Multi-time scale analysis of sugarcane within-field variability: improved crop diagnosis using satellite time series?

Within-field spatial variability is related to multiple factors that can be time-independent or time-dependent. In this study, our working hypothesis is that a multi-time scale analysis of the dynamics of spatial patterns can help establish a diagnosis of crop condition. To test this hypothesis, we analyzed the within-field variability of a sugarcane crop at seasonal and annual time scales, and tried to link this variability to environmental (climate, topography, and soil depth) and cropping (harvest date) factors. The analysis was based on a sugarcane field vegetation index (NDVI) time series of fifteen SPOT images acquired in the French West Indies (Guadeloupe) in 2002 and 2003, and on an original classification method that enabled us to focus on crop spatial variability independently of crop growth stages. We showed that at the seasonal scale, the within-field growth pattern depended on the phenological stage of the crop and on cropping operations. At the annual scale, NDVI maps revealed a stable pattern for the two consecutive years at peak vegetation, despite very different rainfall amounts, but with inverse NDVI values. This inversion is linked with the topography and consequently to the plant water status. We conclude that (1) it is necessary to know the crop growing cycle to correctly interpret the spatial pattern, (2) single-date images may be insufficient for the diagnosis of crop condition or for prediction, and (3) the pattern of vigour occurrence within fields can help diagnose growth anomalies.

Preserved feedforward but impaired top-down processes in the vegetative state

Frontoparietal cortex is involved in the explicit processing (awareness) of stimuli. Frontoparietal activation has also been found in studies of subliminal stimulus processing. We hypothesized that an impairment of top-down processes, involved in recurrent neuronal message-passing and the generation of long-latency electrophysiological responses, might provide a more reliable correlate of consciousness in severely brain-damaged patients, than frontoparietal responses. We measured effective connectivity during a mismatch negativity paradigm and found that the only significant difference between patients in a vegetative state and controls was an impairment of backward connectivity from frontal to temporal cortices. This result emphasizes the importance of top-down projections in recurrent processing that involve high-order associative cortices for conscious perception.     

Saving the small farm: Agriculture in roman literature

Roman agriculture suffered traumatic changes during the 2nd century B.C. The traditional farmers who tilled their few acres and served family, gods and community were being squeezed out by large estate owners using slaves for investment farming. Politicians, scholars and poets tried to revive the ancestoral rustic life. In 133 B.C. the Gracchi legislated land reform to relieve the distress of the farmer soldiers who had won the empire. Although their efforts led to political confrontation that deteriorated into civil war, programs for the traditional farm became a permanent part of government policy from the late Republic until the end of the empire in 476 A.D. Scholars and poets made a contribution to the revival of agriculture with knowledge for improving the farm and by encouraging an agrarian mentality. The agricultural manuals (e.g. Cato (c. 150 B.C.), Varro (c. 50 B.C), and Columella (c.65 A.D.), defined the nature of the desirable farm and gave practical advice. Profit was the goal, but good farming practices made for pleasure and virtue as well. The image of the ancestoral farmer was perpetuated as was the notion that farming was the only honorable and respectable occupation for a Roman gentleman. In the Augustan Age (34 B.C.—14 A.D.) poets were encouraged by the government to adopted a rustic theme in hopes it would stimulate a return to the land and aid in the rebirth of Rome and Romans. In the GeorgicsVirgil begins with the practical details of farming, but uses myth and philosophy to explore the nature and meaning of life. He admits that Jove made life perilous. But Jove also gave man the art of agriculture and with hard work man could know the pleasure of a simple, virtuous, productive life. Horace directed his poetry against the allure of city life and in praise of rustic living. Epicureanism and Stoicism, in the guise of life on the farm, could show that although fate was unpredictable, the world was orderly and, if one recognized and accepted its limits, one could make a garden of the world and live a simple but happy life. By 300 A.D. Rome missed the peasant-farmer-soldier and by the 470's life had returned to an agrarian condition. Bishop Sidonius, trying to furnish meaning and perspective for the emerging new age, resorted to the Roman agricultural traditions still cherished as that world disappeared.     

Bypass of DNA lesions generated during anticancer treatment with cisplatin by DNA polymerase eta

DNA polymerase eta (Pol eta) is a eukaryotic lesion bypass polymerase that helps organisms to survive exposure to ultraviolet (UV) radiation, and tumor cells to gain resistance against cisplatin-based chemotherapy. It allows cells to replicate across cross-link lesions such as 1,2-d(GpG) cisplatin adducts (Pt-GG) and UV-induced cis-syn thymine dimers. We present structural and biochemical analysis of how Pol eta copies Pt-GG-containing DNA. The damaged DNA is bound in an open DNA binding rim. Nucleotidyl transfer requires the DNA to rotate into an active conformation, driven by hydrogen bonding of the templating base to the dNTP. For the 3'dG of the Pt-GG, this step is accomplished by a Watson-Crick base pair to dCTP and is biochemically efficient and accurate. In contrast, bypass of the 5'dG of the Pt-GG is less efficient and promiscuous for dCTP and dATP as a result of the presence of the rigid Pt cross-link. Our analysis reveals the set of structural features that enable Pol eta to replicate across strongly distorting DNA lesions.