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81.
The effect of serum from horn cancer affected bullocks and cows on E-rosetting capacity of peripheral blood lymphocytes (PBL) from unaffected control animals was examined. The E-rosette counts were made using 2-aminoethyl isothiouronium bromide (AET) treated sheep red blood cells. A significant decrease in the percentage of EAET rosette forming cells was noticed when PBL were incubated with 50 per cent serum from animals affected with horn cancer. However, no such effect was noticed when PBL were treated with 50 per cent serum from unaffected control animals. A linear relationship was observed between percentage of EAET rosette forming cells of animals affected with horn cancer and E-rosette inhibitory activity of the corresponding serum on PBL from control animals. 相似文献
82.
83.
Twenty-seven insecticides, including organochlorine, organophosphorus compounds and carbamates were tried for potentiation of the toxic effect of malathion. Carbaryl, diazinon, fenthion, gamma-BHC, parathion and tetrachlorvinphos potentiated the action of malathion. Fourteen insecticides gave additive effects while seven proved antagonistic. Insecticides which showed potentiation, were further tested by mixing with malathion in the ratios of 1:9, 3:7, 5:5, 7:3 and 9:1. All the five combinations in the six mixtures gave potentiating effects. Maximum potentiation of 17.6 times was observed in 1:9 combination of malathion and gamma-BHC. The proportions in which the insecticides were mixed had a pronounced effect on the toxic effect of malathion. 相似文献
84.
Sharma AK Kumar N Dimri U Hoque M Maiti SK Gupta OP Shahi A 《Journal of veterinary medicine. A, Physiology, pathology, clinical medicine》2004,51(9-10):420-424
The study was conducted on 10 buffalo calves with a weight of 98.5 +/- 3.9 kg and age 9.7 +/- 1.3 months. Ten trials of two treatments were carried out using a randomized block design. Atropine at the dose of 0.02 mg/kg bodyweight was administered in both the groups. The animals of group I received romifidine at the dose of 10 microg/kg i.v., 10 min after atropine administration, whereas, animals of group II received triflupromazine at the dose of 0.3 mg/kg i.m. and 10 min later romifidine at the dose of 10 microg/kg i.v. immediately followed by ketamine at the dose of 5 mg/kg i.v. The onset of action of romifidine in group I occurred within 2 min and the animals remained under mild sedation for 31 +/- 4.8 min. In group II, the triflupromazine-romifidine-ketamine combination induced anaesthesia for 14 +/- 2.3 min. Hypothermia, significant bradycardia and respiratory depression was noticed in both groups at different time intervals. 相似文献
85.
86.
Association mapping was undertaken in common wheat to identify markers associated with pre-harvest sprouting tolerance (PHST). For this purpose, a population of 242 wheat genotypes and 250 SSR markers were used. The population used consisted of diverse germplasm, which carried sufficient phenotypic variation for PHS for conducting association mapping. The population was found to be structured and stratified into 15 sub-populations; the tolerant and moderately tolerant wheat genotypes were distributed in all the sub-populations. This feature of the population along with other information on population structure was used in association mapping using both the available models, the general linear model (GLM) and the mixed linear model (MLM); hopefully, this minimized the rate of false positives. As many as 30 markers were found to be associated with PHST, 26 markers with GLM and 17 markers with MLM; 13 markers were detected using both the approaches. Only eight SSR markers associated with QTL for PHST were such, which were located within the marker intervals that were earlier reported to carry QTLs for PHST. The remaining 22 markers that were found to be associated with PHST could not be associated with any of the genomic regions known to carry QTLs for PHST, which are known to occur on all the 42 chromosome arms of wheat genome. 相似文献
87.
Gupta S Khanna VK Maurya A Bawankule DU Shukla RK Pal A Srivastava SK 《Fitoterapia》2012,83(6):1092-1099
This study was undertaken to ascertain the antipsychotic properties of Rauwolfia tetraphylla L. leaves and to isolate and characterize the antipsychotic constituents. Among the MeOH extract and some alkaloidal fractions at different pHs, the alkaloidal CHCl(3) fraction at pH-9 (2C) showed the highest antipsychotic activity against dopaminergic (DA-D(2)) and serotonergic (5-HT(2A)) receptors in-vitro and amphetamine induced hyperactive mouse model in-vivo. The activity guided isolation of CHCl(3) fraction (2C) afforded six indole alkaloids: 10-methoxytetrahydroalstonine (1), isoreserpiline (2), an isomeric mixture of 11-demethoxyreserpiline (3) and 10-demethoxyreserpiline (4), α-yohimbine (5) and reserpiline (6). Given orally, alkaloids 3-6 showed significant antipsychotic activity in a dose dependent manner. None of the extract, alkaloidal fractions or alkaloids showed any extra pyramidal symptoms at the tested doses. It was also observed that MeOH extract was behaving similar to other clinically used novel atypical antipsychotics in having 5-HT(2A) occupancy greater than the DA-D(2) receptor at the tested doses. Further toxicity and safety evaluation studies of MeOH extracts of R. tetraphylla leaves at different doses (10, 100, 300 and 2000 mg/kg) on female Swiss albino mice showed that MeOH extract is non toxic. The isolated alkaloids, 3-6 could serve as a promising lead structure for drug development of treating psychotic conditions in human. 相似文献
88.
Gupta RC Canerdy TD Lindley J Konemann M Minniear J Carroll BA Hendrick C Goad JT Rohde K Doss R Bagchi M Bagchi D 《Journal of animal physiology and animal nutrition》2012,96(5):770-777
The investigation was conducted on client-owned moderately arthritic dogs with two objectives: (i) to evaluate therapeutic efficacy of type-II collagen (UC-II) alone or in combination with glucosamine hydrochloride (GLU) and chondroitin sulphate (CHO), and (ii) to determine their tolerability and safety. Dogs in four groups (n = 7-10), were treated daily for a period of 150 days with placebo (Group-I), 10 mg active UC-II (Group-II), 2000 mg GLU + 1600 mg CHO (Group-III), and UC-II + GLU + CHO (Group-IV). On a monthly basis, dogs were evaluated for observational pain (overall pain, pain upon limb manipulation, and pain after physical exertion) using different numeric scales. Pain level was also measured objectively using piezoelectric sensor-based GFP for peak vertical force and impulse area. Dogs were also examined every month for physical, hepatic (ALP, ALT and bilirubin) and renal (BUN and creatinine) functions. Based on observations, significant (p < 0.05) reduction in pain was noted in Group-II, III, and IV dogs. Using GFP, significant increases in peak vertical force (N/kg body wt) and impulse area (N s/kg body wt), indicative of a decrease in arthritis associated pain, were observed in Group-II dogs only. None of the dogs in any group showed changes in physical, hepatic or renal functions. In conclusion, based on GFP data, moderately arthritic dogs treated with UC-II (10 mg) showed a marked reduction in arthritic pain with maximum improvement by day 150. UC-II, GLU and CHO operate through different mechanisms of action, and were well tolerated over a period of 150 days. 相似文献
89.
Das A Katole S Kumar A Gupta SP Saini M Swarup D 《Journal of animal physiology and animal nutrition》2012,96(3):442-449
A feeding trial was conducted to determine the optimum level of crude protein (CP) in the diet of captive blackbuck (Antelope cervicapra) in which feed consumption and nutrient utilization are maximal. Fifteen blackbucks (BW 25-34 kg) were distributed into three groups of five each in an experiment of 75-days duration including a digestion trial of 5-day collection period. All the animals were offered 200 g of concentrates and fresh maize fodder ad libitum. The overall CP content of the three respective diets was 6.9%, 10.4% and 12.7%. Blood samples were collected on the last day of the experiment. Intake and digestibility of CP increased (p < 0.01) with the increased level of CP in the diet. Feed consumption and nutrient intake were not significantly different among the groups. However, digestibilities of most of the nutrients were higher in the 10.4% CP diet than in the 6.9% CP diet. The endogenous loss of nitrogen was similar among the groups. Based on the endogenous losses, minimum N requirement was calculated to be 776 mg/kg BW(0.75) /day, and to meet this requirement, diet must contain at least 8.27% CP. Serum urea nitrogen concentration increased (p < 0.01) with increased level of dietary CP. Serum level of glutamate oxaloacetate transaminase and glutamate pyruvate transaminase was higher (p < 0.05) in the group fed 6.9% CP diet. Animals in the group fed low protein diet also lost body mass during the experimental period. It was concluded that a diet containing 10.4% CP was optimum for maximizing nutrient utilization without any adverse effect on voluntary feed consumption and serum metabolite profile of blackbucks. 相似文献
90.
Kazmi I Rahman M Afzal M Gupta G Saleem S Afzal O Shaharyar MA Nautiyal U Ahmed S Anwar F 《Fitoterapia》2012,83(1):142-146
A new stearoyl glucoside of ursolic acid, urs-12-en-3β-ol-28-oic acid 3β-d-glucopyranosyl-4′-octadecanoate and other compounds were isolated from the leaves of Lantana camara L. The structure of this new glycoside was elucidated and established by standard spectroscopic methods. In streptozotocin-induced diabetic rats it showed significant reduction in blood glucose level. 相似文献