4种外源激素处理对羊草种子萌发和幼苗生长的影响

研究了100~600μg/g赤霉素(GA3)和吲哚乙酸(IAA)、25~100μg/g 6-苄氨基嘌呤(6-BA)和脱落酸(ABA)对羊草种子萌发和幼苗生长的影响。结果表明,100~300μg/g GA3和IAA以及25μg/g 6-BA对羊草种子的萌发最终表现为促进作用,总体效果为GA3>IAA>6-BA。当GA3和IAA浓度大于400μg/g或6-BA浓度大于50μg/g时对羊草种子的最终发芽率均表现为不同程度的抑制;25~100μg/g的外源激素ABA对羊草种子萌发没有抑制作用。GA3处理均能显著促进羊草幼苗的伸长,IAA和6-BA则表现为不同程度的抑制作用;GA3、IAA和6-BA 3种促进激素对羊草的根长和根冠比均表现为抑制作用;25~100μg/g ABA对羊草苗长、根长以及根冠比没有显著影响。     

石蝉草乙醇提取物除草活性初探

为探明石蝉草是否具有除草活性,本研究采用小杯法和盆栽试验法测定石蝉草乙醇提取物对13种植物的除草活性。结果表明,石蝉草乙醇提取物对13种植物的根茎生长均有抑制作用,对苘麻和稗草根长的抑制中浓度IC50分别为0.73和0.80mg/mL,对茎长的IC50分别为0.77和0.89mg/mL;盆栽试验表明,5mg/mL浓度对稗草和苘麻7d的防治效果分别为90.73%和82.02%,与对照药剂癸酸无显著性差异。研究结果为研发新型除草剂提供了新思路。     

黑龙江省大豆疫霉根腐病调查与病原分离

１９９６年对黑龙江省东部和中部大豆产区２３个市、县的大豆苗期疫霉根腐病进行了调查、研究,应用ＰＢＮＩＣ疫霉选择性培养基分离大豆疫霉根腐病病原菌,从牡丹江、穆棱、林口和佳木斯豆田具疫霉根腐症状的大豆植株上分离到大豆疫霉根腐病菌,并从根腐病株上单独或与大豆疫霉菌同时分离到终极腐霉菌,研究进一步证实我国黑龙江省有大豆疫霉根腐病。调查发现,大豆疫霉根腐病和终极腐霉根腐病主要发生在土质粘重、土壤含水量高或易积水的田块。     

马铃薯甲虫空间分布型及序贯抽样

马铃薯甲虫Leptinotarsa decemlineata Say是一种重要的害虫,为明确其在新疆的空间分布,及时采取有效的防控措施,在田间调查的基础上,采取随机取样和Iwao回归分析法对新疆乌鲁木齐地区的马铃薯甲虫成虫、卵块和幼虫的空间分布型、理论抽样数及幼虫的序贯抽样方法进行了研究。结果表明,马铃薯甲虫各虫态空间格局的聚块性指标均大于1,空间分布型为普通群聚型;对低龄幼虫进行序贯抽样,当防治阈值为50头/样方、置信水平为1.96时,防治上、下限方程分别为:d1=50n+81n和d0=50n-81n,当百株虫量达到756头以上时需要进行防治。     

Investigation of the In Vivo,In Vitro,and In Silico Wound Healing Potential of Pinctada martensii Purified Peptides

Previous studies found that both oral and topical administration of enzymatic digestion products < 3 K Da ultrafiltration fractions of Pinctada martensii mantle (PMPs) had pro-healing effects. Thus, we further purified them by Sephadex-G25 and screened them by cellular assays to obtain Pinctada martensii purified peptides (PMPPs). In this study, we explored the mechanism of PMPPs on wound healing by in vivo, in vitro, and in silico experiments. LC-MS/MS results showed that PMPPs consisted of 33 peptides with molecular weights ranging from 758.43 to 2014.04 Da, and the characteristic peptide was Leu-Asp. The results of cellular assays showed that PMPPs promoted the proliferation of human skin fibroblasts (HSF) (135%) and human immortalized keratinocyte (HaCaT) cells (125%) very significantly at 12.5 μg/mL. The in vivo results showed that PMPPs could achieve scarless healing by inhibiting the inflammatory response, accelerating the epithelialization process, and regulating collagen I/III ratio. The optimal peptide sequence FAFQAEIAQLMS of PMPPs was screened for key protein receptors in wound healing (EGFR1, FGFR1, and MMP-1) with the help of molecular docking technique, which also showed to be the key pro-healing active peptide sequence. Therefore, it may provide a therapeutic strategy with great potential for wound healing.     

SIRT1 promotes autophagy of pancreatic cancer cells induced by hypoxia via regulating FOXO1/RAB7 signaling pathway

AIM: To investigate the effect of SIRT1 on the autophagy of pancreatic cancer cells under hypoxia condition, and to analyze the underlying mechanism of regulating FOXO1/RAB7 signaling pathway. METHODS: Western blot and immunofluorescence methods were used to determine the expression of SIRT1 in the pancreatic cancer cells. The small interfering RNA targeting SIRT1 and SIRT1 over-expression plasmid were transfected into the pancreatic cancer Panc-1 cells. Confocal microscopy was used to detect the LC3 expression. Western blot was used to analyze the protein levels of LC3, p62 and FOXO1/RAB7 signaling pathway-related molecules. Co-immunoprecipitation was used to detected the protein interaction between SIRT1 and FOXO1. RESULTS: The expression level of SIRT1 in the nucleus of Panc-1 cells was increased under hypoxia condition. Compared with negative control under hypoxia condition, knock-down of SIRT1 expression attenuated the autophagy flux in the pancreatic cancer Panc-1 cells (P<0.05). Over-expression of SIRT1 increased the protein levels of FOXO1 and RAB7. On the contrary, knock-down of SIRT1 expression inhibited the protein levels of FOXO1 and RAB7. The protein interaction between SIRT1 and FOXO1 in the pancreatic cancer cells was observed. CONCLUSION: SIRT1 in pancreatic cancer Panc-1 cells under hypoxia condition is over-expressed in the nucleus. Down-regulation of SIRT1 inhibits autophagy and its mechanism may be related to FOXO1/RAB7 signaling pathway.     

生根粉在八仙花扦插繁殖中的应用研究简

在八仙花（Hydrangea macrophylla）扦插繁殖中,使用不同浓度的用生根粉配制的溶液,并进行不同施用方式及不同施用时间的处理。试验结果表明,合理使用生根粉,能够有效地提高八仙花扦插繁殖的生根率。     

我国草地贪夜蛾的防控对策与建议

草地贪夜蛾Spodoptera frugiperda(J.E.Smith)是世界性重大农业害虫,该虫自2019年1月入侵我国云南省到5月下旬,在不到5个月的时间里就已经入侵了长江流域及其以南的15个省(市、自治区)。预计6月和7月将到达我国的玉米主产区黄淮海夏玉米区和北方春玉米区,受威胁玉米面积1 300多万hm 2,将严重威胁我国的玉米生产安全。我国玉米的种植布局随季节和纬度变化从南至北递次推移,时间和空间上互补,为草地贪夜蛾提供了丰富的食物资源,为其种群区域性迁移和周年繁殖为害提供了良好的自然条件。为做好草地贪夜蛾的防控工作,及时控制其为害,本文在总结国内外防控草地贪夜蛾经验教训的基础上,初步提出了我国草地贪夜蛾的防控对策。     

云南高海拔粳稻区白叶枯病菌致病型的鉴定

水稻白叶枯病是危害水稻的重要细菌性病害之一,病原为稻生黄单胞菌水稻致病变种Xanthomonas oryzae pv.oryzae (Xoo).近10年来云南省水稻白叶枯病的危害向高海拔粳稻区扩散,在海拔2200m的剑川县,白叶枯病已成为最严重的水稻病害[1-2].开展高海拔粳稻区白叶枯病菌致病型的鉴定、分布和分化研究,对云南高海拔粳稻区稻作生产具有指导意义.     

龟甲冬青枝枯病及其病原菌鉴定

于2002-2005年连续3年观察了杭州龟甲冬青枝枯病的症状,该病害先在嫩枝枝条发病,病斑初为水渍状斑点,后扩大成暗褐色的不规则形病斑。从发病部位分离培养出病原菌,根据其分生孢子的形态、大小和核糖体DNA内转录间隔区的序列,鉴定为褐纹拟茎点霉［Phomopsis vexans (Sacc.et Syd.) Harter］。本文对该病害症状和病原形态特征进行了描述。