Preservation of fowl semen in liquid nitrogen: Application to breeding programmes

1. A method of freezing semen of individual males was adapted for use under farm conditions using an automated freezing apparatus.

2. An insemination programme to produce high fertility and hatchability with semen which had been deep frozen for 2 months was devised.

3. Over 90% fertile eggs with a 90% hatch of all eggs set was obtained with frozen and thawed semen over a period from the 2nd to the 12th day after the first of four inseminations. The persistency of fertility was also tested and 93, 86.6 and 30.7% of the eggs were fertile during days 2 to 6, 2 to 8 and 9 to 15 after the last insemination.

4. Corresponding with the high fertility rate, chicks were produced by every hen that was inseminated and from every male whose semen was frozen and stored. The implications for future breeding practices of this successful result are discussed.     

Toxoplasma infection and abortion in dairy goats

Summary Serological investigation by the indirect haemagglutination test forToxoplasma antibodies was carried out in a flock of dairy goats. Antibody titres ranging from 1:4 to 1:1,024 were found in 34% of 371 apparently healthy animals. High antibody titres of 1:256 suggestive of recent infection were noted in 18 (4.9%) animals including 13 nannies. The occurrence of a number of non-brucella abortions associated with high serological titres and the isolation of the parasite from the brain tissues of an aborted foetus suggestedToxoplasma as a probable aetiologic factor. Sera having significant levels of haemagglutinating antibodies (1:128) were also found positive to the card agglutination test. Epidemiological aspects and the public health potential of subclinical toxoplasmosis in dairy goats are discussed.

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Infecciones Y Abortos En Cabras Lecheras Debido AToxoplasma

Resumen Se realizaron investigaciones serológicas de toxoplasmosis mediante la prueba indirecta de hemaglutinación en un rebaño de cabras lecheras. Se encontraron títulos de 1:4 a 1:1024 en 34% de 371 animales aparentemente sanos. Títulos de 1:256 sugestivos de infecciones recientes, se encontraron en 18 (4.9%) animales, incluyendo 13 hembras paridas. La ocurrencia de un significativo número de abortos no asociados con brucela, títulos serológicos altos de toxoplasmosis y el aislamiento del parásito del cerebro de fetos abortados, sugiere que eltoxoplasma es el factor etiológico más importante. Se discuten aspectos epidemiológicos de importantcia para la salud pública, relacionados con el potencial de la toxoplasmosis (subclínica) en cabras lecheras.

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Toxoplasmose Et Avortement Chez Les Chevres Latiteres

Résumé Des investigations sérologiques par la méthode de l'agglutination indirecte pour rechercher des anticorps toxoplasmiques ont été faites sur un troupeau de chèvres laitières. Des anticorps ont été mis en évidence chez 34 p.100 des 371 animaux apparemment sains à des titres allant de 1:4 à 1:1024. Des titres élevés d'anticorps atteignant 1:256 ont été relevés chez 18 (soit 4,9 p.100) des animaux comprenant 13 chèvres, ce qui fait penser à une infection récente.L'existence de nombre d'avortements non-brucelliques associés avec des titres sérologiques élevés et l'isolement du parasite des tittus cérébraux d'un foetus avorté suggerent que les Toxoplasmes en ont probablement été le facteur étiologique. Des sérums ayant des taux significativement élevés d'anticorps agglutinants (1:128) ont également été reconnus comme positifs par le test d'agglutination en EAT. Les aspects épidémiologiques et le danger potentiel pour l'homme de ces cas subcliniques de toxoplasmose chez les chèvres laitières sont discutés.

     

Feather damage in hens caged individually

1. The plumage of hens housed singly in cages was generally in good condition after one year. There was slightly more feather loss where hens could peck their neighbours’ plumage (adjacent cages) than where this was not possible (spaced cages).

2. It was concluded that most, though not all, of the feather damage seen in the individually‐caged birds was due to abrasion, but that abrasion was likely to be relatively unimportant in multi‐bird cages.

3. There were no significant differences in egg production or food intake associated with the two kinds of housing.

4. In the adjacent cages there was an increase in maintenance requirement related to increased feather damage, which amounted to about 7% for an increase of about one unit in feather‐loss score. In the spaced cages the increase was less.

5. Hens with increased feather damage also showed increased egg production.     

Evaluation of rabbit excreta as an ingredient in broiler diets

1. Dry rabbit excreta were found to contain 188.1 g crude protein, 6.6 g non‐protein nitrogen, 0.3 g ammonia, 89.8 g moisture, 8.0 g ether extractives, 135.2 g crude fibre, 266.9 g ash and 19.18 MJ gross energy/kg. Methionine and lysine contents were 3.95 and 4.29 g/100 g protein, respectively.

2. In one experiment, 0, 50, 100 or 200 g rabbit excreta/kg were substituted for maize in a broiler diet. Chickens receiving the diet containing 200 g rabbit excreta/kg gained significantly less weight to 8 weeks of age than those receiving other diets.

3. In a second experiment, 0, 100, 150 or 200 g rabbit excreta/kg were substituted primarily for maize in a broiler diet to provide equal energy and protein contents. Weight gains to 8 weeks of birds receiving these diets did not differ significantly. The uncorrected metabolisable energy content of the excreta was 9.15 MJ/kg.

4. There were no gross pathological changes in the birds fed on the experimental diets.     

Physiological actions of dinoterb,a phenol derivative: 2. Effects on isolated plant mitochondria and chloroplasts

Dinoterb, a contact herbicide, affects respiration and photosynthesis of mitochondria and chloroplasts. On mitochondria, at low concentrations, it acts as an uncoupler of oxidative phosphorylation; at higher concentrations, it inhibits the electron transport chains, probably before cytochrome c. On chloroplasts, dinoterb has a stimulatory effect on oxygen uptake in the reduced dichlorophenol-indophenol→methyl viologen couple; however, it is also an inhibitor of the Hill reaction and its site of inhibition is located before plastoquinone, near photosystem II.     

Effect of the proteinase inhibitor aprotinin in the management of hemorrhagic shock in the dog.

Aprotinin, a proteinase inhibitor, was evaluated as a pharmacologic aid in dogs subjected to lethal hemorrhagic shock. Survival time, hemodynamic changes, and plasma enzyme analysis were measured as criteria for drug effects. Mixed-breed dogs (n = 14) were divided into 2 groups of 7 each: nontreated dogs in shock (group 1) and aprotinin-treated dogs in shock (group 2). One of 7 dogs in group 1 and 2 of 7 dogs in group 2 survived. Survival time, for the remaining dogs in group 1 (190 min, n = 6) and group 2 (188 min, n = 5) were not significantly different. There was no significant difference in mean arterial pressure, mean pulmonary arterial pressure, cardiac output, or left ventricle systolic pressure associated with aprotinin treatment at any time after hemorrhagic shock. There was no significant difference in plasma lactic acid, aspartate aminotransferase, alanine aminotransferase, creatine phosphokinase, alpha-amylase, and beta-glucuronidase associated with treatment at any time; however, there were significant (P less than 0.05) increases with time. The gastrointestinal tract was the site of most obvious lesions found at necropsy. Lesions varied considerably in extent and severity without apparent correlation to the treatment regimen. These experiments did not show beneficial effects of aprotinin in dogs subjected to hemorrhagic shock, but neither did they completely rule out some valuable actions that may have been obscured by the type of model used.     

The effects of low voltage current on healing of thermal third degree wounds.

Bilateral third degree burns were created on the flanks of eight miniature swine. Each animal had one side treated daily with low intensity direct (LIDC) of 400 microamperes (muA) by contact electrode for 2 hours morning and afternoon until healing occurred. The polarity of the electrode was negative in 4 pigs and positive in 4 pigs. One group of 4 pigs had the eschar surgically removed 24 hours post burn. Weekly biopsies and cultures were taken of treated and control wounds. Results indicated: local treatment of 400 muA LIDC did not hasten or delay healing of thermal third degree burns; escharectomy hastened wound healing by 10 days, and superficial proteus infection occurred in 80% of all wounds.     

Kinetics of NDV specific antibodies in chickens—V. Analysis of frequency distributions of antibody titers against Newcastle disease virus by investigation of random samples in chicken flocks

Chicks and chickens maintained under commercial conditions were vaccinated against Newcastle Disease via drinking water. Prior and after different times of vaccination blood samples were drawn from different numbers of birds and checked for HI antibodies. The modes of distribution of the antibody titers within the random sample were assayed. The following results were obtained:

1. 1. On the basis of the distribution of the serum titers can be concluded whether the antibody level within a flock is increasing or decreasing.

◦ —A right steep asymmetry can be observed up to 20 days post vaccination.

◦ —In the phase of maximal antibody levels an almost symmetric distribution of the titers is present.

◦ —In later times (more than three weeks p. vacc.) the distribution shows a left steep asymmetry (Poisson distribution).

2. 2. A poisson distribution is also observable during the elimination of maternal antibodies of chicks until complete elimination.

3. 3. The mode of distribution of the HI titers in sera of day-old chicks correlates with the mode of distribution of the dams. Therefore, conclusions are possible from the status of the chicks to the dams and reverse.

4. 4. Factors which interfere with the mode of distribution are:

◦ —Two or more peaks after vaccination of chickens. This indicates an uneven immune response within the flock.

◦ —Distributions with several peaks may also occur if flocks are composed of day-old chicks from parent flocks with different levels of antibody titers.