品种。     

聚类分析在我国果树研究中的应用及问题分析

对近１０年来聚类分析在我国果树研究中的应用情况进行了简要总结。涉及十余种果树及系统聚类和模糊聚类两种聚类分析方法。文中对有关问题进行了分析和讨论。