加强家禽疫情监测保障产业健康发展

家禽产业的发展离不开生物安全措施的保障,其中对疫情的监测十分重要,不仅影响家禽的生产性能,而且关系到产业的健康发展。作者以禽流感的监测为例,为家禽疫病的监测提供了良好的指导和借鉴。     

上海轨道公安警犬国庆执勤记

2007年9月30日至10月3日,笔者随警作战,记录下上海市轨道公安分局警犬大队29名民警参加国庆安保工作的过程。整理这段笔记时,阵阵发自内心深处的震撼在灵魂深处激荡。这是一次在挑战面前的完美"亮剑",在关键时刻的刚毅"突击"。     

温室授粉蜂群蜂子比值与工蜂出勤率相关性的研究

蜜蜂是设施农业的重要授粉昆虫,授粉蜂群的状况可能影响授粉效率的高低。通过意大利蜂(Apismellifera)对温室草莓、油桃的6次授粉重复试验,采集授粉蜂群的蜂子比值与出勤率的数据,分析蜂子比值与出勤率的相关性。结果表明,蜂子比值与工蜂出勤率之间呈显著的负相关(P<0.05),其相关系数分别为-0.8470、-0.6543、-0.7616、-0.6969、-0.6740和-0.8477,说明在正常范围内,温室授粉蜂群的出勤率随蜂子比值的减小而提高,也就是说,授粉蜂群内的子脾越好,蜜蜂出勤采集的积极性越高。     

扎龙自然保护区鸟类观赏性分析与观鸟管理对策

观鸟是一项陶冶身心、消除疲劳、接近大自然、科学性很强的活动。扎龙国家级自然保护区内，鸟类资源丰富，是进行观鸟旅游和科普、科研的重要基地，本文主要构建了鸟类观赏性评估体系，并对保护区的鸟类进行了观赏性评估；通过问卷调查分析了观鸟者对自然保护区和鸟类的认知度、喜爱度和观鸟的期望等等；并为保护区更好地开展观鸟旅游和科研、科普活动提出管理建议。     

Interspecific relationships of Lycoris (Amaryllidaceae) inferred from inter-simple sequence repeat data

Inter-simple sequence repeat (ISSR) markers were used to evaluate interspecific relationships of Lycoris. Twenty-four samples were included in the study representing a total of 20 among species and varieties. Thirteen primers produced 228 discernible DNA fragments, 205, or 89.91%, of which were polymorphic, indicating a high level of interspecific genetic variation in Lycoris. Our UPGMA cluster analysis recognized four major groups of species, which were consistent with morphological and karyotype observations. The first group included species with telocentric and metacentric chromosomes, while the second consisted of species with a haploid genome of 11 subtelocentric chromosomes. The third group contained species with a mixture of subtelocentric, telocentric and metacentric chromosomes. The last group included the Japanese and Korean species. Lycoris anhuiensis was clustered within accessions of Lycoris longituba, suggesting that it could be recognized as a variety of L. longituba. Our ISSR data also suggested that L. straminea may be a hybrid between Lycoris chinensis and Lycoris radiata var. pumila, and that L. caldwellii, an allotriploid, may be of a hybrid origin of L. chinensis and L. sprengeri.     

顶尖防除春小麦田野燕麦的效果

用15%顶尖可湿性粉剂200~300 g/hm2,对水300 kg/hm2,于小麦苗期茎叶喷雾,对春小麦田野燕麦防除效果达95%以上,增产20%以上,与对照药剂6.9%骠马EW 750 m l/hm2除草效果相当。     

添加有机硅表面活性剂对低容量喷雾防治小麦蚜虫的影响

室内试验表明,有机硅表面活性剂Silwet408、非离子表面活性剂OP-10和JFC可显著降低药液的表面张力,其中Silwet408在质量分数为0.05%时可使水的表面张力降到22.05 mN/m,表面活性最强。当药液中含有0.001%的有机硅表面活性剂Silwet408时,其在小麦叶片上的接触角即开始显著减小,且在小麦背面的接触角小于在正面的接触角。在10%吡虫啉可湿性粉剂稀释2500倍药液中添加Silwet408,药液的表面张力和其在小麦叶片上的接触角明显降低,雾滴在小麦叶片上扩展面积明显增大。进一步田间试验表明,使用手持离心式低容量喷雾机喷雾,在10%吡虫啉可湿性粉剂药液中添加质量分数为0.05%和0.1%的Silwet408,可显著增加对小麦蚜虫的防治效果。     

油菜籽皮发酵饲料添加剂——纤维素酶

本研究对绿色木霉116的发酵条件和无机盐配方进行了正交试验优选。试验结果表明产纤维素酶的最佳条件是:接种量为2%,油菜籽皮:稻草:麸皮=3:2:5;水分:干料=1:1;装量为5%;无机盐固态发酵的最佳配方是:(NH4)2SO40.5%,KH2PO40.4%,MgSO40.04%,CaCl2·2H2O0.05%。产酶酶活可达242U/g鲜糟。     

Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD

Improved protocol for Agrobacterium mediated transformation of tomato (Lycopersicon esculentum) Micro-Tom was developed to use in corporation of the carotenoid biosynthetic genes CsZCD (Crocus zeaxanthin 7,8-cleavage dioxygenase). From these experiment, a transformation methodology using explants from cotyledons cultured for 1 day on the medium with zeatin 2 mg/L, IAA 0.1 mg/L, carefully submerged in the Agrobacterium inoculum for 20 min, then concultured with the agrobacterium for 3 days on the same medium, followed by a transfer to the same medium with 500 mg/L cefotaxin for 3 days and then by a transfer to the same medium with 100 mg/L kanamycin and 500 mg/L carabenillin for 6–8 weeks and resulted in a greater than 20% transformation efficiency in the concentration of Agrobacterium OD600 = 0.2 tested. In this transformation method, no feeder layers were used and the subculture media was minimal. Among the Agrobacterium concentrations of OD600 = 0.2, 0.5 and 1.0, the best transformation efficiency, 20.87%, was obtained by using OD600 = 0.2, which was significantly higher than that of OD600 = 1.0. The presence of the inserted target genes was checked using a rapid and efficient PCR test. The protocol was successfully employed in the production of transgenic Micro-Tom tomato containing the carotenoid biosynthesis CsZCD under constructive promoter. This procedure represents a simple, efficient and general means of transforming tomato.     

辽西地区相对资源承载力与可持续发展研究

本文讨论并改进了相对资源承载力的概念、研究思路和计算方法,选择具有代表性的辽西地区,实证分析1988—2004年辽西地区相对土地资源承载力、相对经济资源承载力、相对生活水平承载力和综合承载力的演变和空间差异,预测2010年和2015年辽西地区的人口超载规模,分析辽西地区相对资源承载力存在问题的原因,提出辽西地区实现可持续发展的对策。