鸡沙门氏菌弱毒冻干苗的研制

由减毒鸡沙门氏苗97A疫苗株作为制苗用菌种，经普通琼脂培养，冷冻真空干燥等工艺生产鸡沙门氏菌弱毒疫苗4批，每批疫苗分别以免疫剂量接种6日龄AA肉鸡，免疫第14天时，用强毒株Sg9和Sp4攻击，免疫组死亡保护率均达90％以上，试验结果表明，该苗具有良好的安全性和免疫效果。     

Development of Chinese Near-isogenic Line of Rice and Their Differentiating Ability of Pathogenic Races of Blast Fungus

The rice( Oryza L. ) variety, Lijiangxintuanheigu(LTH), is a universally susceptible variety. A set of near-isogenic lines (NILs) of rice which has single resistance gene for each line was developed by use of LTH as recurrent parent and Kusabue,Tsuyuake, K1, PiNo. 4, K60 and BL1, which are from Kiyosawa‘s differential varieties(KDV), as donor parents. Differentiating ability of these NILs to Philippine isolates was compared with that of their donor parents, KDV and IRRI‘ s NILs. The results of comparative studies were as follows: (1) Differentiating ability of Chinese NILs to Philippine isolates was much stronger than that of their donor parents and that of KDV. (2) Chinese NILs had similar ability to differentiate races to IRRI‘s NILs or higher differentiating ability than them. But IRRI‘s NILs almost had no differentiating ability to isolates from japonica rice-growing regions. They can not be used to differentiate races of isolates from japonica ricegrowing regions. Whereas, Chinese NILs can be used to differentiate races of isolates from both japonica ricegrowing regions and indica rice-growing regions. It was concluded that the set of Chinese NILs can be used as international differentials.     

振动深松鼠道犁的试验分析

石河子及周围垦区的土壤粘重且呈盐碱性,由于犁底层的存在,土质很难得到改善。而深松作业则是打破犁底层的主要农业技术措施之一。为此,论述了IZSS-600-3型振动深松鼠道犁的构造和工作原理,分析了试验因素对试验指标的影响,为机具今后在农业生产中的应用和推广提供了依据和参考。     

In vitro Plant Regeneration from the Mature Tissue of Navel Orange (Citrus sinensis L. Osbeck) by Direct Organogenesis

An efficient in vitro regeneration system by direct organogenesis from mature nodal and internodal stem segments of Newhall navel orange (Citrus sinensis L. Osbeck) was developed. Illuminating conditions together with plant growth regulators affected the adventitious bud regeneration frequency and efficiency. The initial 15 d darkness inoculation is beneficial for the adventitious bud regeneration. The highest regeneration frequency (85.2%) and bud formation efficiency (3.7 per responsive internodal stem segment) were obtained in the media supplemented with 1.0 mg L-1 BAP and 0.5 mg L-1 NAA. ABA at 0.2 mg L-1 positively affected the bud formation efficiency, which amounted to 8.5 buds per internodal segment in the presence of BAP at 1.0 mg L-1. The adventitious shoots successfully rooted and were transferred to the soil.     

近交系大鼠DNA指纹图与微卫星DNA比较分析

为了建立一种对近交系大鼠遗传物质进行精确可靠、快速简便的监测方法,利用DNA指纹技术对国内6个品系8个近交系大鼠群体进行了DNA多态性的分析,并与PCR扩增微卫星DNA技术进行了比较.其结果显示(1)不同品系之间DNA指纹图差异较大,同一群体不同个体间DNA指纹图带的相似系数和共有带率除SHR(哈)和WKY(哈)小于0.7外,其他均大于0.9.不同地区同一SHR间和WKY间DNA指纹图也存在差异,相同DNA不同次制作的DNA指纹图谱基本一致.(2)不同品系个体间微卫星DNA具有显著多态性;同一群体不同个体之间除SHR(哈)的SMST位点和WKY(哈)的AGT位点出现一定的差异外,其他均没有差异.DNA指纹图能更精确可靠地反映出动物个体间的遗传背景,而微卫星DNA遗传监测方法比较简便快捷.     

丝核菌细胞核染色技术的研究

比较了5种不同的染色方法对丝核菌（Rhizoctonia)菌株细胞核的染色效果。结果表明，番红O－KOH的染色效果最好，Giemsa、苯胺蓝－甘油次之，染色效果最差的是苯胺蓝－乳酚油及曲利苯蓝－乳酚油。用番红O－KOH染色方法对分离自13种作物的57个丝核菌菌株进行细胞核染色，其中3个菌株为双核丝核菌，其余54个菌株为多核丝核菌。     

表达HAV结构基因的重组腺病毒鉴定及细胞病变分析

利用RT-PCR方法,从HAV-L8株的RNA中扩增出结构基因(vp3 vp1),克隆到穿梭质粒pXCX2NotI上。采用磷酸钙-DNA共沉淀技术,将腺病毒载体pJM17与pXCX2-CMV-HAV共转染293细胞。在光学显微镜下观察到明显的细胞病变(CPE);经RT-PCR、免疫荧光染色和蛋白印迹鉴定证明HAV的结构基因已插入到腺病毒基因组中;在电子显微镜下观察发现有二十面体的病毒颗粒。以上结果表明获得了重组腺病毒rAdHAV,纯化后的rAdHAV滴度为1×109.0TCID50/mL。     

仔兔补饲方法的比较试验

仔兔从18～20日龄采取随笼补饲和离笼补饲两种方法。两批试验结果表明:随笼补饲的仔兔断奶窝重、窝日增重及成活率均明显高于离笼补饲的仔兔,重要的是随笼补饲极大简化了烦琐操作程序,减少了工作量,更适合规模化养兔场应用。     

立地管理措施对7年生2代杉木林生长的影响

对福建省南平峡阳国有林场29年生1代杉木人工林采伐迹地,采取5种不同立地管理措施营造的第2代杉木人工林7年生时的生长量进行调查分析,结果表明,BL3(收获树干和树皮,加倍采伐剩余物)处理对7年生2代杉木林的生长最为有利,其次为BL2(收获树干和树皮,不炼山)处理、BL1(全树收获)处理和BL0(收获地上所有有机质)处理,而SB(收获树干和树皮,炼山)处理杉木生长最差。炼山处理的7年生杉木蓄积量仅为不炼山处理的85.73%,但不同试验处理之间杉木生长量的差异均未达到显著水平(P<0.05)。     

农机化装备结构多目标优化的模糊综合评判方法

通过对农业机械化装备结构影响因素的分析研究．从农业产品产业结构、农业投入产出水平、农业农艺农作条件、农业生产组织形式和农业技术人才资源五个方面建立评价指标体系，提出农业机械化装备结构多目标优化的模糊综合评判模型，为农机化规划巾装备结构的优化提供一种科学的方法和决策依据。