肺癌腔内后装治疗方法改进的研究

对７０例肺癌患者随机分为两组，用改进麻醉及插管方法与常规方法作了对经研究，结果表明：在改进法组中，患者剧烈咳嗽，支气管痉挛和咯血等并发症明显减少，并对两组并发症的治疗作了讨论。     

Probing gene expression in live cells, one protein molecule at a time

We directly observed real-time production of single protein molecules in individual Escherichia coli cells. A fusion protein of a fast-maturing yellow fluorescent protein (YFP) and a membrane-targeting peptide was expressed under a repressed condition. The membrane-localized YFP can be detected with single-molecule sensitivity. We found that the protein molecules are produced in bursts, with each burst originating from a stochastically transcribed single messenger RNA molecule, and that protein copy numbers in the bursts follow a geometric distribution. The quantitative study of low-level gene expression demonstrates the potential of single-molecule experiments in elucidating the workings of fundamental biological processes in living cells.     

中间罐尾气回收装置的运行及其改进措施

针对金陵分公司油品储运部中间罐区储存含有H2S粗汽油、粗柴油中间料挥发外泄带来的环境问题,采用抚顺石化研究院开发的“液态吸收”法对中间储罐排出的含硫、含烃尾气进行有效吸收:经冷冻的粗柴油作为贫吸收油,吸收尾气中的烃类组分,再经30％碱液重力反应器吸收含硫物质,尾气经上述处理过程后实现达标排放.通过对运行中存在的系统压力、吸收剂温度、碱液浓度等问题进行分析,提出了相关改进措施.改进后的装置运行稳定,连续尾气吸收效果较好.     

两种低氧条件对赤拟谷盗不同虫态的抑制作用

研究了在28℃、75%RH的温湿度条件下,5%和2%低氧储藏条件对赤拟谷盗高龄幼虫、蛹和成虫的抑制作用。在5%和2%氧气条件下暴露15d后,赤拟谷盗高龄幼虫化蛹率分别为95.0%和3.7%,蛹羽化率分别为98.0%和0%,成虫死亡率分别为12.7%和100%。本研究结果为气调储粮防治害虫提供依据。     

广东割手密SSR遗传多样性分析

割手密是甘蔗近缘野生种之一,在甘蔗育种中具有重要地位和作用。本研究采用Genomic-SSR和EST-SSR两种类型分子标记对来自广东各个地区的67个割手密种质进行分析。在20对SSR引物上共检测到341个多态性条带,每个引物上的平均多态性为17.05,其中11个Genomic-SSR的平均多态性为20.82,9个EST-SSR的平均多态性为12.44。广东割手密在Genomic-SSR上的多态性显著高于EST-SSR上的多态性。MantelTest分析表明:通过Genomic-SSR、EST-SSR以及Genomic-SSR+EST-SSR三种方法所计算的试验材料的遗传距离矩阵之间都呈极显著相关(p<0.01),说明应用两种类型SSR获得的各个材料间的遗传关系具有较高的一致性。广东割手密在20对SSR引物上的遗传多样性指数介于0.1064~0.2916之间,平均多样性指数为0.1943;各个材料间的遗传距离为0.0063~0.3082之间,平均为0.1935。UPGMA聚类分析表明:广东割手密之间的遗传关系和地理来源存在一定的相关,由南向北存在较为显著的地理分化。     

An integrated approach of remote sensing,GIS and swarm intelligence for zoning protected ecological areas

Interest in protecting ecological areas is increasing because of land uses conflicts and environmental pressures. The optimal zoning of protected ecological areas belongs to a NP-hard problem because it is subject to both box and spatial constraints. A challenge in solving area optimization problems emerges with the increasing size of a study region. In this article, an integrated approach of remote sensing, GIS and modified ant colony optimization (ACO) is proposed for application in zoning protected ecological areas. Significant modifications have been made in the conventional ACO so that it can be further extended to solve zoning problems in large regions. An improved selection strategy is designed to accelerate the progress of sites selection for artificial ants. Another important modification in ACO is to incorporate the neighborhood diffusion strategy into pheromone updating. The optimal objective is to generate protected areas that maximize both ecological suitability and spatial compactness. The modified ACO model has been successfully applied to a case study involving an area of 25,483 cells in Dongguan, Guangdong, China. The experiments have demonstrated that the proposed model is an efficient and effective optimization technique for generating optimal protection. The modified ACO model only requires approximately 119 s for determining near-optimal solutions. Furthermore, the proposed method performs better than other methods, including simulated annealing, genetic algorithm, iterative relaxation, basic ACO, and density slicing.     

不同氮肥运筹方法对免耕抛秧稻产量影响的对比试验

免耕抛秧晚稻在氮、磷、钾肥施用量相同条件下．氮肥以基肥占20％、立苗后第一次追肥占30％、分蘖肥占20％、穗前肥占30％的运筹方法较适宜免耕抛秧稻前缓中快后稳等生长发育特点的需要,可为穗大粒多打下良好基础,从而获得较高的产量。     

Technical knitting and ergonomical design of 3D seamless compression hosiery and pressure performances in vivo and in vitro

The present study was conducted from a new and innovative view to analyze pressure performances of elastic compression hosieries (ECHs) based on a technical knitting and ergonomic design and development methodology. ECHs with pressure classes I and II have been designed and developed. Multiple influencing factors and their dynamic interaction involved in the working mechanisms of ECHs were analyzed and discussed in this study. Yarn properties, material combinations, knitting structural complex, and parameters setting of machinery elements determine the fabric elasticity, stiffness, hosiery configuration, pressure magnitudes and gradient distribution. A good agreement and significant correlation on pressure performances of the developed hosieries are present from positions B1 to D when being tested on the wooden leg model by using Medical stocking tester in vitro and on the studied subjects’ legs in vivo. A reasonable pressure gradient was presented on the leg model (i.e. 70–100 % pressure at B1 and 50–80 % at C compared with the pressure exerted at ankle B), which supported the technical knitting design methodology applied in this study. However, some differences at ankle region B existed between the in vivo and in vitro values due to differences of anatomic structures that existed on the tested ankle areas. The location of testing points and body postures being undertaken by the wearers also significantly influenced the pressure profiles in vivo. More stretch deformations in hosiery fabric are produced, as assessed by static stiffness index evaluation, at the ankle when the body posture changes from standing to supine. The developed ECHs with pressure classes I and II received positive feedback on pressure and comfort perceptions on testing spot. More explorations are needed to elevate the consistency of the in vitro and in vivo pressure values at ankle region and to investigate their long-term pressure performances in practical wear through innovative knitting technical design with considerations of ergonomic factors and conducting larger scale of wear trials in future studies.     

牦牛MYL3基因的克隆及组织表达分析

【目的】研究牦牛MYL3基因的结构和表达特征,探究其生物学功能及影响牦牛肌肉生长发育的机制。【方法】以美仁牦牛cDNA为模板,PCR扩增MYL3基因编码区序列(CDS),利用MEGA11软件构建系统进化树,利用生物信息学软件对其编码蛋白进行分析。通过实时荧光定量PCR(RT-qPCR)检测MYL3基因在心脏、肝脏、脾脏、肺脏（参照）、睾丸、肌肉和脂肪组织中的相对表达量。【结果】牦牛MYL3基因CDS区长600 bp,共编码199个氨基酸,存在1个碱基突变位点,位于第165位(A>T)。系统进化分析结果显示,牦牛与普通牛的亲缘关系最近,与单峰驼的亲缘关系最远。生物信息学分析结果显示,牦牛MYL3蛋白为不稳定的亲水性蛋白,无信号肽,不存在跨膜结构,主要存在于细胞质内,有8个磷酸化位点和2个N 糖基化位点,蛋白的二级结构以α-螺旋为主,主要与调控肌肉生长发育的相关蛋白相互作用。RT-qPCR结果表明,MYL3基因在心脏中的表达量最高,极显著高于其他组织;肌肉中次之,与除心脏外的其他组织存在极显著差异。【结论】MYL3基因可能与牦牛心脏发育有关,对肌肉的生长发育和肉质有一定影响。     

8个格木天然林种源苗期生长差异与早期选择

为研究格木不同天然种源家系苗的生长差异,选出相对速生的优良种源,本研究对8个格木天然林种源、110个家系1年生的苗高和地径等生长量指标进行测量,结合各家系的种子表型性状进行方差分析、多重比较、相关性分析和聚类分析。结果表明:不同种源的格木苗期生长量差异显著,具备种源选择的基础;不同种源的苗期生长量差异主要来源于种源内,具备从优良种源内选择优良家系的基础;苗期生长量与种子大小、种子厚和千粒重等性状呈极显著正相关关系,大粒、饱满种子可作为优良种源和家系选择的初选参考指标。初步选出浦北、北流种源作为格木优良种源。本研究为格木速生种源及家系的早期选择提供依据。