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11.
Yehoram Leshem Avinoam Nerd 《The Journal of Horticultural Science and Biotechnology》2013,88(3):426-431
SummaryFruit of Cereus peruvianus (L.) Miller, a newly developed cactus crop, is subject to cracking. Fruit growth follows a double sigmoid curve, with a significant size increase in the first and third stages and moderate increase in the middle stage. Peel and seed grow in the first two stages, while pulp growth and maturation (e.g. peel colour change and stretching, sugar accumulation, fruit softening) occur in the final stage. Sensitive, moderately sensitive, and tolerant clones are subject to 100, 50–60 and 6–9% cracking, respectively, correlated to relative growth rate of the pulp in the third stage. Fruit cracking, lengthwise splitting of the peel, and exposure of the pulp occurred in fully mature fruits towards the end of their growth. Continuous monitoring of fruit growth revealed expansion and cracking during the day and shrinkage during the night, as expected for CAM plants. Cracking was not correlated with the threshold cracking turgor, which declined during ripening in all clones. GA3 sprays during colour break reduced cracking in the two most susceptible clones to 20% and 60%, while paclobutrazol increased cracking. Cracking rates were the same in two ecozones differing in salinity, temperatures and relative humidity. It is suggested that breeding and hormone treatments could reduce cracking significantly. 相似文献
12.
J T van Oirschot F Daus T G Kimman D van Zaane 《American journal of veterinary research》1991,52(11):1788-1793
To study the antibody response to glycoprotein I (gI) of pseudorabies virus (PRV) in maternally immune pigs, 3 groups of 6 pigs were given low doses of the mildly virulent Sterksel strain of PRV at 3 and 11 weeks of age. Group A consisted of seronegative pigs; groups B and C consisted of pigs with maternal antibodies deficient of antibodies to gI. At 3 weeks of age, 3 pigs of each group were inoculated intranasally with 10(2.5) plaque-forming units (groups A and B), or with 10(3.5) plaque-forming units (group C) of PRV. The 3 other pigs in each group were contact-exposed to the inoculated pigs. In group A, 4 of 6 pigs shed virus and all developed antibodies to gI of PRV and produced PRV-specific IgM and virus-neutralizing antibodies. In groups B and C, 10 pigs shed virus and all developed low and inconsistent titers of gI antibodies, whereas only 3 pigs produced PRV-IgM antibodies with low titers. Thus, after PRV infection of pigs with high concentrations of maternal antibodies deficient of gI antibodies, the antibody responses to PRV were severely inhibited. The pigs were reinoculated with 10(3) plaque-forming units of the same virus 8 weeks after the first inoculation. The pigs in group A did not respond at all, as they were immune. The pigs in groups B and C shed considerable amounts of virus. Three pigs had a clear secondary antibody response to gI, whereas the others developed an early to normal antibody response to gI. None of the pigs mounted a secondary neutralizing antibody response to PRV.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
13.
Rijsewijk FA Luiten EJ Daus FJ van der Heijden RW van Oirschot JT 《Veterinary microbiology》1999,65(1):1-7
Canine herpesvirus (CHV1) is found in dogs all over the world and may spread by oronasal or sexual contact. We developed an enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against CHV1 in dogs. The antigen used for this ELISA was prepared by purifying CHV1 virions from the medium of infected A72 cells. To investigate the prevalence of CHV1 in The Netherlands, a panel of 145 sera of dogs boarding at a kennel in Lelystad, The Netherlands, was screened using this ELISA. The dogs originated from all parts of The Netherlands and represented many different breeds. The sera were collected both at the start and at the end of the boarding period. Of the 145 paired sera 61 (42.1%) were positive, 79 (54.5%) were negative and 5 (3.4%) could not be attributed to either group. None of the negative dogs became seropositive during the boarding period, which lasted normally two to three weeks. We also tested 79 individual sera taken from dogs at various other places in The Netherlands and found that 27 (34.2%) were positive. Hence, in total 224 dog sera, collected from April 1997 to March 1998, were tested and 88 (39.3%) were found positive. We conclude that the prevalence of CHV1 seropositive dogs in The Netherlands in this period was about 40%, and that boarding at a dogs kennel did not contribute to the spread of CHV1. In addition, CHV1 has been isolated from two clinical cases of fatal haemorrhagic disease in The Netherlands. 相似文献
14.
Ron Porat Victor Vinokur Batia Weiss Lea Cohen Avinoam Daus Eliezer E. Goldschmidt Samir Droby 《European journal of plant pathology / European Foundation for Plant Pathology》2003,109(9):901-907
-Aminobutyric acid (BABA), an inducer of pathogen resistance in plants, induced disease resistance in reproductive parts of the plant, such as grapefruit peel tissue. Application of BABA to specific wound sites on the fruit peel surface induced resistance to Penicillium digitatum, the main postharvest pathogen of citrus fruit, in a concentration-dependent manner, being most effective at 20mM, and rather less effective at either higher or lower concentrations. The effect of BABA in inducing resistance to P. digitatum in the fruit peel surface was local and limited to the vicinity (within 1–2cm) of the BABA-treated site. In addition to inducing pathogen resistance, increasing concentrations of BABA (from 1 to 100mM) also exhibited direct antifungal activity and inhibited P. digitatum spore germination and germ tube elongation in vitro. The induction of resistance to P. digitatum by BABA was accompanied by the activation of various pathogen defense responses in grapefruit peel tissue, including activation of chitinase gene expression and protein accumulation after 48h, and an increase in phenylalanine ammonia lyase (PAL) activity after 72h. 相似文献