Low-temperature synthesis of zintl compounds with a single-source molecular precursor

Thermolysis of the heterobimetallic phosphinidene complex [Sb(PCy)3]2- Li6.6HNMe2 (Cy = C6H11) at 303 to 313 kelvin gives Zintl compounds containing (Sb7)3- anions. The complex thus constitutes a stable molecular single-source precursor to Zintl compounds and provides a potential low-temperature route to photoactive alkali metal antimonates. The new chemical reaction involved, which is driven thermodynamically by the formation of P-P bonds, has implications in the low-temperature synthesis of other technologically important materials (such as gallium arsenide).     

Homogeneous Linewidths in the Optical Spectrum of a Single Gallium Arsenide Quantum Dot

The homogeneous linewidths in the photoluminescence excitation spectrum of a single, naturally formed gallium arsenide (GaAs) quantum dot have been measured with high spatial and spectral resolution. The energies and linewidths of the homogeneous spectrum provide a new perspective on the dephasing dynamics of the exciton in a quantum-confined, solid-state system. The origins of the linewidths are discussed in terms of the dynamics of the exciton in zero dimensions, in particular, in terms of lifetime broadening through the emission or absorption of phonons and photons.     

Early Development and Putative Primordial Germ Cells Characterization in Dogs

Previously, three distinct populations of putative primordial germ cells (PGCs), namely gonocytes, intermediate cells and pre‐spermatogonia, have been described in the human foetal testis. According to our knowledge, these PGCs have not been studied in any other species. The aim of our study was to identify similar PGC populations in canine embryos. First, we develop a protocol for canine embryo isolation. Following our protocol, 15 canine embryos at 21–25 days of pregnancy were isolated by ovaryhysterectomy surgery. Our data indicate that dramatic changes occur in canine embryo development and PGCs specification between 21 to 25 days of gestation. At that moment, only two PGC populations with distinct morphology can be identified by histological analyses. Cell population 1 presented round nuclei with prominent nucleolus and a high nuclear to cytoplasm ratio, showing gonocyte morphology. Cell population 2 was often localized at the periphery of the testicular cords and presented typical features of PGC. Both germ cell populations were positively immunostained with anti‐human OCT‐4 antibody. However, at day 25, all cells of population 1 reacted positively with OCT‐4, whereas in population 2, fewer cells were positive for this marker. These two PGCs populations present morphological features similar to gonocytes and intermediate cells from human foetal testis. It is expected that a population of pre‐spermatogonia would be observed at later stages of canine foetus development. We also showed that anti‐human OCT‐4 antibody can be useful to identify canine PGC in vivo.     

Validation of a methodology for grouping intakes of pressurized irrigation networks into sectors to minimize energy consumption

A methodology to optimise the amount of energy consumed in pressurized irrigation systems was presented by Jimenez-Bello et al. (2010a). These authors proposed grouping pressurized irrigation network intakes, each of the water turnouts resulting from a shared hydrant, into sectors via a genetic algorithm. In the present research, the methodology was applied and validated in a water users association. Several energy efficiency indicators were calculated and compared during five consecutive seasons (2006–2010). The first two seasons, when the methodology was not employed, were used as reference for the results obtained from 2008 onwards, when the methodology was applied to the management of irrigation network. Results obtained in seasons 2008–2010 showed that the average energy savings were 16% in comparisons to the 2006 season. However, it should be noted that the potential, theoretical savings, could have been as high as 22.3% if the modelled grouping networks would have been accurately followed. There was in fact some discrepancy between the theoretical model outputs and the final groupings due to some intake restrictions. In addition, during the irrigation campaigns, the number of irrigation intakes that operated within each sector was not always equal to the modelled sectoring, a fact that reduced the overall water users association energy efficiency. This occurred particularly during rainy periods, when some users deliberately decided to close their manual irrigation intakes valves. Overall, results showed the potential of the validated methodology for optimising energy use. However, the final overall system efficiency might depend on specific constraints that need to be taken into account when attempting to use model output predictions.     

Development and validation of an automatic thermal imaging process for assessing plant water status

Leaf temperature is a physiological trait that can be used for monitoring plant water status. Nowadays, by means of thermography, canopy temperature can be remotely determined. In this sense, it is crucial to automatically process the images. In the present work, a methodology for the automatic analysis of frontal images taken on individual trees was developed. The procedure can be used when cameras take at the same time thermal and visible scenes, so it is not necessary to reference the images. In this way, during the processing in batch, no operator participated. The procedure was developed by means of a non supervised classification of the visible image from which the presence of sky and soil could be detected. In case of existence, a mask was performed for the extraction of intermediate pixels to calculate canopy temperature by means of the thermal image. At the same time, sunlit and shady leaves could be detected and isolated. Thus, the procedure allowed to separately determine canopy temperature either of the more exposed part of the canopy or of the shaded portion. The methodology developed was validated using images taken in several regulated deficit irrigation trials in Persimmon and two citrus cultivars (Clementina de Nules and Navel Lane-Late). Overall, results indicated that similar canopy temperatures were calculated either by means of the automatic process or the manual procedure. The procedure developed allows to drastically reduce the time needed for image analysis also considering that no operator participation was required. This tool will facilitate further investigations in course for assessing the feasibility of thermography for detecting plant water status in woody perennial crops with discontinuous canopies. Preliminary results reported indicate that the type of crop evaluated has an important influence in the results obtained from thermographic imagery. Thus, in Persimmon trees there were good correlations between canopy temperature and plant water status while, in Clementina de Nules and Navel Lane-Late citrus cultivars canopy temperature differences among trees could not be related with tree-to-tree variations in plant water status.     

Water relations of field grown Pomegranate trees (Punica granatum) under different drip irrigation regimes

Pomegranate (Punica granatum L.) is a deciduous fruit tree native of central Asia included in the so-called group of minor fruit tree species, not widely grown but of some importance in the south east of Spain. Fruit consumption interest is due to the organoleptic characteristics and to the beneficial effects on health. Pomegranate tree are considered as a culture tolerant to soil water deficit. However, very little is known about pomegranate orchard water management. The objective of this work was to characterize, for the first time in P. granatum, water relations aspects of applied significance for irrigation scheduling. Trees under different irrigation regimes were used and midday stem water potential (Ψ_stem) and midday leaf gas exchange were periodically measured over the course of an entire season. During spring and autumn, Ψ_stem did not show significant differences between irrigation treatments while there were considerable differences in leaf photosynthesis and stomatal conductance, suggesting a near-isohydric behaviour of pomegranate trees. This might explain why the signal intensity of Ψ_stem was lower than those of gas exchange indicators during the experimental period. Thus, leaf photosynthesis rates and stomatal conductance might have a greater potential for irrigation scheduling of pomegranate trees than Ψ_stem measured at solar noon.     

A cross-sectional pilot study to estimate the prevalence of and risk factors for leptospirosis in South-Western Victorian dairy herds, 2017

Leptospirosis is a zoonosis, found worldwide, affecting many species of animals. We conducted a cross-sectional study to estimate the prevalence of Leptospira borgpetersenii sv Hardjo and Leptospira interrogans sv Pomona in cattle in dairy herds in South-Western Victoria, Australia. Fifty-three herds were enrolled in the study. Urine samples were collected from 15 late-lactation cows in each herd. A questionnaire was provided to herd managers at the time of each herd visit, asking them to describe the methods they used for controlling leptospirosis, including vaccination. Urine samples were pooled at the herd level and tested for leptospira spp. using real time PCR. Urine samples from individual cows within the positive pooled samples were then tested for Leptospira Hardjo and Leptospira Pomona using qPCR. Four of the 53 herds showed positive leptospirosis results giving an apparent prevalence of 8 (95% CI 2–18) leptospira-positive herds per 100 herds at risk. Based on the 53 completed questionnaires, leptospirosis vaccination programs were not compliant with label directions in 36 of the 52 vaccinated herds: 69 (95% CI 55–81) of 100 herd managers that routinely vaccinated for leptospirosis did not comply with label directions. One herd was completely unvaccinated. Based on our findings, we estimate that approximately 10% of dairy farms in South-Western Victoria are likely to be infected with leptospirosis. While most herds are vaccinating for leptospirosis, most are not doing so according to label directions. We conclude that herd managers need to be better educated regarding leptospirosis vaccination programs.