The hydrophobic properties of bark extractives

Summary The hydrophobic properties of benzene-soluble extractives from the bark of Pinus radiata were studied by determining the water-solid-air contact angle and the resistance to water penetration.The material contained in the bark extract possessed wax-like and film-forming properties. It was highly hydrophobic with a contact angle with water of about 98°. Wood samples were impregnated with bark extract in order to study the ability of the hydrophobic bark extractives to prevent preferential wetting with water and the displacement of the impregnant from the hydrophilic wood surface. The wax-like material deposited from the bark extracts showed a remarkable affinity for wood as revealed by the high degree of resistance to water penetration and wetting of the treated wood even after several wetting-drying cycles which otherwise tend to break down the bonds and adhesion between a hydrophilic solid and a hydrophobic coating.Hydrophobic or film-forming additives did not improve the ability of bark extractives to protect a hydrophilic solid from water. The properties of the bark extractives as a water repellent and a water barrier could therefore be assumed to be as near perfect as possible.Nature seems to have solved the problem of producing one single, although complex, material with two properties which are extremely difficult, maybe impossible, to combine in a single synthetic material: one of high affinity for a hydrophilic solid and another of extreme water repellency and resistance to water.The in situ conditions prevailing in the bark tissue, combining bark extractives and cell walls of various anatomical structures, must therefore represent a rather unique and for this specific purpose highly efficient two-phase hydrophobic-hydrophilic solid system.The authors would like to express their appreciation to Kalley Timber Development Corporation for the close co-operation and support for our research on bark products and would also like to thank Lewis and Everitt, Poly-Resin Products Ltd., Taeuber and Corssen, Farbenfabriken Bayer AG, Scado-Archer-Daniels, Triton Chemicals and Rohm and Haas for supplying samples and information.     

Stomatal behavior of four woody species in relation to leaf-specific hydraulic conductance and threshold water potential

Midday stomatal closure is mediated by the availability of water in the soil, leaf and atmosphere, but the response to these environmental and internal variables is highly species specific. We tested the hypothesis that species differences in stomatal response to humidity and soil water availability can be explained by two parameters: leaf-specific hydraulic conductance (K(L)) and a threshold leaf water potential (Psi(threshold)). We used a combination of original and published data to estimate characteristic values of K(L) and Psi(threshold) for four common tree species that have distinctly different stomatal behaviors: black cottonwood (Populus trichocarpa Torr. & Gray.), Douglas-fir (Pseudotsuga menziesii (Mirb.) Franco), red alder (Alnus rubra Bong.) and western hemlock (Tsuga heterophylla (Raf.) Sarg.). We used the values to parameterize a simple, nonelastic model that predicts stomatal conductance by linking hydraulic flux to transpirational flux and maintaining Psi(leaf) above Psi(threshold). The model successfully predicted fundamental features of stomatal behavior that have been reported in the literature for these species. We conclude that much of the variation among the species in stomatal response to soil and atmospheric water deficits can be explained by K(L) and Psi(threshold). The relationship between Psi(threshold) and xylem vulnerability to cavitation differed among these species.     

Tree establishment and growth using forage ground covers in an alley-cropped system in Midwestern USA

The integration of forage crops in an alley-cropped system was examined as a method of encouraging tree planting to increase farm income, improve soil quality, and enhance biodiversity on Midwestern U.S. farms. Crop and tree performance were evaluated in an alley-cropped system using four forage intercrops grown in tree alleyways to simulate a potential hay crop – oat (Avena sativa L.) and red clover (Trifolium pratense L.); oat, red clover, and red fescue (Festuca rubra L.); oat, red clover, and orchardgrass (Dactylis glomerata L.); and oat and hairy vetch (Vicia villosa Roth) – compared to a herbicide, mowing and control (no management) treatment. Five tree species, divided into fast-growing hardwoods of two poplar (Populus spp.) clones [Crandon (P. alba L. × P. grandidentata Michx.) and Eugenei (P. deltoids Bartr. × P. nigra L.)], and silver maple (Acer saccharinum L.) were compared with two high-value, slow-growing hardwoods planted from seed and as seedlings: red oak (Quercus rubra L.) and black walnut (Juglans nigra L.). Tree survival in the first year was greater for the fast-growing species, and by the fourth year, tree height among the four forage treatments was equivalent for all upland locations. The oat/hairy vetch ground cover was associated with the shortest trees in the bottomland site. Herbicide-treated trees were taller than the average of the four forage treatments for all four planting conditions. Tree height in the mowing and the control treatments was not significantly different for all four planting conditions. The nutritive value of the forage crop was excellent in the second year of tree establishment, with crude protein content and digestibility at 17 and 71%, respectively, in the oat/red clover/red fescue treatment, suggesting the viability of forage crops as alternatives to herbicides in alley-cropped systems.     

Optimization of an in vitro assay to detect Streptococcus equi subsp. equi

Streptococcus equi is the etiologic agent of a highly infectious upper respiratory disease of horses known as strangles. Bacterial culture methods and polymerase chain reaction (PCR) of nasopharyngeal washes and guttural pouch lavages are used routinely to test clinical and carrier animals for the presence of S. equi but no definitive or gold standard test method has been shown to be optimal. We hypothesized that (i) a flocked swab submerged in ten-fold serial dilution suspensions of S. equi prepared in 0.9% NaCl would detect more colony forming units (CFU) than a rayon swab when used to inoculate a blood agar plate, (ii) centrifugation of a 1ml aliquot of each suspension would improve the limit of detection (LOD) by bacterial culture and PCR compared to the culture or PCR of submerged swab samples, (iii) PCR of the centrifuged samples from each suspension would be more sensitive than aerobic culture alone, and (iv) PCR of a 1ml aliquot directly from a sample would be more sensitive than PCR of a sample following submersion of a flocked swab in 1ml saline. Using 7 ten-fold serial dilutions of S. equi in 0.9% NaCl, the LOD for 4 bacterial culture methods and 3 PCR methods were compared. The LOD of direct PCR and flocked swab culture was determined at 1cfu/ml. All PCR methods were equivalent to each other and were more sensitive than any of the culture methods at the lower dilutions. At higher cell densities (>100cfu/ml) flocked swab culture was not statistically better than rayon swab culture, but it was superior to all other methods tested.     

Effective Evaluation of Equine Extension Programs

Evaluation has become a more significant component of planning and delivering extension programs, as federal partners and granting agencies are requesting information on program and integrated grant outcomes, including participant learning gains, behavior change, and program-generated impacts. Effective evaluation of equine extension programs involves a balance between asking enough well-designed questions to obtain desired information and keeping the evaluation tool brief enough to encourage participant completion. For most faculties, the difficulty with evaluation lies in developing appropriate and useful questions. The objective of this article was to share examples of questions successfully used to evaluate six key equine extension program areas: participant demographics, program logistics, participant behavior change, participant knowledge gain, teaching effectiveness, and program impact. Data generated by postprogram evaluations can be a source of statistically sound information that can be shared with administration, stakeholders, and granting agencies. Extension personnel can use evaluation data to improve planning and delivery of extension programs and to demonstrate teaching ability and program impacts.     

Development and validation of a SYBR green real-time PCR for the quantification of porcine circovirus type 2 in serum, buffy coat, feces, and multiple tissues

The emergence of multiple genotypes of PCV2, as demonstrated by phylogenetic analysis of whole genome or capsid sequences, makes it necessary to have quantitative diagnostic assays that perform equally well on all strains. The objectives of this study were to develop and validate a novel real-time polymerase chain reaction (PCR) assay targeting the highly conserved rep gene (ORF1) and investigate the effects of diagnostic specimen choice on its performance. The assay was tested in naturally infected conventional pigs, experimentally infected gnotobiotic pigs, and plasmid-spiked negative serum, lung tissue, and feces and found to have a linear detection range of 2.2x10(3) to 2.2x10(10) copies of PCV2 per mL. The assay successfully detected and quantified PCV2 DNA in serum, buffy coat, feces, and multiple lymphoid (bronchial, mesenteric, and superficial inguinal lymph nodes; thymus; tonsil; ileal Peyer's patches; and spleen), and non-lymphoid (myocardium; lung; kidney; liver; and gluteal muscle) tissues from naturally infected pigs. Across all tissues and sera of naturally infected pigs, the mean PCV2 concentration was 3.0logs higher in wasting versus non-wasting pigs. PCV2 concentration measured by tissue culture and immunohistochemical staining in homogenized liver samples of experimentally infected gnotobiotic pigs were compared to the concentrations estimated by quantitative PCR. Similar trends were noted with increasing PCV2 concentration detected in subclinically infected to severely PMWS-affected pigs across all assays. Our diagnostic assay was developed with a conserved target sequence, and performed efficiently in quantification of PCV2 in a variety of tissues from naturally and experimentally infected pigs.     

Musculoskeletal injuries in Standardbred racehorses on Prince Edward Island

A study of musculoskeletal injuries in Standardbred racehorses on Prince Edward Island was conducted to determine the incidence and risk factors for injury. Monthly information was collected from 10 trainers and 153 Standardbred horses over a 12-month period. Seventeen new injuries were identified in pacers, representing a horse level incidence risk of 23% and an incidence rate of injury of 2.19 per horse-month at risk. Forty-one percent of the injuries occurred during a race, 53% during training and 6% during jogging. Fourteen horses had experienced a previous injury and 3 of these horses experienced a new injury during the study period. Potential risk factors for injury were the speed at which the horse was trained, previous injury, and the conformational fault of offset knees.     

Microbial communities and their relevance for ecosystem models: Decomposition as a case study

Soil microbial communities are so vastly diverse that complex interactions, which alter ecosystem functions, may occur among microbial species and functional groups. In this review, we explore the empirical evidence for situations when shifts in the community structure of microbes would elicit a change in ecosystem process rates, specifically decomposition, even when microbial biomass remains constant. In particular, we are interested in a subset of these scenarios in which knowledge of microbial community structure would improve model predictions for ecosystem functions. Results from microcosm and field studies indicate that microbial species diversity, functional group diversity, and community composition can all influence ecosystem process rates. The underlying mechanisms that may elicit changes in ecosystem functions from shifts in microbial community structure include evolutionary constraints on microbial trait adaptation, trait correlations, dispersal limitation, and species interactions. The extent of microbial diversity in soils is not known, so it is presently not possible to model all scenarios of microbial community structure shifts. However, by incorporating documented patterns in functional groups that are relevant for a particular ecosystem process and potential relationships between microbial phylogeny and function, the predictive power of process models will be significantly improved. The inclusion of this information into process models is critical for predicting and understanding how ecosystem functions may shift in response to global change.     

Scale dependence in quantification of land-cover and biomass change over Siberian boreal forest landscapes

We investigated the influence of remote sensing spatial resolution on estimates of characteristic land-cover change (LCC) and LCC-related above-ground biomass change (Δbiomass) in three study sites representative of the East Siberian boreal forest. Data included LCC estimated using an existing Landsat-derived land-cover dataset for 1990 and 2000, and above-ground standing biomass stocks simulated by the FAREAST forest succession model and applied on a pixel basis. At the base 60 m resolution, several landscape pattern metrics were derived to describe the characteristic LCC types. LCC data were progressively degraded to 240, 480, and 960 m. LCC proportions and Δbiomass were derived at each of the coarser resolutions and scale dependences of LCC and Δbiomass were analyzed. Compared to the base 60 m resolution, the Logged LCC type was highly scale dependent and was consistently underestimated at coarser resolutions. The Burned type was under- or over-estimated depending strongly on its patch size. Estimated at the base 60 m resolution, modeled biomass increased in two sites (i.e., 3.0 and 6.4 Mg C ha⁻¹ for the Tomsk and Krasnoyarsk sites, respectively) and declined slightly in one site (i.e., −0.5 Mg C ha⁻¹ for the Irkutsk site) between the two dates. At the degraded resolutions, the estimated Δbiomass increased to 3.3 and 7.0 Mg C ha⁻¹ for the Tomsk and Krasnoyarsk sites, while it declined to −0.8 Mg C ha⁻¹ for the Irkutsk site. Results indicate that LCC and Δbiomass values may be progressively amplified in either direction as resolution is degraded, depending on the mean patch size (MPS) of disturbances, and that the error of LCC and Δbiomass estimates also increases at coarser resolutions.     

Carbon dioxide as a potential fumigant for termite control

Formosan subterranean termites, Coptotermes formosanus Shiraki, were exposed to ? 95% or 50% carbon dioxide atmospheres for intervals of 24-120 h at 26(±3)°C. A 24-h exposure to ? 95% carbon dioxide caused significant termite mortality, but 60 h were required for complete mortality. Exposure to 50% carbon dioxide for 60 h resulted in approximately 70% termite mortality, while complete mortality was recorded after 120 h. When termites were sealed in wooden blocks (90 × 90 × 152 mm), 72-96 h exposure to ? 95% carbon dioxide was necessary for complete control. A limited study with Cryptotermes brevis (Walker) suggested that this drywood termite is also susceptible to carbon dioxide fumigation, although slightly longer exposures may be required than with C. formosanus. Carbon dioxide-modified atmospheres are a viable alternative to conventional fumigants for vault fumigation of termite-infested materials, and may also be applicable to larger-scale fumigations to control structural pests.