三七种子的脱水敏感性与膜质过氧化作用

采用常规生理生化方法对脱水处理后三七种子的活力、发芽率、相对电导率、丙二醛含量、抗氧化系统酶活性进行动态测定,研究其种子脱水敏感性及其影响机制。结果表明,三七种子的临界含水量为20%,随着脱水程度的加深,三七种子的活力、发芽率呈下降趋势,相对电导率和MDA含量逐渐增加,而抗氧化系统酶的活性总体呈先增后降的趋势。膜质的过氧化作用和抗氧化系统酶活性的降低是导致三七种子脱水敏感的主要原因。     

Effect of Dietary Supplementation with Hydrolyzed Wheat Gluten on Growth Performance,Cell Immunity and Serum Biochemical Indices of Weaned Piglets （Sus scrofa）

To investigate the effects of dietary supplementation with hydrolyzed wheat gluten (HWG) on growth performance, cell immunity and serum biochemical indices of weaned piglets, 160 crossed (Large White×Landrace) and weaned piglets were randomly divided into 4 treatments with 4 replicates of 10 piglets each. The piglets in each treatment were fed an experimental diet containing either 0 g kg-1 HWG (control group), 30 g kg-1 HWG (3% HWG group), 50 g kg-1 HWG (5% HWG group), or 2.5 g kg-1 glycyl-L-glutamine (0.25% Gly-Gln group). The results showed that the diarrhea rates in 3% HWG and 5% HWG groups were significantly lower than in control group from d 1 to 14 (P＜0.05), while the average daily gain (ADG) in each of two groups was increased (P＞0.05). When compared with control group, dietary supplementation with 3% HWG increased the ratio of CD4+:CD8+ and the ratio of serum albumin and globulin concentrations (A:G) on d 14 and 28, as well as the proliferation of T- and B-lymphocytes (P＞0.05) on d 28. In addition, on d 14 and 28, the A:G ratio in 5% HWG group was significantly higher than in control group (P＜0.05), while the ratio of CD4+:CD8+ increased slightly (P＞0.05). Interestingly, 0.25% Gly-Gln group had higher proportion of CD3+ (P＞0.05) and CD4+ (P＜0.05) on d 14 than control group, but growth performances of 0.25% Gly-Gln group were negatively affected at all experiment stages. These results suggested that HWG might improve the growth performance of piglets by strengthening cell immunity and decreasing the occurrence of diarrhea during the prophase after weaning.     

宁夏干旱区露地礼品西瓜适应性栽培性状的比较

对8个礼品西瓜品种的植物学性状、抗病性、产量及品质等性状进行了比较试验。结果表明:"黑美人"、"新金兰"不仅抗病、丰产,品质较好,而且产量均超过3 800kg/667m2,在宁夏干旱区露地栽培具备推广价值。     

阿克苏地区中龄期苹果树的根系空间分布研究

采用剖面挖掘和分层取样法,利用WinRHIZO Pro2010a根系分析系统对新疆阿克苏地区苹果园漫灌条件下的中龄期(基径9 cm)红富士苹果树的吸收根(根径<2 mm)和输导根(根径≥2 mm)的空间分布状况进行了研究。结果表明,在水平方向的土壤中,苹果树吸收根与输导根根长密度与根表面积表现出"单峰型"的分布规律,吸收根根长密度和根表面积最大值出现在离树干水平距25～50 cm处的土壤内,输导根根长密度和根表面积最大值出现在离树干水平距0～25 cm处的土壤内,80%以上的根系分布在离树干水平距0～125 cm的土壤内。在垂直方向即土层中,苹果树的吸收根根长密度表现出"双峰型"的分布规律,吸收根根表面积与输导根的根长密度和根表面积都表现出"单峰型"的分布规律,80%以上的吸收根与输导根分布在0～80 cm的土层内。总之,距树干0～125 cm的土壤内及0～80 cm的土层是中龄期苹果树的重要分布区域,可作为拟定阿克苏地区苹果园中龄期苹果树田间水分、养分管理措施的理论依据。     

原子力显微镜对TiN薄膜的分析研究

主要论述了一种高精度的原子力显微镜AFM.IPC-208B型机在分子形态学方面的应用研究.以磁控溅射获得的TiN薄膜为例,从原子力显微镜测得的三维图上探析该TiN薄膜的优先生长面及其在优先生长面上的原子排布.实验不仅鉴定了测试材料的微观形态,也充分肯定了该原子力显微镜原子量级的精度及其在微观结构领域的潜在发展,为该机应用于微加工领域奠定了基础.     

我国牛轮状病毒的分离鉴定和疫苗研究进展

约50%的犊牛腹泻是由牛轮状病毒(Bovine Rotavirus, BRV)引起的,它制约着我国养牛业的发展.论文综述了牛轮状病毒毒株的分离、鉴定方法及疫苗研制的现状,并对BRV新型疫苗的发展趋势进行了展望.     

金针菇航天种菌丝诱变效应的研究

本实验以2个金针菇航天诱变菌种(航金1号和航金2号)和地面对照菌种(江山白)为材料,进行了拮抗实验、菌丝生长速度实验、抗杂性实验和酯酶同工酶分析实验研究,结果表明,金针菇航天种与对照之间有拮抗现象,但无拮抗线。航天搭载菌株的菌丝生长速率比对照快,其中航金1号生长速度最快。航金1号和航金2号均表现出较强的抗杂性,能抑制并盖过霉菌菌丝生长。通过酯酶同工酶聚丙烯酰胺凝胶电泳分析发现,航金1号和航金2号与对照相比都出现了特异谱带,说明经过航天诱变的菌株发生了一定的突变。     

Effect of ovariectomy on biological characteristics of MSCs from SD rats

AIM: To investigate the effects of ovariectomy on the biological characteristics of the mesenchymal stem cells (MSCs) derived from the osteoporotic (OP) SD rats and normal rats by comparing the morphology, growth curve and cell cycle. METHODS: Female SD rats of 10-month old were randomly divided into model group and sham operation group (10 rats in each group). The osteoporotic model was established by ovariectomy. Twelve weeks after ovariectomy, the MSCs were isolated from normal and OP rats, and purified by differential time adherent method. The morphological difference of the MSCs between normal and OP rats was observed under inverted phase contrast microscope, transmission electronic microscope (TEM) and scanning electronic microscope (SEM). The growth curves of the MSCs were detected by MTT method. The cell cycles of the MSCs were determined by flow cytometry. RESULTS: Compared to the MSCs from normal rats, the morphology of the MSCs form OP rats became wider and flatter, the cell organelles were decreased and the cell viability was descended. The differentiation of the MSCs from OP rats into adipocytes appeared and the cell multiplication capacity was declined. CONCLUSION: The cell morphology of the MSCs derived from rats with ovariectomy changes obviously and the cell multiplication capacity is decreased.     

甘肃省双孢蘑菇栽培的3种技术模式

甘肃省地处西北高原,地形复杂,气候类型多样,形成了双孢蘑菇栽培模式的多样化。从适宜区域、季节安排、栽培原料、品种选择、栽培设施、栽培特点及安全高效生产的栽培技术要点等方面,介绍了甘肃省双孢蘑菇栽培的3种技术模式,即河西走廊沿山冷凉区夏季反季节栽培技术模式、陇东南地区秋冬栽培技术模式和荒漠绿洲区越冬栽培技术模式。