Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

农业中介组织在四川农业信息服务中的作用及其发展方向

本文分析了四川省农业信息服务体系的构成,该体系主要由农业行政管理服务体系、图书情报服务体系和农业中介组织组成;概述了中介组织在四川农业信息服务中的主要作用以及随着市场经济的发展农业中介组织在未来的发展方向。     

基于扩展DEA模型的四川省油菜生产效率研究

为了客观评价四川油菜生产能力、市场竞争力,找到影响油菜生产效率提高的主要因素,运用扩展的DEA模型,对2008—2014 年间四川油菜生产效率进行了实证分析,同时将四川与中国油菜主产区中其他6 个省的生产效率进行了比较分析。结果表明：从整体来看四川油菜生产规模没有发挥到最佳状态,导致综合效率无效,实际单产低于潜在水平;另投入结构失衡程度较为严重,人工成本冗余量大。在省域层面上,湖北省油菜生产各效率较为突出,达到了生产前沿且生产较为稳定,其次是青海、江西,四川处于第四位,在比较区域中只具有比较优势。建议通过合理改善要素投入结构,适度调整生产规模,继续强化技术创新,提高生产机械化水平等途径提高四川油菜生产效率。     

Affinity isolation and mass spectral analysis of 1,10-phenanthroline (OP)-stimulated UV-damaged-DNA binding proteins expressed in zebrafish (Danio rerio) embryos

Our earlier studies indicated the high expression of a UV-damaged-DNA binding activity in zebrafish (Danio rerio) embryos at 12?h postfertilization (hpf). Two 30- to 35-kDa polypeptides homologous to the N-terminal lipovitellin 1 (Lv1) domain of the 150-kDa zebrafish vitellogenin 1 (zfVg1) were identified as the damage recognition factors in zebrafish extracts, and the metal-chelating agent 1,10-phenanthroline (OP) was found to inhibit the embryonic UV-damaged-DNA binding activity. This study further explored the DNA damage-sensing components in 12 hpf zebrafish extracts. UV-damaged-DNA binding proteins were enriched from zebrafish extracts by isoelectrofocusing. Both OP-sensitive and OP-stimulated, UV-damaged-DNA binding activities were detected in fractionated zebrafish extracts. Two-dimensional gel electrophoresis of proteins captured by an immobilized oligonucleotide carrying a UV-induced (6-4)photoproduct (6-4PP) revealed a 25-kDa polypeptide as the major 6-4PP-binding factor in an OP-stimulated fraction. Three 25-kDa factors that bound weakly to 6-4PPs were also isolated. The four polypeptides having pIs between 7.0 and 7.3 were unreactive to an anti-zfVg1 antibody targeting the Lv1 domain. Mass spectral analysis showed the appearance of amino acid sequences LPIIVTTYAK and IPEITMSK in all 25-kDa polypeptides and sequences exactly matching those contained in the four factors exist only in the C-terminal Lv2 domain of zfVg1, reflecting the origination of these factors from enzymatic cleavage of the Lv2 domain at slightly different positions. The OP-stimulated fraction produced a much stronger UV-dependent DNA incision activity in the presence than in the absence of OP, suggesting the association of these factors with DNA damage repair under metal-deficient conditions.     

四川烟叶产业SWOT分析及发展策略研究

为明确四川烟叶产业在炙热化市场竞争中的情况,打造四川烟叶产业的核心竞争力,推进四川烟叶可持续发展,运用SWOT模型经济学方法,从影响竞争力的各主要要素进行分析,剖析四川烟叶产业的竞争力情况。结果表明四川烟叶产业具有自然资源、基础设施、品种与生产力的优势,同时指出四川烟叶存在生产技术落后、烟叶质量欠佳、服务体系不完善等问题。通过构建SWOT矩阵,针对性提出提高四川烟叶产业市场核心竞争力的发展策略,为四川烟叶产业在新一轮的发展中提供参考。从SWOT经济学分析的方法可以看出四川烟叶产业发展基础良好,提高核心竞争力的各项条件具备,且实际操作切实可行。     

桐城市生猪规模化养殖的实践与思考

循环经济是废弃物管理战略转变的需要和产业链的有机延伸,是把“资源一产品一污染排放”的“单向单环式”的线性经济,改造成“资源一产品一再生资源一产品”多向多环式相结合的反馈经济及循环经济综合模式。桐城市始终坚持在大农业生产领域中引入循环经济理念,进而优化农业生态环境,促使农业生产与生态环保相融合,真正实现农业的可持续发展。     

桐城市兴隆生猪养殖专业合作社的调查与思考

桐城市吕亭镇兴隆生猪养殖专业合作社,自2006年8月成立以来,以＂民办、民管、民受益＂为宗旨,坚持走养殖、加工、销售为一体的产业化发展的路子,采取＂合作社＋协会＋农户＂的运营模式并与入社农民签订保底价收购合同,吸引农民入社,将松散的养殖户连接起来,共同抵御市场风险,探索出了一条合作互助,不断发展壮大养猪业的有效途径。合作社现拥有社员138家,养殖三元杂交猪达3万头。有业务往来的50头以上的养猪户1000余家,年收入达3680万元以上,主要分布在吕亭镇、大关镇、孔城镇及周边的枞阳县、庐江县等乡镇。     

基于CNKI的四川省农业科学院论文的文献计量分析

利用CNKI文献计量分析工具和采用文献统计分析方法,分析了1990～2009年四川省农业科学院发表论文的年度分布、作者分布、学科分布、研究层次、作者单位分布、论文项目资助来源,以及文献来源、中文关键词和论文被引频次等几个指标,以期为本院未来科研发展规划提供参考。     

武陵山生态区青花菜漂浮湿润育苗技术懿探讨与应用

青花菜是武陵山生态区主要的反季蔬菜之一。近年来,武陵山片区试验站探索运用漂浮湿润育苗技术,不仅提早了青花菜生产季节,而且培育出的菜苗根系发达、生长整齐、植株健壮;大田定植省工,成活率提高、发病率降低,平均产量大幅提升。青花莱漂浮湿润育苗技术已得到山区菜农的普遍欢迎。     

山区青花菜漂浮湿润育苗技术

介绍了山区青花菜漂浮湿润育苗技术,主要包括环境条件要求、棚池建设、漂浮液制作、基质装盘、浸种催芽、播种、苗期管理、病虫害防控、控水炼苗、常见问题及对策等内容,以期为种植户提供参考。