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981.
Rehtanz M Bossart GD Doescher B Rector A Van Ranst M Fair PA Jenson AB Ghim SJ 《Veterinary microbiology》2009,133(1-2):43-53
Papillomaviruses (PVs) have been shown as being the etiologic agents of various benign and malignant tumours in many vertebrate species. In dolphins and porpoises, a high prevalence of orogenital tumours has recently been documented with at least four distinct novel species-specific PV types detected in such lesions. Therefore, we generated the immunological reagents to establish a serological screening test to determine the prevalence of PV infection in Atlantic bottlenose dolphins [(Tursiops truncatus (Tt)]. Using the baculovirus expression system, virus-like particles (VLPs) derived from the L1 proteins of two TtPV types, TtPV1 and TtPV2, were generated. Polyclonal antibodies against TtPV VLPs were produced in rabbits and their specificity for the VLPs was confirmed. Electron microscopy and enzyme-linked immunosorbent assay (ELISA) studies revealed that the generated VLPs self-assembled into particles presenting conformational immunodominant epitopes. As such, these particles are potential antigen candidates for a TtPV vaccine. Subsequently, the VLPs served as antigens in initial ELISA tests using sera from six bottlenose dolphins to investigate PV antibody presence. Three of these sera were derived from dolphins with genital tumour history and showed positive PV ELISA reactivity, while the remaining sera from lesion-free dolphins were PV antibody-negative. The results suggest that the developed screening test may serve as a potential tool for determining PV prevalence and thus for observing transmission rates in dolphin populations as the significance of PV infection in cetaceans starts to unfold. 相似文献
982.
983.
The average area of agricultural fields in Flanders (Belgium) is about 1.7 ha, being very small compared to fields where precision agriculture is currently applied. Therefore this paper addresses the question whether the within-field variation of soil properties in such fields is structured enough to motivate precision agriculture. To answer this question, 9 soil properties determined on 380 soil samples located in 77 agricultural fields situated in the 5 most dominant pedoscapes of Flanders were used to analyze their spatial variation over intervals ranging from 5 to 900 m. The data set was subjected to a principal component analysis which identified two principal components (PCs) explaining more than 78% of the total variance. The first PC represented the chemical soil properties and the second the physical and biological properties. A variogram analysis of the scores on these two PCs showed that the micro-scale and random variation dominated (82%) the within-field variability of the first PC. The within-field variability of the second PC was dominantly spatially structured (only 37% micro-scale and random variation). Therefore, it was concluded that mainly for soil physical and biological properties (like soil textural fractions and organic matter), the average within-field variation in the small fields of the investigated landscapes is structured enough to allow precision agriculture. 相似文献
984.
At synapses between cortical pyramidal neurons and principal striatal medium spiny neurons (MSNs), postsynaptic D1 and D2 dopamine (DA) receptors are postulated to be necessary for the induction of long-term potentiation and depression, respectively-forms of plasticity thought to underlie associative learning. Because these receptors are restricted to two distinct MSN populations, this postulate demands that synaptic plasticity be unidirectional in each cell type. Using brain slices from DA receptor transgenic mice, we show that this is not the case. Rather, DA plays complementary roles in these two types of MSN to ensure that synaptic plasticity is bidirectional and Hebbian. In models of Parkinson's disease, this system is thrown out of balance, leading to unidirectional changes in plasticity that could underlie network pathology and symptoms. 相似文献
985.
Regulation of the germinal center response by microRNA-155 总被引:1,自引:0,他引:1
Thai TH Calado DP Casola S Ansel KM Xiao C Xue Y Murphy A Frendewey D Valenzuela D Kutok JL Schmidt-Supprian M Rajewsky N Yancopoulos G Rao A Rajewsky K 《Science (New York, N.Y.)》2007,316(5824):604-608
MicroRNAs are small RNA species involved in biological control at multiple levels. Using genetic deletion and transgenic approaches, we show that the evolutionarily conserved microRNA-155 (miR-155) has an important role in the mammalian immune system, specifically in regulating T helper cell differentiation and the germinal center reaction to produce an optimal T cell-dependent antibody response. miR-155 exerts this control, at least in part, by regulating cytokine production. These results also suggest that individual microRNAs can exert critical control over mammalian differentiation processes in vivo. 相似文献
986.
Kirschner M 《Science (New York, N.Y.)》2008,319(5867):1199
987.
Buesseler KO Lamborg CH Boyd PW Lam PJ Trull TW Bidigare RR Bishop JK Casciotti KL Dehairs F Elskens M Honda M Karl DM Siegel DA Silver MW Steinberg DK Valdes J Van Mooy B Wilson S 《Science (New York, N.Y.)》2007,316(5824):567-570
The oceanic biological pump drives sequestration of carbon dioxide in the deep sea via sinking particles. Rapid biological consumption and remineralization of carbon in the "twilight zone" (depths between the euphotic zone and 1000 meters) reduce the efficiency of sequestration. By using neutrally buoyant sediment traps to sample this chronically understudied realm, we measured a transfer efficiency of sinking particulate organic carbon between 150 and 500 meters of 20 and 50% at two contrasting sites. This large variability in transfer efficiency is poorly represented in biogeochemical models. If applied globally, this is equivalent to a difference in carbon sequestration of more than 3 petagrams of carbon per year. 相似文献
988.
989.
It is essential to keep track of the movements we make, and one way to do that is to monitor correlates, or corollary discharges, of neuronal movement commands. We hypothesized that a previously identified pathway from brainstem to frontal cortex might carry corollary discharge signals. We found that neuronal activity in this pathway encodes upcoming eye movements and that inactivating the pathway impairs sequential eye movements consistent with loss of corollary discharge without affecting single eye movements. These results identify a pathway in the brain of the primate Macaca mulatta that conveys corollary discharge signals. 相似文献
990.