不同肉鸡用植物性饲料总磷真利用率的测定

本试验利用无磷原料配制无磷饲粮,选择低磷原料配制基础饲粮,用一定比例待测植物性饲料替代基础饲粮配制待测饲粮,通过平衡试验法测定了35日龄艾维茵肉仔鸡对玉米、豆粕、麦麸3种饲料原料的总磷真利用率。结果表明:在控制饲粮含磷量低于肉仔鸡的磷需要量,研究饲料磷的最大利用率时,肉仔鸡的内源磷排出量为(44.84±3.14)mg/d。玉米、豆粕和麦麸的总磷真利用率分别为(16.42±3.36)%、(28.34±4.90)%和(46.48±4.29)%。利用总磷含量的1/3和非植酸磷含量估计肉鸡饲料有效磷并不准确。因麦麸本身含有高活性的植酸酶,肉仔鸡可部分利用其植酸磷。     

天祝县草产业发展的制约因素及其对策

通过分析天祝县草产业发展现状，指出天祝县草产业发展存在的问题，提出要大力发展天祝县草产业必须加强天然草原综合改良，科学合理利用草原资源，建立人工草地和牧草种子繁育基地，开发草产品；建立多元化的投入机制，培植草产业开发的龙头企业；进一步完善草地管理制度，全面彻底地落实草原有偿承包责任制；建立信息网络体系，把信息网络纳入畜牧业基础设施建设范围内，建立健全草畜产品信息网络。     

猪白细胞介素-18基因表达及重组蛋白的纯化

以pcDNA3.1-IL18为模板进行PCR扩增获得猪白细胞介素-18(IL-18)的成熟肽基因,以KpnⅠ、SacⅠ双酶切PCR产物作为供体,KpnⅠ、SacⅠ双酶切的pET41c和pET32c作为载体,连接载体供体,转化DH5α,经双酶切、PCR鉴定及测序筛选出阳性重组质粒,并命名为pET41c-IL18和pET32c-IL18。将pET41c-IL18和pET32c-IL18转化大肠埃希氏菌BL21(DE3),用1 mmol/L IPTG 37℃诱导表达。SDS-PAGE及W estern-blotting分析结果表明,所表达的重组蛋白主要以包涵体形式存在,pET32c-IL18重组蛋白分子质量约33 ku,与预期大小相符。将包涵体提取物用8 mol/L脲变性,经N i-NTA柱纯化,透析复性,得到了纯化的IL-18蛋白。     

中秋蚕农药污染的生物试验

2006年中秋蚕收蚁后的1-2d内。桐乡市和海宁市相继出现了大面积家蚕中毒，这次中毒发生时间早、范围广、损失蚕种多。两市中秋蚕于9月1-3日收蚁．收蚁当天中毒程度均较轻，至次日，随着食桑次数的增加，中毒加重甚至死亡；发现中毒早，能够及时改用无毒桑叶的，后期饲养基本正常。     

牦牛输卵管上皮细胞和卵泡颗粒细胞的培养

分离培养牦牛输卵管上皮细胞和卵泡颗粒细胞的目的是克服体外受精中早期胚胎发育阻断,建立有利于牦牛早期胚胎体外发育的共培养体系.采集牦牛输卵管上皮细胞进行原代及传代培养,同时从卵泡液中获取颗粒细胞进行原代培养,培养过程中观察2种细胞的生长方式和形态特点.输卵管上皮细胞贴壁生长时,呈多边形,且呈单层成簇生长.培养144 h～216 h,可形成细胞单层.颗粒细胞贴壁生长时,呈现聚集生长特性,贴壁细胞形状不规则,呈放射状.培养72 h～96 h,形成颗粒细胞单层.     

Biological characteristics of JAK2 transduced CD34+ cells from cord blood during ex vivo expansion

AIM: To explore the feasibility and biological characterization of long-term regulated expansion of JAK2 transduced human CD34⁺ cord blood cells in vitro.METHODS: A retrovirus (RV) vector which contains JAK2 catalytic domain and two binding sites for a chemical inducer,dimerization (AP20187),was cloned (designated MGI-F₂JAK2).CD34⁺cells were enriched from cord blood with a MiniMACS system.The purified CD34⁺ cells were transfected with supernatant from the retrovirus packaging cell line that expressed JAK2.Following transduction,cells were expanded into four groups: AP20187 alone,FL alone,TPO,alone,AP20187+FL+TPO,respectively.The expanded cells were monitored by GFP expression,immunophenotyping,progenitor colony assay,karyotype analysis as well as tumorigenesis in nude mice.RESULTS: The purity of selected CD34⁺ cells was over 91% and gene transfer rate was 49.32%±6.21%.Only the group of AP20187 +FL+ TPO was obtained a significant sustained outgrowth of the transduced CD34⁺ cord blood cells.The percentage of GFP+ cells consistently produced a rise to the 90% peak level by the end of 8th week of culture.Flow cytometry analysis showed that the phenotype of the expanded cells was CD33⁺,CD61⁺ and Gly-A⁺ partial positive;CD38⁺ and HLA-DR⁺ strong positive,while CD2,CD7 and CD19 were almost negative.Colony assays performed in methycelluos,which can give rise to BFU-E,CFU-GM and CFU-Mix,the CFU-GM was predominantly in all colonies.The tumor was not observed in nude mice and the karyotype analysis was normal from expanded cells.CONCLUSION: The results demonstrate that AP20187-mediated activation of JAK2 signaling is capable of stimulating expansion JAK2 transduced CB CD34⁺ cells in combination with FL and TPO.This system may have applications for studies in signaling transduction,hematopoiesis,and for gene and cell therapy.     

渠道渐变体土方计算的精确公式

渐变体作为衔接不同断面形式的过渡段,被广泛地用于输水建筑物工程中,但其土方计算目前没有统一科学的计算方法.为此,本文列出了工程中常见的几种渐变段形式,并通过对断面函数数学特性进行分析,得妹到了常见渐变体任一断面面积函教为二次抛物线.因此,可通过起始断面面积,终止断面面积和中断面面积唯一确定任一断面面积函数,并将该函数沿渐变体长度方向积分,推导出渐变段土方计算的精确、通用计算公式.计算实例表明计算过程简单、误差小,概念清晰,可供设计人员参考.     

柴油机喷雾液滴碰撞模型的改进研究

针对用KIVA-3V软件模拟液滴碰撞的O’Rourke模型所存在的网格敏感性问题,将Nordin的MIC(Mesh Independent Collision)模型应用于液滴碰撞模拟,采用定容室对MIC模型和O’Rourke模型在不同网格喷油位置、网格密度和油滴个数条件下的喷雾形状和液滴平均半径进行了模拟比较。比较结果显示,MIC模型的稳定性、精度和可靠性等均比O’Rourke模型要好。     

Correlation of gene modification between histone H3 lysine 4 trimethylation and DNA methylation in IgA nephropathy patients

AIM: To investigate the correlation of gene modification between histone H3 lysine 4 (H3K4) trimethylation and DNA methylation in IgA nephropathy patients. METHODS: H3K4 trimethylation variations were analyzed in peripheral blood mononuclear cells (PBMCs) from 40 IgAN patients and 40 healthy controls by the method of chromatin immunoprecipitation linked to microarrays (ChIP-chip). ChIP real-time PCR was used to validate the microarray results. Quantitative real-time PCR (qRT-PCR) was employed to analyze mRNA expression. DNA methylation in 4 selected positive genes was analyzed by the method of methylated DNA immunoprecipitation-quantitative PCR (MeDIP-qPCR). RESULTS: IgAN patients displayed higher H3K4 trimethylation level and lower DNA methylation level than those of the healthy controls. There were significant differences in DNA methylation and H3K4 trimethylation of 4 selected genes between IgAN patients and the healthy controls (P<0.05). CONCLUSION: Our studies indicate that significant alterations of H3K4 trimethylation and DNA methylation exist in IgAN patients. There is a correlation of gene modification between DNA mathylation and histone H3 lysine 4 trimethylation in IgAN patients.     

饲用植物的民族植物学--内蒙古阿鲁科尔沁旗的研究案例

论述了原住民利用饲用植物资源的传统知识和经验的民族植物学研究价值，提出了我国饲用植物资源的民族植物学研究的主要内容，并以内蒙古阿鲁科尔沁旗的研究结果作了案例进行了深入的探讨。